Results 51 to 60 of about 36,100 (258)
Jurnal Teknologi dan Industri Pangan, 2006 Bixin belongs to the carotenoid group, from the Bixa orellana tree. It has conjugated double bonds which plays a role as an antiphotooxidant. The objectives of this study were to know the quenching mechanisms and kinetics of bixin in photosensitized ...
Ardiyanto Pranata1) +3 more
doaj
Molecules, 2004 The preparation and characterization by LCMS of a library of 55 fluorescence- quenched peptides is described. The peptides bear a terminal anthranilamide fluorophore and a penultimate 2,4-dinitrophenyl-L-lysine quencher, and will be used to probe the ...
Peter J. Duggan +2 more
doaj +1 more source
Quencher as Leaving Group: Efficient Detection of DNA-Joining Reactions
, 2016 We describe a new fluorescence reporting strategy in which dabsyl, a well-known quencher, activates a hydroxyl group in a probe to convert it to a leaving group.
Shinsuke Sando (1685644) +1 more
core +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
FEBS Open Bio, EarlyView.Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley +1 more source
Efficient Nucleic Acid Detection by Templated Reductive Quencher Release
, 2016 Efficient Nucleic Acid Detection by Templated Reductive Quencher ...
Eric T. Kool (1310808) +1 more
core +1 more source
FEBS Open Bio, EarlyView.The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer +7 more
wiley +1 more source
Biosensor Based on Aptamer Cross-Linked Hydrogel with Fluorescence “Turn-on” Properties for One-Step Detection of Zearalenone [PDF]
Shipin KexueObjective: To develop an aptamer-crosslinked fluorescent hydrogel biosensor for the detection of zearalenone (ZEN) in foods. Methods: Primer strand A and strand B modified with Black Hole Quencher 1 (BHQ1) were copolymerized under specific conditions to ...
LI Kejing, XIE Gang, DOU Jinxin, ZHANG Hongpeng, LUO Fei, GUO Yuyao, HAN Yitao
doaj +1 more source
Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins
FEBS Open Bio, EarlyView.Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley +1 more source
Проблемы особо опасных инфекций, 2017 Objective of the study was to optimize technological scheme for the production of preparation for gene diagnostics of plague with hybridization-fluorescent registration (HFR) of results, which is associated with the assessment and selection of synthesis ...
A. V. Stepanov +2 more
doaj +1 more source