Results 21 to 30 of about 2,099,372 (258)

Quantitative Mass Spectrometry-Based Proteomics: An Overview.

Methods in molecular biology, 2021
In recent decades, mass spectrometry has moved more than ever before into the front line of protein-centered research. After being established at the qualitative level, the more challenging question of quantification of proteins and peptides using mass ...
S. Rozanova, Katalin Barkovits, Miroslav Nikolov, Carla Schmidt, H. Urlaub, K. Marcus   +5 more
semanticscholar   +1 more source

The Quantitative Methods Boot Camp: Teaching Quantitative Thinking and Computing Skills to Graduate Students in the Life Sciences

PLoS Comput. Biol., 2015
The past decade has seen a rapid increase in the ability of biologists to collect large amounts of data. It is therefore vital that research biologists acquire the necessary skills during their training to visualize, analyze, and interpret such data.
Melanie I. Stefan, Johanna L. Gutlerner, R. Born, Michael Springer   +3 more
semanticscholar   +1 more source

A Novel Systems-Biology Algorithm for the Analysis of Coordinated Protein Responses Using Quantitative Proteomics*

Molecular & Cellular Proteomics, 2016
The coordinated behavior of proteins is central to systems biology. However, the underlying mechanisms are poorly known and methods to analyze coordination by conventional quantitative proteomics are still lacking. We present the Systems Biology Triangle
F. Garcia-Marques, Marco Trevisan-Herraz, S. Martínez-Martínez, E. Camafeita, I. Jorge, J. López, N. Méndez-Barbero, S. Méndez-Ferrer, M. A. del Pozo, B. Ibáñez, V. Andrés, F. Sánchez‐Madrid, J. Redondo, Elena Bonzón-Kulichenko, Jesús Vázquez   +14 more
semanticscholar   +1 more source

Characterizing Parental Concerns About Lasting Impacts of Treatment in Children With B‐Acute Lymphoblastic Leukemia

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Background B‐acute lymphoblastic leukemia (B‐ALL) is the most common pediatric cancer, and while most children in high‐resource settings are cured, therapy carries risks for long‐term toxicities. Understanding parents’ concerns about these late effects is essential to guide anticipatory support and inform evolving therapeutic approaches ...
Kellee N. Parker, Sarah W. Alexander, Lisa M. Jacola, Kimberly A. Buff, Kyobin Hwang, Elham Hashemi, Gregory Stoddard, Lindsay A. Jibb   +7 more
wiley   +1 more source

Data‐independent acquisition‐based SWATH‐MS for quantitative proteomics: a tutorial

Molecular Systems Biology, 2018
Many research questions in fields such as personalized medicine, drug screens or systems biology depend on obtaining consistent and quantitatively accurate proteomics data from many samples. SWATH‐MS is a specific variant of data‐independent acquisition (
C. Ludwig, Ludovic C. Gillet, George A. Rosenberger, S. Amon, Ben C. Collins, R. Aebersold   +5 more
semanticscholar   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Quantitative biology: where modern biology meets physical sciences

Molecular Biology of the Cell, 2014
Quantitative methods and approaches have been playing an increasingly important role in cell biology in recent years. They involve making accurate measurements to test a predefined hypothesis in order to compare experimental data with predictions ...
S. Shekhar, Lian Zhu, L. Mazutis, A. E. Sgro, Thomas G. Fai, Marija Podolski   +5 more
semanticscholar   +1 more source

The Effect of Blended Project-based Learning Integrated with 21st-Century Skills on Pre-Service Biology Teachers' Higher-order Thinking Skills

Jurnal Pendidikan IPA Indonesia, 2022
Educational institutions are required to prepare competent and competitive pre-service teacher graduates who have skills according to the needs of the 21st century.
N. Hujjatusnaini, A. Corebima, S. Prawiro, A. Gofur   +3 more
semanticscholar   +1 more source

The Caenorhabditis elegans DPF‐3 and human DPP4 have tripeptidyl peptidase activity

FEBS Letters, EarlyView.
The dipeptidyl peptidase IV (DPPIV) family comprises serine proteases classically defined by their ability to remove dipeptides from the N‐termini of substrates, a feature that gave the family its name. Here, we report the discovery of a previously unrecognized tripeptidyl peptidase activity in DPPIV family members from two different species.
Aditya Trivedi, Rajani Kanth Gudipati
wiley   +1 more source

Interplay between circadian and other transcription factors—Implications for cycling transcriptome reprogramming

FEBS Letters, EarlyView.
This perspective highlights emerging insights into how the circadian transcription factor CLOCK:BMAL1 regulates chromatin architecture, cooperates with other transcription factors, and coordinates enhancer dynamics. We propose an updated framework for how circadian transcription factors operate within dynamic and multifactorial chromatin landscapes ...
Xinyu Y. Nie, Jerome S. Menet
wiley   +1 more source