Evolution‐guided yeast complementation reveals functional differences in human PSPH variants
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez +6 more
wiley +1 more source
Lensless Quantitative Phase Imaging with Bayer-Filtered Color Sensors Under Sequential RGB-LED Illumination. [PDF]
Wu J, Li Y, Luo Y, Pan L, Song P, Xu Q.
europepmc +1 more source
Analysis of structural effects of sickle cell disease on brain vasculature of mice using three-dimensional quantitative phase imaging. [PDF]
Filan C, Song H, Platt MO, Robles FE.
europepmc +1 more source
Quantitative phase imaging for label‐free cytometry [PDF]
Elena, Holden +2 more
openaire +2 more sources
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Label-free in-line characterization of immune cell culture using quantitative phase imaging. [PDF]
Serafini CE +18 more
europepmc +1 more source
Polarization differential interference contrast microscopy with physics-inspired plug-and-play denoiser for single-shot high-performance quantitative phase imaging. [PDF]
Aleksandrovych M +3 more
europepmc +1 more source
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti +5 more
wiley +1 more source
Quantitative Phase Imaging Using Digital Holographic Microscopy to Assess the Degree of Intestinal Inflammation in Patients with Ulcerative Colitis. [PDF]
Bokemeyer A +9 more
europepmc +1 more source

