Results 121 to 130 of about 145,153 (320)

The CRISPR‐Cas9 knockout DDC SH‐SY5Y in vitro model for AADC deficiency provides insight into the pathogenicity of R347Q and L353P variants: a cross‐sectional structural and functional analysis

open access: yesThe FEBS Journal, EarlyView.
Here, we generated a DDC‐KO cellular model for studying aromatic amino acid decarboxylase (AADC) deficiency. This model was created using CRISPR‐Cas9 in the SH‐SY5Y neuroblastoma cell line and was validated through the absence of the dopamine product and an altered l‐dopa metabolite profile.
Cristian Andres Carmona‐Carmona   +11 more
wiley   +1 more source

Computational, structural, and kinetic evidence that Vibrio vulnificus FrsA is not a cofactor-independent pyruvate decarboxylase.

open access: yesBiochemistry, 2013
The fermentation-respiration switch (FrsA) protein in Vibrio vulnificus was recently reported to catalyze the cofactor-independent decarboxylation of pyruvate. We now report quantum mechanical/molecular mechenical calculations that examine the energetics
Whitney F. Kellett   +7 more
semanticscholar   +1 more source

Remodeling of the terpenoid metabolism during prolonged phosphate depletion in the marine diatom Phaeodactylum tricornutum

open access: yesJournal of Phycology, EarlyView.
Abstract Terpenoids are a diverse class of naturally occurring organic compounds, which derive from five‐carbon isoprene units and play crucial roles in physiology, ecological interactions such as defense mechanisms, or adaptation to environmental stresses.
Florian Pruckner   +3 more
wiley   +1 more source

Quantitative model for $^{13}$C tracing applied to citrate production and secretion of prostate epithelial tissue [PDF]

open access: yesarXiv, 2019
Healthy human prostate epithelial cells have the unique ability to produce and secrete large amounts of citrate into the lumen of the prostate. Citrate is a Krebs cycle metabolite produced in the condensation reaction between acetyl-CoA and oxaloacetate in the mitochondria of the cell. With the application of $^{13}$C enriched substrates, such as $^{13}
arxiv  

Starmerella bombicola influences the metabolism of Saccharomyces cerevisiae at pyruvate decarboxylase and alcohol dehydrogenase level during mixed wine fermentation

open access: yesMicrobial Cell Factories, 2012
BackgroundThe use of a multistarter fermentation process with Saccharomyces cerevisiae and non-Saccharomyces wine yeasts has been proposed to simulate natural must fermentation and to confer greater complexity and specificity to wine.
V. Milanović   +3 more
semanticscholar   +1 more source

Comparative proteomics analysis of root and nodule mitochondria of soybean

open access: yesPlant, Cell &Environment, EarlyView.
Abstract Legumes perform symbiotic nitrogen fixation through rhizobial bacteroids housed in specialised root nodules. The biochemical process is energy‐intensive and consumes a huge carbon source to generate sufficient reducing power. To maintain the symbiosis, malate is supplied by legume nodules to bacteroids as their major carbon and energy source ...
Wai‐Ching Sin   +4 more
wiley   +1 more source

Dependence of Substrate Irradiation Reaction Rate Stimulation on Lactic Dehydrogenase Source [PDF]

open access: yesarXiv, 2013
Stimulation of LDH initial reaction rates by timed pre-irradiation of crystalline sodium pyruvate and lithium lactate is reported for enzymes isolated from rabbit muscle, pig heart, human erythrocytes and chicken heart. The phenomenon investigated is referred to as the Comorosan effect.
arxiv  

Bifunctionality of the thiamin diphosphate cofactor: assignment of tautomeric/ionization states of the 4'-aminopyrimidine ring when various intermediates occupy the active sites during the catalysis of yeast pyruvate decarboxylase.

open access: yesJournal of the American Chemical Society, 2012
Thiamin diphosphate (ThDP) dependent enzymes perform crucial C-C bond forming and breaking reactions in sugar and amino acid metabolism and in biosynthetic pathways via a sequence of ThDP-bound covalent intermediates.
Anand Balakrishnan   +5 more
semanticscholar   +1 more source

An Adaptation To Life In Acid Through A Novel Mevalonate Pathway. [PDF]

open access: yes, 2016
Extreme acidophiles are capable of growth at pH values near zero. Sustaining life in acidic environments requires extensive adaptations of membranes, proton pumps, and DNA repair mechanisms.
Bowie, James U   +3 more
core   +1 more source

Home - About - Disclaimer - Privacy