Results 21 to 30 of about 72,875 (296)

Genetic similarity of Burkholderia cenocepacia from cystic fibrosis patients

Brazilian Journal of Infectious Diseases, 2013
Burkholderia cenocepacia may cause serious infections in patients with cystic fibrosis, and this microorganism can be highly transmissible. Pulsed-field gel electrophoresis is widely used to study the dynamics of strain spread in cystic fibrosis patients.
Luana Pretto, Fernanda de-Paris, Alice Beatriz Mombach Pinheiro Machado, Andreza Francisco Martins, Afonso Luís Barth   +4 more
doaj   +3 more sources

Agarose Gel Electrophoresis-Based RAPD-PCR—An Optimization of the Conditions to Rapidly Detect Similarity of the Alert Pathogens for the Purpose of Epidemiological Studies

Gels, 2022
Agarose gel electrophoresis is a well-known tool to detect DNA fragments amplified in polymerase chain reaction (PCR). Its usefulness has also been confirmed for epidemiological studies based on restriction fragments length polymorphism (RFLP), usually ...
Tomasz Bogiel, Agnieszka Mikucka, Piotr Kanarek   +2 more
doaj   +1 more source

Pulse Field Gel Electrophoresis [PDF]

, 2014
Pulse Field Gel Electrophoresis (PFGE) is a powerful genotyping technique used for the separation of large DNA molecules (entire genomic DNA) after digesting it with unique restriction enzymes and applying to a gel matrix under the electric field that periodically changes direction.
Batu K, Sharma-Kuinkel, Thomas H, Rude, Vance G, Fowler   +2 more
openaire   +2 more sources

Application of Pulsed Field Gel Electrophoresis to Determine γ-ray-induced Double-strand Breaks in Yeast Chromosomal Molecules [PDF]

, 1993
The frequency of DNA double-strand breaks (dsb) was determined in yeast cells exposed to γ-rays under anoxic conditions. Genomic DNA of treated cells was separated by pulsed field gel electrophoresis, and two different approaches for the evaluation of ...
A.A. Friedl, A.M. Kellerer, Ager D.D., Ager D.D., Ahn S.Y., Andrews J., Blazek E.R., Blöcher D, Blöcher D, Blöcher D., Blöcher D., Blöcher D., Bronstein I., Contopoulou C.R., F. Eckardt-Schupp, Frankenberg D., Frankenberg-Schwager M., Geigl E.-M., Geigl E.-M., Geigl E.-M., Geigl E.-M., Hanawalt P.C, Ho K.S.Y, Iliakis G.E., Iliakis G.E., Jacobs A., K. Hahn, Kessler B., Kunz B.A., Lennartz M., Okayasu R.B., Oleinick N.L., Radford I.R., Resnick M.A., Schulz G.V, Smith L.C., Stamato T.D., Terleth C., W. Beisker, Ward J.F   +39 more
core   +1 more source

Molecular characterization of Salmonella Enteritidis : comparison of an optimized multi-locus variable-number of tandem repeat analysis (MLVA) and pulsed-field gel electrophoresis [PDF]

, 2012
Salmonella Enteritidis (SE) is a genetically homogenous serovar, which makes optimal subtype discrimination crucial for epidemiological research. This study describes the development and evaluation of an optimized multiple-locus variable number tandem ...
Bertrand, Sophie, De Reu, Koen, Dewaele, Isabelle, Ducatelle, Richard, Herman, Lieve, Heyndrickx, Marc, Imberechts, Hein, Rasschaert, Geertrui, Wattiau, Pierre, Wildemauwe, Christa   +9 more
core   +2 more sources

Agarose Gel Analysis of 15–40-kb PCR Amplimers

BioTechniques, 1996
Accurate resolution of PCR products in the range of 15–40 kb may be obtained in agarose gels without pulsed-field electrophoresis. A gel of 0.3% SeaKem® Gold agarose cast on GelBond® support film provides good resolution and sufficient gel strength to ...
Valerie Burland, Foner P. Curtis, Noriko Kusukawa   +2 more
doaj   +1 more source

Epidemiological analysis of methicillin-resistant Staphylococcus aureus isolates from adult patients with cystic fibrosis [PDF]

, 2006
Using pulsed-field gel electrophoresis, we genotyped 21 methicillin-resistant Staphylococcus aureus isolates from patients attending an adult cystic fibrosis unit. Eleven patients exhibited pulsotypes related to 2 locally endemic strains.
C. Coulter, French, Govan, S. C. Bell, T. J. Kidd, Tenover, Wood   +6 more
core   +1 more source

Development and evaluation of a novel, semiautomated Clostridium difficile typing platform [PDF]

, 2013
We describe a novel, semiautomated Clostridium difficile typing platform that is based on PCR-ribotyping in conjunction with a semiautomated molecular typing system. The platform is reproducible with minimal intra- or interassay variability.
Burnham, Carey-Ann D, Chamberland, Robin R, Collins, Rachel, Dubberke, Erik R, Dunne, W. Michael, Jr., MacCannell, Duncan, Westblade, Lars F   +6 more
core   +2 more sources

Nosocomial Transmission of Group B Streptococci Proven by Positive Environmental Culture

Oman Medical Journal, 2014
Objective: Neonates usually acquire Group B streptococcal infection vertically from the maternal birth canal during delivery. In January 2010, a Group B streptococcal outbreak investigation was conducted in response to an increased number of clinical ...
Amal Al-Maani, Laurie Streitenberger, Megan Clarke, Yvonne C. W. Yau, Danuta Kovach, Rick Wray, Anne Matlow   +6 more
doaj   +1 more source

Streptococcus pneumoniae serotype 3 genotypes in invasive isolates from Colombia

Biomédica: revista del Instituto Nacional de Salud, 2021
Introduction: Streptococcus pneumoniae serotype 3 is an important cause of pneumonia, bacteremia, and meningitis. Objective: To establish the circulating genotypes of S.
Zonia Katerin Alarcón, Carolina Duarte, Olga Sanabria, Jaime Moreno   +3 more
doaj   +1 more source