Application of Pulsed Field Gel Electrophoresis to Determine γ-ray-induced Double-strand Breaks in Yeast Chromosomal Molecules [PDF]
, 1993 The frequency of DNA double-strand breaks (dsb) was determined in yeast cells exposed to γ-rays under anoxic conditions. Genomic DNA of treated cells was separated by pulsed field gel electrophoresis, and two different approaches for the evaluation of ...
Beisker, W. +8 more
core +1 more source
Localization of the genes for tumor necrosis factor and lymphotoxin between the HLA classI and III regions by field inversion gel electrophoresis [PDF]
, 1988 To clarify the position of the TNFA and TNFB genes on the HLA map, we have assigned TNFA to large DNA restriction fragments separated by field inversion gel electrophoresis, which hybridize with either class III- or class I-specific probes as well. These
Bloemer, K +7 more
core +1 more source
A Physical Map Including a New Class I Gene (cda12) of the Human Major Histocompatibility Complex (A2/313 Haplotype) Derived from a Monosomy 6 Mutant Cell Line [PDF]
, 1989 To avoid interpretative problems due to restriction fragment length polymorphisms, the monosomy 6 mutant cell line BM19.7 was employed to establish a molecular map of the human major histocompatibility (HLA) complex in the A2,B13,Bw4,DRw6,DRw52,DQw1,DPw2
Pohla, Heike +11 more
core +1 more source
Pulsed field gel electrophoresis protocol for typing of uropathogenic Escherichia coli [PDF]
فصلنامه دانشگاه علوم پزشکی جهرم, 2011 Introduction: Pulsed Field Gel Electrophoresis (PFGE) is one of the best typing methods which is highly effective in molecular epidemiological studies in different areas. All bacterial isolates are typeable by PFGE and the results are highly reproducible.
Mojtaba Anvarinejad +4 more
doaj +1 more source
Computer simulation of pulsed field gel runs allows the quantitation of radiation-induced double-strand breaks in yeast [PDF]
, 1994 A procedure for the quantification of double-strand breaks in yeast is presented that utilizes pulsed field gel electrophoresis (PFGE) and a comparison of the observed DNA mass distribution in the gel lanes with calculated distributions.
Kraxenberger, Alfred +5 more
core +1 more source
Epidemiological Typing of Moraxella Catarrhalis by Pulsed Field Gel Electrophoresis
Canadian Journal of Infectious Diseases, 1995 Pulsed field gel electrophoresis (pfge) was used to compare 59 strains of Moraxella catarrhalis to evaluate pfge for the epidemiological typing of this organism.
Susan M Davison +4 more
doaj +1 more source
Biomédica: revista del Instituto Nacional de Salud, 2013 Introducción. La leptospirosis es una infección bacteriana transmitida directa o indirectamente de animales a humanos, la cual puede resultar en una enfermedad hemorrágica grave, hepática o renal y pulmonar.
Claudia M. Romero-Vivas +8 more
doaj +1 more source
Pulsed Field Gel Electrophoresis of Group A Streptococci [PDF]
, 2015 Here we describe the protocols to perform PFGE analysis of chromosomal DNA from the bacterial species Streptococcus pyogenes (group A streptococcus, GAS) after digestion with the restriction enzyme SmaI. Large parts of the procedures are suitable for application to DNA digested with other restriction enzymes as well.
VITALI, Luca Agostino +2 more
openaire +3 more sources
Van Tıp Dergisi, 2022 INTRODUCTION: It was aimed to determine the presence of metallo-beta-lactamases (MBL) and oxacillinases (OXA) enzymes in Acinetobacter baumannii complex (ABC) isolates, which show decreased sensitivity to carbapenems isolated from blood cultures and to ...
Gülşen Uluçam Atay +5 more
doaj +1 more source
Lactococcus lactis subsp. lactis Infection in Waterfowl: First Confirmation in Animals
Emerging Infectious Diseases, 2001 We report the first description, confirmed by bacteriologic and molecular (polymerase chain reaction and pulsed-field gel electrophoresis) analysis, of an infection in animals caused by Lactococcus lactis subsp. lactis, affecting waterfowl.
Joaquín Goyache +9 more
doaj +1 more source