The pyruvate decarboxylase activity of IpdC is a limitation for isobutanol production by Klebsiella pneumoniae [PDF]
Biotechnol Biofuels Bioprod, 2022 Background Klebsiella pneumoniae contains an endogenous isobutanol synthesis pathway. The ipdC gene annotated as an indole-3-pyruvate decarboxylase (Kp-IpdC), was identified to catalyze the formation of isobutyraldehyde from 2-ketoisovalerate.
Lin Shu +11 more
semanticscholar +5 more sources
Arabidopsis phenotyping reveals the importance of alcohol dehydrogenase and pyruvate decarboxylase for aerobic plant growth [PDF]
Scientific Reports, 2020 Alcohol dehydrogenase (ADH) and pyruvate decarboxylase (PDC) are key to the establishment of the fermentative metabolism in plants during oxygen shortage.
Irene Ventura +7 more
semanticscholar +3 more sources
Extreme anoxia tolerance in crucian carp and goldfish through neofunctionalization of duplicated genes creating a new ethanol-producing pyruvate decarboxylase pathway [PDF]
Scientific Reports, 2017 Without oxygen, most vertebrates die within minutes as they cannot meet cellular energy demands with anaerobic metabolism. However, fish of the genus Carassius (crucian carp and goldfish) have evolved a specialized metabolic system that allows them to ...
C. Fagernes +5 more
semanticscholar +3 more sources
Effect of Pyruvate Decarboxylase Knockout on Product Distribution Using Pichia pastoris (Komagataella phaffii) Engineered for Lactic Acid Production [PDF]
Bioengineering, 2018 Lactic acid is the monomer unit of the bioplastic poly-lactic acid (PLA). One candidate organism for lactic acid production is Pichia pastoris, a yeast widely used for heterologous protein production.
N. T. M. Melo +6 more
semanticscholar +4 more sources
Evaluation of pyruvate decarboxylase‐negative Saccharomyces cerevisiae strains for the production of succinic acid [PDF]
Eng Life Sci, 2019 Dicarboxylic acids are important bio‐based building blocks, and Saccharomyces cerevisiae is postulated to be an advantageous host for their fermentative production. Here, we engineered a pyruvate decarboxylase‐negative S.
A. Zahoor +3 more
semanticscholar +2 more sources
Enhanced production of 2,3-butanediol by engineered Saccharomyces cerevisiae through fine-tuning of pyruvate decarboxylase and NADH oxidase activities [PDF]
Biotechnol Biofuels, 2016 Background2,3-Butanediol (2,3-BD) is a promising compound for various applications in chemical, cosmetic, and agricultural industries. Pyruvate decarboxylase (Pdc)-deficient Saccharomyces cerevisiae is an attractive host strain for producing 2,3-BD ...
Jin-Woo Kim +5 more
semanticscholar +2 more sources
LIGHT-SABRE hyperpolarizes 1-^{13}C-pyruvate continuously, without magnetic field cycling [PDF]
J. Phys. Chem. C 2023, 127, 14, 6744-6753, 2023 Nuclear spin hyperpolarization enables real-time observation of metabolism and intermolecular interactions in vivo. 1-13C-Pyruvate is the leading hyperpolarized tracer currently under evaluation in several clinical trials as a promising molecular imaging agent.
arxiv +1 more source
Effect of the hydrogenation solvent in the PHIP-SAH hyperpolarization of [1- 13 C]pyruvate [PDF]
, 2021 ParaHydrogen Induced Polarization-Side Arm Hydrogenation (PHIP-SAH) is an inexpensive tool to obtain hyperpolarized pyruvate (and other metabolites) that can be applied to in-vivo diagnostics, for the investigation of metabolic processes. This method is based on hydrogenation, using hydrogen enriched in the para-isomer, of unsaturated substrates ...
arxiv +1 more source
A Metabolite Specific 3D Stack-of-Spiral bSSFP Sequence for Improved Lactate Imaging in Hyperpolarized [1-$^{13}$C]Pyruvate Studies on a 3T Clinical Scanner [PDF]
Magn Reson Med. 2020; 84: 1113-1125, 2020 Purpose: The balanced steady-state free precession sequence has been previously explored to improve the efficient use of non-recoverable hyperpolarized $^{13}$C magnetization, but suffers from poor spectral selectivity and long acquisition time. The purpose of this study was to develop a novel metabolite-specific 3D bSSFP ("MS-3DSSFP") sequence with ...
arxiv +1 more source
Radical-free hyperpolarized MRI using endogenously-occurring pyruvate analogues and UV-induced nonpersistent radicals [PDF]
, 2020 It was demonstrated that nonpersistent radicals can be generated in frozen solutions of metabolites such as pyruvate by irradiation with ultraviolet (UV) light, enabling radical-free dissolution DNP. Although pyruvate is endogenous, an excess of additional pyruvate may perturb metabolic processes, making it potentially unsuitable as a polarizing agent ...
arxiv +1 more source