Results 81 to 90 of about 643,214 (199)

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Quantitative flow ratio and fractional flow reserve mismatch – clinical and biochemical predictors of measurement discrepancy

Advances in Interventional Cardiology, 2019
Martyna Zaleska, Lukasz Koltowski, Jakub Maksym, Aleksandra Chabior, Aleksandra Pohadajło, Mateusz Soliński, Mariusz Tomaniak, Grzegorz Opolski, Janusz Kochman   +8 more
doaj   +1 more source

Ultrasonic flow ratio measured immediately after successful rotational atherectomy with stent implantation predicts major adverse cardiovascular events

Frontiers in Cardiovascular Medicine
IntroductionThe potential role of post-percutaneous coronary intervention (PCI) quantitative flow ratio (QFR) and ultrasonic flow ratio (UFR) in predicting adverse outcomes in patients with successful rotational atherectomy (RA) and stent placement ...
Tao Zhao, Tao Zhao, Tao Zhao, Qing Jin, Qing Jin, Qing Jin, Xi Zhang, Xi Zhang, Xi Zhang, Jiaji He, Jiaji He, Jiaji He, Guiping He, Guiping He, Guiping He, Qiu Chen, Qiu Chen, Qiu Chen, Yikang Sun, Yikang Sun, Pin Gan, Pin Gan, Jilei Zhang, Jilei Zhang, Xuefeng Guang, Xuefeng Guang, Xuefeng Guang, Qiang Xue, Qiang Xue, Qiang Xue   +29 more
doaj   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

A Visualized Nomogram for Predicting Prognosis in Elderly Patients after Percutaneous Coronary Intervention

Reviews in Cardiovascular Medicine
Background: Revascularized patients still experience adverse cardiovascular events. This is particularly true for elderly patients over the age of 65, as they often have more co-morbid vascular conditions.
Qin Chen, Yuxiang Chen, Ruijin Hong, Jiaxin Zhong, Lihua Chen, Yuanming Yan, Lianglong Chen, Yukun Luo   +7 more
doaj   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Prognostic Value of Quantitative Flow Ratio Combined with SYNTAX Scores I/II in Multivessel Coronary Artery Disease: A Small-Sample, Single-Center Study

Reviews in Cardiovascular Medicine
Background: A fractional flow reserve (FFR)-fixed-SYNTAX score could decrease the number of high-risk patients. This study explored the prognostic value of non-invasive quantitative flow ratio (QFR)-fixed-SYNTAX I/II scores in ...
Shuyi Zhang, Ming Wang, Qian Gan, Xinrong Zhai, Yang Chen, Shaofeng Guan, Xinxin Xu, Jiasheng Wen, Xinkai Qu, Wenzheng Han   +9 more
doaj   +1 more source

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

FEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta, Sebastian GB Furness, Tahereh Gharbi, Ric De Paoli‐Iseppi, Shweta S. Joshi, Michael Clark, David L. Hare, Peter Wookey   +7 more
wiley   +1 more source