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Comparative Analysis of Full-Length Reference Gene Stability in <i>Phoebe zhennan</i> Under Primary Abiotic and Biotic Stresses. [PDF]
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qPCR Genotyping of Polyploid Species
2023A simple and cost-effective method for genotyping polyploid plants using quantitative PCR (qPCR) is described in this chapter. There is no additional operation, only simultaneous amplification of alleles and reference sequences with constant copy number in the genome.
Haiyan, Wang +3 more
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MNAzyme qPCR: A Superior Tool for Multiplex qPCR
2013Multicomponent nucleic acid enzymes (MNAzymes) are nucleic acid enzymes composed of multiple oligonucleotide partzymes that only associate to form catalytic complexes in the presence of a target nucleic acid. Once assembled, MNAzymes cleave a separate substrate (probe) between fluorophore and quencher labels to produce a fluorescent signal indicative ...
Elisa, Mokany, Alison V, Todd
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2017
Chromatin immunoprecipitation (ChIP) coupled with quantitative PCR (qPCR) has in the last 15 years become a basic mainstream tool in genomic research. Numerous commercially available ChIP kits, qPCR kits, and real-time PCR systems allow for quick and easy analysis of virtually anything chromatin-related as long as there is an available antibody ...
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Chromatin immunoprecipitation (ChIP) coupled with quantitative PCR (qPCR) has in the last 15 years become a basic mainstream tool in genomic research. Numerous commercially available ChIP kits, qPCR kits, and real-time PCR systems allow for quick and easy analysis of virtually anything chromatin-related as long as there is an available antibody ...
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The objectif is to link eDNA concentration to manipulated brook trout density from the Rockies. This repository contains all the data and codes used for the publication.
Beaulieu, Julien +4 more
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Beaulieu, Julien +4 more
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Nature Methods, 2009
With the addition of a ligand-sensing aptamer sequence, a self-replicating RNA enzyme system enables general molecular detection, analogous to that of quantitative PCR.
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With the addition of a ligand-sensing aptamer sequence, a self-replicating RNA enzyme system enables general molecular detection, analogous to that of quantitative PCR.
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DNA extraction leads to bias in bacterial quantification by qPCR
Applied Microbiology and Biotechnology, 2022Angela Lima +2 more
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