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GPC and quantitative phase imaging
SPIE Proceedings, 2016Generalized Phase Contrast (GPC) is a light efficient method for generating speckle-free contiguous optical distributions using binary-only or analog phase levels. It has been used in applications such as optical trapping and manipulation, active microscopy, structured illumination, optical security, parallel laser marking and labelling and recently in
Darwin Palima +3 more
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Quantitative phase imaging in biomedicine
Nature Photonics, 2018Quantitative phase imaging (QPI) has emerged as a valuable method for investigating cells and tissues. QPI operates on unlabelled specimens and, as such, is complementary to established fluorescence microscopy, exhibiting lower phototoxicity and no photobleaching. As the images represent quantitative maps of optical path length delays introduced by the
YongKeun Park +2 more
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Quantitative Phase Imaging by Evanescent Wave Microscopy
Frontiers in Optics 2017, 2017Here we show the versatility of Digital Holography Microscopy for the development of innovative systems for quantitative phase imaging of Total Internal Reflection.
Mandracchia B, Paturzo M, Ferraro P
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Quantitative Phase Imaging in Microscopy
2009Phase imaging can be used in a reflection or transmission geometry. In biology it has the advantage of being marker-free. It can measure physical shape, and refractive index variations, ideally in three dimensions (3D). In transmission, phase is related to optical path difference.
Colin JR Sheppard +2 more
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Coded aperture pair for quantitative phase imaging
Optics Letters, 2014This Letter proposes a novel quantitative phase-imaging approach by optically encoding light fields into a complementary image pair followed by computational reconstruction. We demonstrate that the axial intensity derivative for phase recovery can be well estimated by a coded-aperture image pair without z axial scanning.
Jiamin, Wu +4 more
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Quantitative phase imaging by optimized asymmetric illumination
Applied Optics, 2017We have presented a simple approach for quantitative phase imaging by optimizing asymmetric illumination of a conventional microscope. With this illumination, the light intensity modulation accompanying refraction at the surface profile of phase objects occurs, and "phase-gradient information" can be derived by detecting it.
Yoshimasa, Suzuki +3 more
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Autofocus in quantitative phase imaging
Unconventional Optical Imaging III, 2022Yannuo Wen, Yue Wang, John J. Healy
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Superphantoms for Quantitative Phase Imaging
Optica Imaging Congress 2025 (3D, DH, COSI, IS, pcAOP,RadIT)Superphantoms are crafted from imaging and functional data to replicate optical and structural properties of biological samples. The talk is focused on the design, fabrication and validation of phantoms with adjustable phase, refractive index distribution, absorption, scattering etc., and their applications.
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Quantitative phase imaging with two in-line holograms
Optics LettersQuantitative phase imaging (QPI) has emerged as a practical technique for acquiring structural information from phase objects. Digital holography can realize phase detection, but it is limited by a spatial bandwidth product or affected by the overlap of conjugate images.
Xiu Wen +3 more
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All‐Optical Phase Recovery: Diffractive Computing for Quantitative Phase Imaging
Advanced Optical Materials, 2022Deniz Mengu, Aydoḡan Ozcan
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