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Fluorescence quenching of tryptophan by trifluoroacetamide
Biochimica et Biophysica Acta (BBA) - General Subjects, 1984Trifluoroacetamide was found to be a good quencher of tryptophan fluorescence, and the quenching was shown to proceed via both a dynamic and a static process. The respective quenching constants were determined by the measurement of the decrease of the fluorescence lifetime in the presence of the quencher.
Midoux, Patrick +3 more
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Fluorescence quenching of acridines by strandin
Archives of Biochemistry and Biophysics, 1961Abstract The interaction of four acridine compounds with strandin has been studied, with special attention being directed to acriflavine. In all cases fluorescence quenching by strandin occurs, the nature of the quenching curve depending on the dye involved.
A F, HARRIS, A, SAIFER, S K, WEINTRAUB
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Fluorescence Quenching Reactions
1991The quenching of the fluorescence of biomacromolecules by solute quenchers has become a widely used and powerful technique (Lehrer, 1976; Lehrer and Leavis, 1989; Lakowicz, 1983; Eftink and Ghiron, 1981; Eftink, 1991). Such quenching reactions have been used primarily to obtain topographical information about proteins, nucleic acids, and membrane ...
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Concentration Quenching of Nd^3+ Fluorescence
Applied Optics, 1968Concentration quenching of the 1.06-micro Nd(3+) fluorescence in glass is investigated to help determine the nature of the Nd(3+)-Nd(3+) interaction that causes quenching. The relative fluorescent efficiency vs Nd(3+) concentration is measured for thin, polished plates of glass in an apparatus designed to minimize instrumental distortion of the ...
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Quenched Fluorescent Substrate- Based Peptidase Assays
2005The use of specific quenched fluorescent substrates (QFS) provides a rapid and sensitive method to measure peptidase activity, and is readily adaptable to high-throughput screening of potential peptidase inhibitors. In this chapter, we discuss general considerations for the development of QFS assays, and describe in detail an assay protocol for the ...
Rebecca A, Lew +5 more
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Apparent Concentration Quenching of Morphine Fluorescence
Science, 1963Fundamental fluorescence equations were examined. Deviations from the theoretical were observed by varying the light path length to determine effects on "apparent" fluorescence. It was found that the decrease in emission of morphine solutions with the increase in concentration was caused by absorption effects that prevented excitation of the whole ...
R, Brandt, M J, Olsen, N D, Cheronis
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Chlorophyll fluorescence quenching by xanthophylls
Physical Chemistry Chemical Physics, 2003The interactions of the xanthophylls zeaxanthin, antheraxanthin and violaxanthin with chlorophyll, which are relevant for the chlorophyll fluorescence quenching in the non-photochemical quenching (NPQ) process in green plants, are investigated by means of quantum chemical methods.
Andreas Dreuw +2 more
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Charge Transfer and Fluorescence Quenching
The Journal of Chemical Physics, 1960A charge-transfer-interaction mechanism has been proposed for the quenching of fluorescence of a number of polynuclear aromatic hydrocarbons by aromatic solvents, especially methylated benzenes. The quenching efficiency has been found to be in the order benzene<toluene<xylene<mesitylene, which is also the order in which ionization ...
Dilip Kuman Majumdar, Sadhan Basu
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