Results 51 to 60 of about 146,626 (295)
Feature purify: An examination of spurious correlations in high-entropy alloys
Materials & DesignPatterns in datasets, while key to successful predictions, can also be misleading. In this study, a feature filtering workflow based on interpretable dimensionality reduction techniques was developed to diagnose the spurious correlations in high-entropy ...
Yue Pan +3 more
doaj +1 more source
Calpain small subunit homodimerization is robust and calcium‐independent
FEBS Letters, EarlyView.Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy +4 more
wiley +1 more source
Utilizing native ligands to purify membrane protein complexes in a soluble state
, 2022 Purification and characterization of membrane proteins is often limited by the ability to reconstitute membrane proteins into a soluble state with a membrane mimetic, while ensuring the protein is in a native state.
Wason, Irvinder Singh
core +1 more source
Single and Combined Methods to Specifically or Bulk-Purify RNA–Protein Complexes
Biomolecules, 2020 The ribonome interconnects the proteome and the transcriptome. Specific biology is situated at this interface, which can be studied in bulk using omics approaches or specifically by targeting an individual protein or RNA species. In this review, we focus
Roosje Van Ende +2 more
doaj +1 more source
Structural insights into an engineered feruloyl esterase with improved MHET degrading properties
FEBS Letters, EarlyView.A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa +5 more
wiley +1 more source
FEBS Letters, EarlyView.Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura +2 more
wiley +1 more source
Properties That Were First Used to Distinguish and Purify Dendritic Cells From Mouse Spleen
, 2002 Slide 9-19: Properties that were first used to distinguish and purify dendritic cells from mouse spleenhttps://digitalcommons.rockefeller.edu/diagrams/1018/thumbnail ...
Laboratory, Steinman
core +1 more source
Hypoosmotic Exposure of Canine Pancreatic Digest as a Means to Purify Islet Tissue
Cell Transplantation, 1997 The development of more effective means to separate pancreatic islets from the unwanted exocrine tissue would greatly advance the field of clinical islet allotransplantation in the treatment of insulin-dependent diabetes mellitus. Recent experiments with
Jonathan R.T. Lakey Ph.D. +4 more
doaj +1 more source
BMC Biotechnology, 2012 Background Many branches of biomedical research find use for pure recombinant proteins for direct application or to study other molecules and pathways.
Buhrman Jason S +3 more
doaj +1 more source
Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation
FEBS Letters, EarlyView.Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz +11 more
wiley +1 more source