Results 1 to 10 of about 883 (138)

Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase [PDF]

Microbial Cell Factories, 2017
Background Pyruvate decarboxylase (PDC) is a well-known pathway for ethanol production, but has not been demonstrated for high titer ethanol production at temperatures above 50 °C. Result Here we examined the thermostability of eight PDCs.
Liang Tian, Skyler J. Perot, Shuen Hon, Jilai Zhou, Xiaoyu Liang, Jason T. Bouvier, Adam M. Guss, Daniel G. Olson, Lee R. Lynd   +8 more
doaj   +2 more sources

Waterlogging Tolerance of Actinidia valvata Dunn Is Associated with High Activities of Pyruvate Decarboxylase, Alcohol Dehydrogenase and Antioxidant Enzymes [PDF]

Plants, 2023
Kiwifruit (Actinidia spp.) is susceptible to waterlogging stress. Although abundant wild germplasm resources exist among Actinidia plants for improving the waterlogging tolerance of kiwifruit cultivars, the underlying mechanisms remain largely unknown ...
Minxia Gao, Chaoyue Gai, Xinyu Li, Xin Feng, Ruilian Lai, Yuanyuan Song, Rensen Zeng, Daoqian Chen, Yiting Chen   +8 more
doaj   +2 more sources

Pyruvate decarboxylase and its lysine succinylation contribute to the development and metabolite biosynthesis in pathogenic fungus Aspergillus flavus

Mycology
This study evaluates the effects of pyruvate decarboxylase and its lysine succinylation (Ksucc) on the development of Aspergillus flavus and the production of secondary metabolites.
Guoqi Li, Elisabeth Tumukunde, Yuanyuan Chen, Yao Zhang, Yunlu Kuang, John Adejor, Meifang Wen, Shihua Wang   +7 more
doaj   +2 more sources

The effect of modulating the quantity of enzymes in a model ethanol pathway on metabolic flux in Synechocystis sp. PCC 6803 [PDF]

PeerJ, 2019
Synthetic metabolism allows new metabolic capabilities to be introduced into strains for biotechnology applications. Such engineered metabolic pathways are unlikely to function optimally as initially designed and native metabolism may not efficiently ...
Paulina Bartasun, Nicole Prandi, Marko Storch, Yarin Aknin, Mark Bennett, Arianna Palma, Geoff Baldwin, Yumiko Sakuragi, Patrik R. Jones, John Rowland   +9 more
doaj   +2 more sources

Redirection of the Reaction Specificity of a Thermophilic Acetolactate Synthase toward Acetaldehyde Formation. [PDF]

PLoS ONE, 2016
Acetolactate synthase and pyruvate decarboxylase are thiamine pyrophosphate-dependent enzymes that convert pyruvate into acetolactate and acetaldehyde, respectively.
Maria Cheng, Hayato Yoshiyasu, Kenji Okano, Hisao Ohtake, Kohsuke Honda   +4 more
doaj   +1 more source

The NADPH oxidase NOX4 regulates redox and metabolic homeostasis preventing HCC progression

Hepatology, EarlyView., 2022
Loss of NOX4 in HCC tumor cells induces metabolic reprogramming in a Nrf2/MYC‐dependent manner to promote HCC progression. Abstract Background and Aims The NADPH oxidase NOX4 plays a tumor‐suppressor function in HCC. Silencing NOX4 confers higher proliferative and migratory capacity to HCC cells and increases their in vivo tumorigenic potential in ...
Irene Peñuelas‐Haro, Rut Espinosa‐Sotelo, Eva Crosas‐Molist, Macarena Herranz‐Itúrbide, Daniel Caballero‐Díaz, Ania Alay, Xavier Solé, Emilio Ramos, Teresa Serrano, María L. Martínez‐Chantar, Ulla G. Knaus, José M. Cuezva, Antonio Zorzano, Esther Bertran, Isabel Fabregat   +14 more
wiley   +1 more source

Ethanologenesis and respiration in a pyruvate decarboxylase-deficient Zymomonas mobilis

BMC Research Notes, 2021
Objective Zymomonas mobilis is an alpha-proteobacterium with a rapid ethanologenic pathway, involving Entner–Doudoroff (E–D) glycolysis, pyruvate decarboxylase (Pdc) and two alcohol dehydrogenase (ADH) isoenzymes.
Reinis Rutkis, Inese Strazdina, Zane Lasa, Per Bruheim, Uldis Kalnenieks   +4 more
doaj   +1 more source

Rewiring carbon flow in Synechocystis PCC 6803 for a high rate of CO2-to-ethanol under an atmospheric environment

Frontiers in Microbiology, 2023
Cyanobacteria are an excellent microbial photosynthetic platform for sustainable carbon dioxide fixation. One bottleneck to limit its application is that the natural carbon flow pathway almost transfers CO2 to glycogen/biomass other than designed ...
E-Bin Gao, E-Bin Gao, Junhua Wu, Penglin Ye, Haiyan Qiu, Huayou Chen, Zhen Fang   +6 more
doaj   +1 more source

Validation of mathematical model with phosphate activation effect by batch (R)-phenylacetylcarbinol biotransformation process utilizing Candida tropicalis pyruvate decarboxylase in phosphate buffer

Scientific Reports, 2021
The (R)-phenylacetylcarbinol (PAC) batch biotransformation kinetics for partially purified Candida tropicalis TISTR 5350 pyruvate decarboxylase (PDC) were determined to validate a comprehensive mathematical model in 250 mL scale with 250 mM phosphate ...
Julaluk Khemacheewakul, Siraphat Taesuwan, Rojarej Nunta, Charin Techapun, Yuthana Phimolsiripol, Pornchai Rachtanapun, Kittisak Jantanasakulwong, Kritsadaporn Porninta, Sumeth Sommanee, Chatchadaporn Mahakuntha, Thanongsak Chaiyaso, Phisit Seesuriyachan, Alissara Reungsang, Ngoc Thao Ngan Trinh, Sutee Wangtueai, Sarana Rose Sommano, Noppol Leksawasdi   +16 more
doaj   +1 more source

Production of Phenylacetylcarbinol via Biotransformation Using the Co-Culture of Candida tropicalis TISTR 5306 and Saccharomyces cerevisiae TISTR 5606 as the Biocatalyst

Journal of Fungi, 2023
Phenylacetylcarbinol (PAC) is a precursor for the synthesis of several pharmaceuticals, including ephedrine, pseudoephedrine, and norephedrine. PAC is commonly produced through biotransformation using microbial pyruvate decarboxylase (PDC) in the form of
Anbarasu Kumar, Charin Techapun, Sumeth Sommanee, Chatchadaporn Mahakuntha, Juan Feng, Su Lwin Htike, Julaluk Khemacheewakul, Kritsadaporn Porninta, Yuthana Phimolsiripol, Wen Wang, Xinshu Zhuang, Wei Qi, Kittisak Jantanasakulwong, Rojarej Nunta, Noppol Leksawasdi   +14 more
doaj   +1 more source