Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase [PDF]
Microbial Cell Factories, 2017 BackgroundPyruvate decarboxylase (PDC) is a well-known pathway for ethanol production, but has not been demonstrated for high titer ethanol production at temperatures above 50 °C.ResultHere we examined the thermostability of eight PDCs.
Liang Tian +8 more
semanticscholar +6 more sources
Ethanologenesis and respiration in a pyruvate decarboxylase-deficient Zymomonas mobilis [PDF]
BMC Research Notes, 2021 Objective Zymomonas mobilis is an alpha-proteobacterium with a rapid ethanologenic pathway, involving Entner–Doudoroff (E–D) glycolysis, pyruvate decarboxylase (Pdc) and two alcohol dehydrogenase (ADH) isoenzymes.
Reinis Rutkis +4 more
doaj +6 more sources
Waterlogging Tolerance of Actinidia valvata Dunn Is Associated with High Activities of Pyruvate Decarboxylase, Alcohol Dehydrogenase and Antioxidant Enzymes [PDF]
Plants, 2023 Kiwifruit (Actinidia spp.) is susceptible to waterlogging stress. Although abundant wild germplasm resources exist among Actinidia plants for improving the waterlogging tolerance of kiwifruit cultivars, the underlying mechanisms remain largely unknown ...
Minxia Gao +8 more
doaj +4 more sources
The Bifunctional Pyruvate Decarboxylase/Pyruvate Ferredoxin Oxidoreductase from Thermococcus guaymasensis [PDF]
Archaea, 2014 The hyperthermophilic archaeon Thermococcus guaymasensis produces ethanol as a metabolic end product, and an alcohol dehydrogenase (ADH) catalyzing the reduction of acetaldehyde to ethanol has been purified and characterized.
M. Eram, Erica Oduaran, K. Ma
semanticscholar +6 more sources
Structure and functional characterization of pyruvate decarboxylase from Gluconacetobacter diazotrophicus [PDF]
BMC Struct Biol, 2014 BackgroundBacterial pyruvate decarboxylases (PDC) are rare. Their role in ethanol production and in bacterially mediated ethanologenic processes has, however, ensured a continued and growing interest.
L. V. van Zyl +3 more
semanticscholar +6 more sources
Analysis of the role of the pyruvate decarboxylase gene family in Arabidopsis thaliana under low-oxygen conditions. [PDF]
Plant biology, 2014 Plants under low-oxygen conditions adapt their metabolism by inducing the fermentative pathway, with ethanol as the predominant end product. Activities of pyruvate decarboxylase (PDC) and alcohol dehydrogenase (ADH) are required for this pathway. While a
M. Mithran +4 more
semanticscholar +4 more sources
Validation of mathematical model with phosphate activation effect by batch (R)-phenylacetylcarbinol biotransformation process utilizing Candida tropicalis pyruvate decarboxylase in phosphate buffer [PDF]
Scientific Reports, 2021 The (R)-phenylacetylcarbinol (PAC) batch biotransformation kinetics for partially purified Candida tropicalis TISTR 5350 pyruvate decarboxylase (PDC) were determined to validate a comprehensive mathematical model in 250 mL scale with 250 mM phosphate ...
Julaluk Khemacheewakul +16 more
doaj +2 more sources
The Effect of myo-Inositol on Pyruvate Decarboxylase
THE JOURNAL OF VITAMINOLOGY, 1969 Saccharomyces carlsbergensis 4228 (ATCC 9080) was cultivated for 24 hours in four different media containing 0, 50, 150 and 500μg of myo-inositol per 20ml. The growth of the yeast and the specific activity of pyruvate decarboxylase extracted from the respective cell preparations were found to be proportional to the amount of inositol in the medium. The
Takako Tomita, Isao Tomita, T. Ozawa
openalex +5 more sources
Pyruvate decarboxylase activity of the acetohydroxyacid synthase of Thermotoga maritima [PDF]
Biochem Biophys Rep, 2016 Acetohydroxyacid synthase (AHAS) catalyzes the production of acetolactate from pyruvate. The enzyme from the hyperthermophilic bacterium Thermotoga maritima has been purified and characterized (kcat ~100 s-1). It was found that the same enzyme also had the ability to catalyze the production of acetaldehyde and CO2 from pyruvate, an activity of pyruvate
M. Eram, K. Ma
semanticscholar +5 more sources
Translocation of Zymomonas mobilis pyruvate decarboxylase to periplasmic compartment for production of acetaldehyde outside the cytosol [PDF]
MicrobiologyOpen, 2019 Acetaldehyde, a valuable commodity chemical, is a volatile inhibitory byproduct of aerobic fermentation in Zymomonas mobilis and in several other microorganisms.
Elina Balodite +6 more
doaj +2 more sources