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Hypoxic pregnancy promotes fibrosis and increases stress metabolites in the ovine fetal liver. [PDF]

open access: yesJ Physiol
McGuckin MM   +8 more
europepmc   +1 more source

One-Step Purification of the Recombinant Catalytic Subunit of Pyruvate Dehydrogenase Phosphatase

open access: closedProtein Expression and Purification, 2000
A facile one-step affinity chromatographic purification of the recombinant catalytic subunit (PDPc) of bovine pyruvate dehydrogenase phosphatase (PDP) to near homogeneity is described. PDPc binds in the presence of Ca(2+) to the inner lipoyl domain (L2) of the dihydrolipoamide acetyltransferase component (E2) of the mammalian pyruvate dehydrogenase ...
Wahn Soo Choi   +4 more
openalex   +4 more sources
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Inhibition of pyruvate dehydrogenase and pyruvate dehydrogenase phosphate phosphatase by glyoxylate

Bioorganic Chemistry, 1985
Abstract The overall reaction catalyzed by the pyruvate dehydrogenase complex from rat epididymal fat tissue is inhibited by glyoxylate at concentrations greater than 10 μ m . The inhibition is competitive with respect to pyruvate; Ki was found to be 80 μ m .
Gordon A. Hamilton, Susan M. Beatty
openaire   +2 more sources

Stimulation of pyruvate dehydrogenase phosphatase activity by polyamines

Biochemical and Biophysical Research Communications, 1984
Pyruvate dehydrogenase phosphatase requires Mg2+ or Mn2+, and its activity in the presence of Mg2+ is markedly stimulated by Ca2+. At saturating Mg2+ and Ca2+ concentrations, the polyamines spermine, spermidine and putrescine stimulated the activity of pyruvate dehydrogenase phosphatase 1.5- to 3-fold. Spermine was the most active of the polyamines. At
Lester J. Reed   +2 more
openaire   +3 more sources

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