Results 111 to 120 of about 4,284,812 (296)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

The use of blockchain technology for preserving cultural heritage and its impact on political processes

open access: yesRUDN Journal of Public Administration
One of the pressing issues in the modern world is the problem of trust in authority. Cultural heritage and the challenges associated with its acknowledgment and preservation often directly influence political processes both within countries and in ...
Denis A. Gromyko
doaj   +1 more source

GENERAL ORIENTATIONS OF THE MANAGEMENT OF PUBLIC INSTITUTIONS IN THE CONTEXT OF THE KNOWLEDGE SOCIETY [PDF]

open access: yes
Romanian public administration and new public management are in need of a series of modernization actions resulted from theoretical progresses on European level whose application proved to have positive effects.
Adelina DUMITRESCU, Mihail DUMITRESCU
core  

Anticancer sensitivities and biological characteristics of HCT116 cells resistant to the selective poly(ADP‐ribose) glycohydrolase inhibitor

open access: yesFEBS Open Bio, EarlyView.
We analyzed alterations of PAR metabolism‐related proteins in PARG inhibitor‐resistant HCT116RPDD cells. Although PARG levels remained unchanged, HCT116RPDD cells exhibited reduced PARP1 and ARH3 expression and elevated PAR levels. Interestingly, HCT116RPDD cells exhibited slightly elevated intracellular NAD+/NADH and ATP levels. Our findings suggest a
Kaede Tsuda, Yoko Ogino, Akira Sato
wiley   +1 more source

A model for prevocational training of specialists: managing the “Media class in Moscow schools” project as an example of implementing state and municipal education policy

open access: yesRUDN Journal of Public Administration
The study presents an analysis of the development of pre-professional education in Moscow with an emphasis on the implementation of the Media Class in a Moscow School project, which has become an example of successful integration of media technologies ...
Andrey V. Sazonov   +2 more
doaj   +1 more source

Institutional governance barriers for the development and implementation of climate adaptation strategies [PDF]

open access: yes, 2009
In this paper we summarise the main social barriers to adaptation presented in climate literature – the inherent uncertainty of climate change, fragmentation, institutional voids, short term horizon of politicians and policies, and the motives and ...
Biesbroek, G.R.   +3 more
core   +1 more source

Differential regulation of ZFAS1 splice variants by endoplasmic reticulum stress in hepatocyte cell lines

open access: yesFEBS Open Bio, EarlyView.
ZFAS1 is a lncRNA promoting cell proliferation and migration, exhibiting high expression in various cancers. It is conserved, widely expressed, and produces multiple splice variants with unclear roles. We identified several splice variants in hepatocyte models, and found that inhibiting or suppressing regulators of the unfolded protein response (PERK ...
Sébastien Soubeyrand   +2 more
wiley   +1 more source

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