Results 121 to 130 of about 1,670,878 (298)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Analyzing Public Transport Wait Times and Identifying the Most Affected Users in the Metropolitan Area of Valparaíso, Chile

Applied Sciences
Public transportation wait times are a crucial factor influencing users’ perception of the system’s efficiency, their satisfaction, and their willingness to continue using these services.
Felipe González, Vicente Aprigliano, Sebastian Seriani, Alvaro Peña   +3 more
doaj   +1 more source

Tidewater metropolitan area transportation survey study [PDF]

Results of a survey conducted in southeastern Virginia to identify public opinion regarding requirements for future public transportation are analyzed. The effects of age and income level on attitudes concerning city living and no importance of various ...
Ficht, J. C.
core   +1 more source

Tumor‐stromal crosstalk and macrophage enrichment are associated with chemotherapy response in bladder cancer

FEBS Open Bio, EarlyView.
Chemoresistance in bladder cancer: Macrophage recruitment associated with CXCL1, CXCL5 and CXCL8 expression is characteristic of Gemcitabine/Cisplatin (Gem/Cis) Non‐Responder tumors (right side) while Responder tumors did not show substantial tumor‐stromal crosstalk (left side). All biological icons are attributed to Bioicons: carcinoma, cancerous‐cell‐
Sophie Leypold, Janik Riese, Lancelot Seillier, Mark Kühnel, Julia Pannhausen, Charlotte O. J. Fröhlich, Christian Martin, Peter Boor, Matthias Saar, Danny D. Jonigk, Nadine T. Gaisa, Michael Rose   +11 more
wiley   +1 more source

Differential regulation of ZFAS1 splice variants by endoplasmic reticulum stress in hepatocyte cell lines

FEBS Open Bio, EarlyView.
ZFAS1 is a lncRNA promoting cell proliferation and migration, exhibiting high expression in various cancers. It is conserved, widely expressed, and produces multiple splice variants with unclear roles. We identified several splice variants in hepatocyte models, and found that inhibiting or suppressing regulators of the unfolded protein response (PERK ...
Sébastien Soubeyrand, Paulina Lau, Ruth McPherson   +2 more
wiley   +1 more source

Unveiling transportation disparities: investigating accessibility gaps in metropolitan cities using GIS—a case study of Alexandria, Egypt

Frontiers in Sustainable Cities
IntroductionPublic transportation is essential to reduce pollution and traffic congestion in urban areas. However, in densely populated regions like Alexandria, characterized by high population density and limited road infrastructure, accessibility to ...
Sally ElDeeb, Rowan Adel Abd EL-Baky, Houshmand Masoumi, Houshmand Masoumi   +3 more
doaj   +1 more source

Anchorage‐independent and faster growth in clonal population from UV‐irradiated NER‐deficient cells

FEBS Open Bio, EarlyView.
UV‐irradiated cells expressing a DDB2 mutant protein unable to interact with PCNA (DDB2PCNA‐) form clones able to grow without anchorage. Different experimental approaches reveal heterogeneity in cell cycle regulation and drug response within these clones, emphasizing the crucial role of the DDB2‐PCNA interaction in preventing cellular transformation ...
Paola Perucca, Anna Tricarico, Martina Furfaro, Priyam Ghosh, Ennio Prosperi, Lucia Anna Stivala, Ornella Cazzalini   +6 more
wiley   +1 more source

Metro Train Stopping Scheme Decision Based on Multisource Data in Express-Local Train Mode

Journal of Advanced Transportation
The urban rail transit network has gradually realized grid operation with the increase in the coverage rate. Therefore, the stopping schemes in accordance with the trend of the passenger flow are more conducive to improving the attractiveness of the rail
Jin Li, Yaqiu Wang, Shiyin Zhang, Huasheng Liu   +3 more
doaj   +1 more source

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

Midwest Transportation Consortium Annual Program Report, October 2004 [PDF]

, 2004
The MTC’s main focus is on education and human capital. This focus is in recognition of the fact that the transportation industry, both public and private, in the region served by the MTC faces a serious shortage of well-trained human capital.

core