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Pulsed-field gel electrophoresis
1992In view of the great impact of restriction fragment length polymorphism (RFLP) markers on both basic and applied plant research, considerable effort is currently being spent in constructing complete genetic linkage maps of the genomes of a wide variety of plant species.
Raymond Van Daelen, Pim Zabel
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Pulsed-field gel electrophoresis.
Methods in enzymology, 1996Campylobacter is a common foodborne pathogen. In most outbreaks, the source of infection is not found, due to the time passing from infection to diagnosis. In processed foods, Campylobacter may also become stressed and difficult to culture. In milkborne outbreaks, the contaminated milk is usually not kept long enough, and the bulk milk is not ...
J C, Wrestler +3 more
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Theories of Pulsed-Field Gel Electrophoresis
2003Pulsed-field gel electrophoresis (PFGE) is one of the key technological advances of the past ten years that has made the mapping of genomes of whole organisms possible. In conventional electrophoreis, the mobility of DNA at almost any practical value of the field strength is essentially independent of mol wt above approx 30 kbp.
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Pulsed-field gel electrophoresis
Genome, 1989Pulsed-field gel electrophoresis is a technique making its first impact at the International Congress of Genetics meetings. Much progress has been made since the first demonstrations of this revolutionary technique separating intact yeast chromosomes (Schwartz et al. 1983).
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Pulse Field Gel Electrophoresis
2015International ...
Jordan, Kieran, Dalmasso, Marion
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Pulsed Field Gel Electrophoresis
1995Abstract Pulsed field gel electrophoresis (PFGE) is the most reliable method for separating large fragments of DNA and has had a significant impact on the analysis of both prokaryotic and eukaryotic genomes. This book presents detailed chapters on both the theory behind PFGE and protocols for most of its major research and diagnostic ...
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Pulsed-Field Gel Electrophoresis of Staphylococcus aureus
2015For many bacterial pathogens, including Staphylococcus aureus, pulsed-field gel electrophoresis (PFGE) is a molecular typing method widely used in surveillance and epidemiological investigations. The general principle of PFGE involves creating large DNA fragments from intact bacterial chromosomes using rare cutting restriction endonucleases.
George R, Golding +3 more
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Pulsed-Field Gel Electrophoresis of Yersinia pestis
2015Yersinia pestis is a human pathogen and can cause serious disease. Biosafety level 3 (BSL3) is required when handling this microorganism and all work requires a biological safety cabinet. For pulsed-field gel electrophoresis (PFGE), dedicated BSL3 PFGE equipment or a documented procedure that ensures that all viable bacteria are inactivated is required.
Tamara, Revazishvili, Judith A, Johnson
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Pulsed-Field Gel Electrophoresis for Listeria monocytogenes
2015Pulsed-Field Gel Electrophoresis (PFGE) is a molecular subtyping method with high discriminatory power, reproducibility, and epidemiological concordance for the subtyping of Listeria monocytogenes and other bacteria. PFGE uses rare-cutting restriction enzymes (macrorestriction) that cut the genomic DNA, usually resulting in 6-25 DNA fragments ranging ...
Laura, Luque-Sastre +2 more
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Pulsed-Field Gel Electrophoresis of Pseudomonas aeruginosa
2015Pulsed-field gel electrophoresis (PFGE) is a technique used for the separation of high molecular weight restriction fragments from digested bacterial genomic DNA on a gel matrix by applying an electric field that periodically changes direction. PFGE has been shown to be the most discriminatory and reproducible bacterial strain typing technique ...
Honghua, Hu, Jim, Manos
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