Results 181 to 190 of about 30,835 (252)

Transient Transfection of Rolling‐Circle Amplified DNA in Biomanufacturing‐Relevant Mammalian Cell Lines: A Comparison of Transfection Conditions for Optimal Protein Expression

Biotechnology and Applied Biochemistry, Volume 73, Issue 3, Page 1358-1367, June 2026.
ABSTRACT Rolling‐circle amplification (RCA) is a completely synthetic and rapid mechanism for scaling‐up bulk DNA that eliminates requirements for large‐scale bacterial fermentation. Several reports have described the surprising ability of large unprocessed (hyperbranched) rolling circle‐amplified DNA (RCA DNA) to effectively transfect cultured cells ...
Evelina Loghin, Erik Kvam, Lisa Lowery, Nathan Hudson, Jenny Keen, Brian Davis, Roger B. Dodd, Christine Surrette, John Nelson   +8 more
wiley   +1 more source

Molecular Epidemiology of Mycobacterium tuberculosis Isolates in 100 Patients With Tuberculosis Using Pulsed Field Gel Electrophoresis. [PDF]

Jundishapur J Microbiol, 2015
Pooideh M, Jabbarzadeh I, Ranjbar R, Saifi M.   +3 more
europepmc   +1 more source

Hydrogen Sulfide Rescues Microglia From HIV Tat‐Driven Ferroptosis: Implications for HIV‐Associated Neuroinflammation

CNS Neuroscience &Therapeutics, Volume 32, Issue 6, June 2026.
HIV Tat induces ferroptosis and proinflammatory activation in microglia through iron accumulation and oxidative stress. Hydrogen sulfide donor NaHS suppresses HIV Tat‐induced ferroptosis by restoring redox balance, reinforcing the SLC7A11‐GPX4 axis, and attenuating microglial inflammation, highlighting hydrogen sulfide as a potential therapeutic ...
Aitizaz Ul Ahsan, Frida L. Martínez‐Cuevas, Elias Horanieh, Seema Singh, Uma Maheswari Deshetty, Palsamy Periyasamy, Shilpa Buch   +6 more
wiley   +1 more source

Trapping and breaking of in vivo nicked DNA during pulsed field gel electrophoresis. [PDF]

Anal Biochem, 2013
Khan SR, Kuzminov A.
europepmc   +1 more source

Mitochondrial Genome of the Indo‐Pacific Mesophotic Coral Leptoseris columna (Scleractinia: Agariciidae) Assembled Using PacBio Long‐Read Sequencing

Ecology and Evolution, Volume 16, Issue 6, June 2026.
The complete mitochondrial genome of Leptoseris columna was assembled using PacBio long read sequencing. ABSTRACT Leptoseris columna, a mesophotic coral species belonging to the family Agariciidae, is distributed throughout the Indo‐Pacific region.
Nomita Rani Adhikary, Daniel J. Barshis, J. Antonio Baeza   +2 more
wiley   +1 more source

Comparison of restriction enzymes for pulsed-field gel electrophoresis typing of Moraxella catarrhalis. [PDF]

J Clin Microbiol, 2013
Marti S, Puig C, Domenech A, Liñares J, Ardanuy C.   +4 more
europepmc   +1 more source

Advances in High‐Throughput Sequencing: Antimicrobial Resistance and Traceability of Foodborne Pathogens

eFood, Volume 7, Issue 3, June 2026.
In this graphical abstract, the transfer experiment of antimicrobial resistance genes and metagenomic analysis are presented, and they are the core components of relevant microbial research. ABSTRACT High‐throughput sequencing technology (HTST) can effectively analyze the sequence information in the DNA of samples and provide a comprehensive ...
Kexuan Wu, Jie Zhao, Ningxin Huang, Zhengxiao Wang, Yiping Xu, Shaoning Liu, Chuanlin Bi, Juan Tang, Wei Wu   +8 more
wiley   +1 more source

Capillary Electrophoresis With Amperometric Detection for Neurotransmitter Analysis: Principles, Electrode Materials, Methodologies, and Applications

ELECTROPHORESIS, Volume 47, Issue 6, Page 450-478, June 2026.
This review provides a comprehensive summary of monoamine neurotransmitter analysis by capillary electrophoresis with amperometric detection. Fundamental principles of amperometric detection, electrode positioning strategies, and approaches to high‐voltage decoupling are described, followed by a critical comparison of electrode materials, selection of ...
Petr Kubáň, Jiří Volánek, Nguyen Thi Thu Trang, Tran Dai Lam   +3 more
wiley   +1 more source

Functional and physicochemical properties of proteins isolated from four different pulses

Food Biomacromolecules, Volume 3, Issue 2, Page 281-292, June 2026.
Proteins from four pulses viz. pinto bean, cow pea, pigeon pea, and navy bean were isolated and characterized. Pinto bean protein showed the highest oil absorption capacity and solubility. Pinto bean protein exhibited the highest emulsion capacity and foaming capacity. Cow pea protein showed the highest in vitro protein digestibility.
Bharati Kumari, Nandan Sit
wiley   +1 more source