Results 191 to 200 of about 2,512,472 (268)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Correction: Glial cell proteome using targeted quantitative methods for potential multi-diagnostic biomarkers. [PDF]

Clin Proteomics
Kang N, Oh HJ, Hong JH, Moon HE, Kim Y, Lee HJ, Min H, Park H, Lee SH, Paek SH, Jin J.   +10 more
europepmc   +1 more source

Quantitative methods used to evaluate impact of health promotion interventions to prevent HIV infections: a methodological systematic review protocol. [PDF]

Syst Rev, 2022
Ravalihasy A, Kardaś-Słoma L, Yazdanpanah Y, Ridde V.   +3 more
europepmc   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Further Analysis of Advanced Quantitative Methods and Supplemental Interpretative Aids with Single-Case Experimental Designs. [PDF]

Perspect Behav Sci, 2022
Falligant JM, Kranak MP, Hagopian LP.
europepmc   +1 more source

SIRT4 positively regulates autophagy via ULK1, but independently of HDAC6 and OPA1

FEBS Open Bio, EarlyView.
Cells expressing SIRT4 (H161Y), a catalytically inactive mutant of the sirtuin SIRT4, fail to upregulate LC3B‐II and exhibit a reduced autophagic flux under stress conditions. Interestingly, SIRT4(H161Y) promotes phosphorylation of ULK1 at S638 and S758 that are associated with inhibition of autophagy initiation.
Isabell Lehmkuhl, Khawar Amin, Lydia Gabriel, Nils Hampel, Afshin Iram, Julia Hesse, Constanze Wiek, Jasmin Thuy Vy Nguyen, Helmut Hanenberg, Jürgen Scheller, M. Reza Ahmadian, Björn Stork, Doreen M. Floss, Roland P. Piekorz   +13 more
wiley   +1 more source

Comparative Analysis of Quantitative Methods for Campylobacter spp. Quantification: ISO 10272-2:2017, Tempo^® and Real-Time PCR in Refrigerated and Frozen Turkey Cuts. [PDF]

Foods
Führ CA, Giombelli A, Cerutti MF, Bergmann GP, Kindlein L.   +4 more
europepmc   +1 more source

Metformin promotes mitochondrial integrity through AMPK‐signaling in Leber's hereditary optic neuropathy

FEBS Open Bio, EarlyView.
Metformin mediates mitochondrial quality control in Leber's hereditary optic neuropathy (LHON) fibroblasts carrying mtDNA mutations. At therapeutic levels, metformin activates AMPK signaling to restore mitochondrial dynamics by promoting fusion and restraining fission, while preserving mitochondrial mass, enhancing autophagy/mitophagy and biogenesis ...
Chatnapa Panusatid, Rapasviranda Soiyangsuk, Maneeluck Tanadjindarat, Chayanon Peerapittayamongkol   +3 more
wiley   +1 more source

Special Mini-Issue: Quantitative methods to decipher cellular heterogeneity - from single-cell to spatial omic methods. [PDF]

Biophys Rev
Chen X, Wu AR.
europepmc   +1 more source