Results 221 to 230 of about 623,101 (265)

Development and validation of a quantitative pcr array assay for the detection of viral sequences in breast cancer. [PDF]

J Genet Eng Biotechnol
Del Carmen Trujillo-Murillo K, Lugo-Trampe A, Rodríguez-Sánchez IP, Martínez-Fierro ML, Cordero-Chaclan C, Tafurt-Cardona Y, Pérez PM, de Jesús Joo-Domínguez A, De la Cruz-Calderón R, Espinoza-Ruiz M, Chang-Rueda C, Lee-Faviel FC.   +11 more
europepmc   +1 more source

m6A‐Mediated Glycolysis by IL‐37 Drives T Cell Metabolic Reprogramming to Regulate Colitis

Advanced Science, EarlyView.
This study identifies an IL‐37/SIGIRR‐METTL14 regulatory axis that suppresses global m6A modification in CD4+ T cells. IL‐37 signaling, mediated through SIGIRR, inhibits IRAK4 and JNK phosphorylation, leading to downregulation of the methyltransferase METTL14.
Xiaoyan Wang, Jiadong Yu, Guolin Li, Ya Li, Fanlian Zeng, Jing Hu, Yifan Zhou, Chengcheng Yue, Qixiang Zhao, Chen Zhang, Hong Zhou, Fulei Zhao, Yawen Hu, Pei Zhou, Linna Gu, Yuting Feng, Mingxiang He, Shishi Huang, Xiaochi Sun, Xinmeng Wang, Jie Tang, Xinyu Zeng, Wenling Wu, Nongyu Huang, Xinai Cui, Kaijun Cui, Jiong Li   +26 more
wiley   +1 more source

Transcriptional capacity limits copper resistance in yeast harboring highly expanded <i>CUP1</i> arrays. [PDF]

MicroPubl Biol
Takesue H, Okada S, Ito T.
europepmc   +1 more source

RT-qPCR v1

, 2020
openaire   +1 more source

Selection and Validation of Stable Reference Genes for RT-qPCR in <i>Diaphorencyrtus aligarhensis</i>-A Predominant Parasitoid of <i>Diaphorina citri</i>. [PDF]

Int J Mol Sci
Gu X, Luo B, Zhang S, Chen J, Xu P, Li S, Qiu B, Zhang L.   +7 more
europepmc   +1 more source

Mapping the metagenomic landscape: combined shotgun sequencing and quantitative PCR to profile gut metagenome-assembled genomes in marmosets following treatment with a broad-spectrum antibiotic cocktail. [PDF]

Gut Microbes
Hernandez JB, Abiodun M, Hayer SS, Dickson T, Ayayee P, Clayton JB.   +5 more
europepmc   +1 more source

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MNAzyme qPCR: A Superior Tool for Multiplex qPCR

2013
Multicomponent nucleic acid enzymes (MNAzymes) are nucleic acid enzymes composed of multiple oligonucleotide partzymes that only associate to form catalytic complexes in the presence of a target nucleic acid. Once assembled, MNAzymes cleave a separate substrate (probe) between fluorophore and quencher labels to produce a fluorescent signal indicative ...
Elisa, Mokany, Alison V, Todd
openaire   +2 more sources

qPCR Genotyping of Polyploid Species

2023
A simple and cost-effective method for genotyping polyploid plants using quantitative PCR (qPCR) is described in this chapter. There is no additional operation, only simultaneous amplification of alleles and reference sequences with constant copy number in the genome.
Haiyan, Wang, Jiangbo, Dang, Qigao, Guo, Guolu, Liang   +3 more
openaire   +2 more sources

How to Combine ChIP with qPCR

2017
Chromatin immunoprecipitation (ChIP) coupled with quantitative PCR (qPCR) has in the last 15 years become a basic mainstream tool in genomic research. Numerous commercially available ChIP kits, qPCR kits, and real-time PCR systems allow for quick and easy analysis of virtually anything chromatin-related as long as there is an available antibody ...
openaire   +2 more sources

qPCR-MasterMix (qPCR-MM)

Cold Spring Harbor Protocols, 2023
openaire   +1 more source