Results 211 to 220 of about 167,371,403 (279)

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Impact of depressive symptoms in social functioning and quality of life in Brazilian primary care service users

, 2001
Grupo LIDO (Longitudinal Investigation of Depression Outcomes), Lima, Ana Flavia Barros da Silva, Schestatsky, Gustavo, Borges, Vivian Roxo, Camey, Suzi Alves, Fleck, Marcelo Pio de Almeida, Louzada, Sérgio Noll, Henriques, Alexandre Annes   +7 more
core   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Effects of the COVID-19 pandemic on working conditions and mental health among employees in health and social services. [PDF]

Front Public Health
Peters C, Dulon M, Kersten JF, Hartung C, Nienhaus A, Schablon A.   +5 more
europepmc   +1 more source

Work Stress, Quality of Work Life, and Burnout Among Registered Nurses in Public Hospitals in Samut Songkhram Province, Thailand: An Explanatory Sequential Mixed-Methods Study. [PDF]

Int J Environ Res Public Health
Choompunuch B, Sani E, Sillabutra J, Ounprasertsuk J.   +3 more
europepmc   +1 more source

Women's Work and its Effects on Personal Wellbeing and Satisfaction with Life: A Cross-Sectional Comparative Study. [PDF]

Med Lav
Mohammed H, Awaad A, El-Gilany AH, Abdelraouf S, El-Refaay E.   +4 more
europepmc   +1 more source