Results 231 to 240 of about 619,072 (268)

qPCR Genotyping of Polyploid Species

2023
A simple and cost-effective method for genotyping polyploid plants using quantitative PCR (qPCR) is described in this chapter. There is no additional operation, only simultaneous amplification of alleles and reference sequences with constant copy number in the genome.
Haiyan, Wang   +3 more
openaire   +2 more sources

MNAzyme qPCR: A Superior Tool for Multiplex qPCR

2013
Multicomponent nucleic acid enzymes (MNAzymes) are nucleic acid enzymes composed of multiple oligonucleotide partzymes that only associate to form catalytic complexes in the presence of a target nucleic acid. Once assembled, MNAzymes cleave a separate substrate (probe) between fluorophore and quencher labels to produce a fluorescent signal indicative ...
Elisa, Mokany, Alison V, Todd
openaire   +2 more sources

How to Combine ChIP with qPCR

2017
Chromatin immunoprecipitation (ChIP) coupled with quantitative PCR (qPCR) has in the last 15 years become a basic mainstream tool in genomic research. Numerous commercially available ChIP kits, qPCR kits, and real-time PCR systems allow for quick and easy analysis of virtually anything chromatin-related as long as there is an available antibody ...
openaire   +2 more sources

eDNA-qPCR-Rockies

The objectif is to link eDNA concentration to manipulated brook trout density from the Rockies. This repository contains all the data and codes used for the publication.
Beaulieu, Julien   +4 more
openaire   +1 more source

qPCR-MasterMix (qPCR-MM)

Cold Spring Harbor Protocols, 2023
openaire   +1 more source

QPCR without the 'P'

Nature Methods, 2009
With the addition of a ligand-sensing aptamer sequence, a self-replicating RNA enzyme system enables general molecular detection, analogous to that of quantitative PCR.
openaire   +1 more source

DNA extraction leads to bias in bacterial quantification by qPCR

Applied Microbiology and Biotechnology, 2022
Angela Lima   +2 more
exaly  

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