Results 11 to 20 of about 19,748 (208)

Validation of mathematical model with phosphate activation effect by batch (R)-phenylacetylcarbinol biotransformation process utilizing Candida tropicalis pyruvate decarboxylase in phosphate buffer [PDF]

open access: yesScientific Reports, 2021
The (R)-phenylacetylcarbinol (PAC) batch biotransformation kinetics for partially purified Candida tropicalis TISTR 5350 pyruvate decarboxylase (PDC) were determined to validate a comprehensive mathematical model in 250 mL scale with 250 mM phosphate ...
Julaluk Khemacheewakul   +16 more
doaj   +2 more sources

Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase [PDF]

open access: yesMicrobial Cell Factories, 2017
Background Pyruvate decarboxylase (PDC) is a well-known pathway for ethanol production, but has not been demonstrated for high titer ethanol production at temperatures above 50 °C. Result Here we examined the thermostability of eight PDCs.
Liang Tian   +8 more
doaj   +2 more sources

The Bifunctional Pyruvate Decarboxylase/Pyruvate Ferredoxin Oxidoreductase fromThermococcus guaymasensis [PDF]

open access: yesArchaea, 2014
The hyperthermophilic archaeonThermococcus guaymasensisproduces ethanol as a metabolic end product, and an alcohol dehydrogenase (ADH) catalyzing the reduction of acetaldehyde to ethanol has been purified and characterized. However, the enzyme catalyzing the formation of acetaldehyde has not been identified.
Mohammad S. Eram   +2 more
openaire   +4 more sources

Translocation of Zymomonas mobilis pyruvate decarboxylase to periplasmic compartment for production of acetaldehyde outside the cytosol [PDF]

open access: yesMicrobiologyOpen, 2019
Acetaldehyde, a valuable commodity chemical, is a volatile inhibitory byproduct of aerobic fermentation in Zymomonas mobilis and in several other microorganisms.
Elina Balodite   +6 more
doaj   +2 more sources

Role of phosphate limitation and pyruvate decarboxylase in rewiring of the metabolic network for increasing flux towards isoprenoid pathway in a TATA binding protein mutant of Saccharomyces cerevisiae [PDF]

open access: yesMicrobial Cell Factories, 2018
Background Production of isoprenoids, a large and diverse class of commercially important chemicals, can be achieved through engineering metabolism in microorganisms. Several attempts have been made to reroute metabolic flux towards isoprenoid pathway in
Manisha Wadhwa   +3 more
doaj   +2 more sources

Extreme anoxia tolerance in crucian carp and goldfish through neofunctionalization of duplicated genes creating a new ethanol-producing pyruvate decarboxylase pathway [PDF]

open access: yesScientific Reports, 2017
Without oxygen, most vertebrates die within minutes as they cannot meet cellular energy demands with anaerobic metabolism. However, fish of the genus Carassius (crucian carp and goldfish) have evolved a specialized metabolic system that allows them to ...
Cathrine E. Fagernes   +5 more
doaj   +2 more sources

Arabidopsis phenotyping reveals the importance of alcohol dehydrogenase and pyruvate decarboxylase for aerobic plant growth [PDF]

open access: yesScientific Reports, 2020
Alcohol dehydrogenase (ADH) and pyruvate decarboxylase (PDC) are key to the establishment of the fermentative metabolism in plants during oxygen shortage.
Irene Ventura   +7 more
doaj   +2 more sources

High yield production of 3-hydroxypropionic acid using Issatchenkia orientalis [PDF]

open access: yesNature Communications
Biomanufacturing provides a more sustainable alternative to fossil-based chemical manufacturing. 3-Hydroxypropionic acid (3HP) is a top Department of Energy value-added chemical and precursor to bioplastics, yet cost-effective microbial production ...
Shih-I Tan   +9 more
doaj   +2 more sources

Effect of Pyruvate Decarboxylase Knockout on Product Distribution Using Pichia pastoris (Komagataella phaffii) Engineered for Lactic Acid Production [PDF]

open access: yesBioengineering, 2018
Lactic acid is the monomer unit of the bioplastic poly-lactic acid (PLA). One candidate organism for lactic acid production is Pichia pastoris, a yeast widely used for heterologous protein production.
Nadiele T. M. Melo   +6 more
doaj   +2 more sources

Zymobacter palmae Pyruvate Decarboxylase is Less Effective Than That of Zymomonas mobilis for Ethanol Production in Metabolically Engineered Synechocystis sp. PCC6803 [PDF]

open access: yesMicroorganisms, 2019
To produce bioethanol from model cyanobacteria such as Synechocystis, a two gene cassette consisting of genes encoding pyruvate decarboxylase (PDC) and alcohol dehydrogenase (ADH) are required to transform pyruvate first to acetaldehyde and then to ...
Lorraine Quinn   +5 more
doaj   +2 more sources

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