Results 41 to 50 of about 5,353,584 (244)

CRISPR GUARD protects off-target sites from Cas9 nuclease activity using short guide RNAs

Nature Communications, 2020
Off-target editing remains a concern for therapeutic applications of CRISPR-Cas9. Here the authors present CRISPR GUARD, which uses very short non-cleaving gRNAs to prevent editing at off-target sites.
Matthew A. Coelho, Etienne De Braekeleer, Mike Firth, Michal Bista, Sebastian Lukasiak, Maria Emanuela Cuomo, Benjamin J. M. Taylor   +6 more
doaj   +1 more source

In vitro biological assessment of the stability of cigarette smoke aqueous aerosol extracts

BMC Research Notes, 2020
Objectives Cigarette smoke aqueous aerosol extracts (AqE) have been used for assessing tobacco products, particularly with in vitro models such as oxidative stress and inflammation.
Mark Taylor, Simone Santopietro, Andrew Baxter, Nicole East, Damien Breheny, David Thorne, Marianna Gaça   +6 more
doaj   +1 more source

Deep Sequencing of FLT3‐ITD Enables Response Evaluation and Post‐Treatment Monitoring in Childhood AML: An Exploratory Study

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Background An internal tandem duplication in the gene encoding Fms‐like tyrosine kinase 3 (FLT3‐ITD) is associated with high relapse risk and poor prognosis in acute myeloid leukemia (AML) and plays a crucial role in treatment decisions. Measurable residual disease (MRD) analysis of FLT3‐ITD during and after treatment has shown prognostic ...
Sofie Johansson Alm, Bea Tornberg, Erik Delsing Malmberg, Henrik Hasle, Kristian Løvvik Juul‐Dam, Lars Palmqvist, Borhan R. Saeed, Giti Shah Barkhordar, Anastasia Soboli, Anna Staffas, Jonas Abrahamsson, Linda Fogelstrand   +11 more
wiley   +1 more source

Time Toxicity in Wilms Tumor: Quantifying the Burden of Healthcare Interaction in the First Year After Diagnosis

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Background Wilms tumor (WT) treatment imposes a significant time burden on patients and their families. Time toxicity is a patient‐centered metric that quantifies the burden of healthcare interaction. We sought to define time toxicity in the first year after diagnosis of WT and hypothesized that it would increase as tumor stage and treatment ...
Caleb Q. Ashbrook, Veerain Gupta, Nicholas J. Clark, Vijay Rings, Lauren E. Corona, Douglass B. Clayton, Daniel J. Benedetti   +6 more
wiley   +1 more source

The ATR inhibitor ceralasertib potentiates cancer checkpoint immunotherapy by regulating the tumor microenvironment

Nature Communications
The Ataxia telangiectasia and Rad3-related (ATR) inhibitor ceralasertib in combination with the PD-L1 antibody durvalumab demonstrated encouraging clinical benefit in melanoma and lung cancer patients who progressed on immunotherapy.
Elizabeth L. Hardaker, Emilio Sanseviero, Ankur Karmokar, Devon Taylor, Marta Milo, Chrysis Michaloglou, Adina Hughes, Mimi Mai, Matthew King, Anisha Solanki, Lukasz Magiera, Ricardo Miragaia, Gozde Kar, Nathan Standifer, Michael Surace, Shaan Gill, Alison Peter, Sara Talbot, Sehmus Tohumeken, Henderson Fryer, Ali Mostafa, Kathy Mulgrew, Carolyn Lam, Scott Hoffmann, Daniel Sutton, Larissa Carnevalli, Fernando J. Calero-Nieto, Gemma N. Jones, Andrew J. Pierce, Zena Wilson, David Campbell, Lynet Nyoni, Carla P. Martins, Tamara Baker, Gilberto Serrano de Almeida, Zainab Ramlaoui, Abdel Bidar, Benjamin Phillips, Joseph Boland, Sonia Iyer, J. Carl Barrett, Arsene-Bienvenu Loembé, Serge Y. Fuchs, Umamaheswar Duvvuri, Pei-Jen Lou, Melonie A. Nance, Carlos Alberto Gomez Roca, Elaine Cadogan, Susan E. Critichlow, Steven Fawell, Mark Cobbold, Emma Dean, Viia Valge-Archer, Alan Lau, Dmitry I. Gabrilovich, Simon T. Barry   +55 more
doaj   +1 more source

Emapalumab for Immune Effector Cell‐Associated Hemophagocytic Lymphohistiocytosis‐Like Syndrome Following CD19‐Directed CAR‐T in Two Patients With B‐ALL: Clinical and Biomarker Correlates

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Immune effector cell‐associated hemophagocytic lymphohistiocytosis‐like syndrome (IEC‐HS) is a life‐threatening hyperinflammatory toxicity distinct from cytokine release syndrome (CRS) and neurotoxicity following chimeric antigen receptor T‐cell (CAR‐T) therapy. In a single‐institution retrospective cohort of pediatric and young adult patients
Thomas J. Galletta, Jeremy D. Rubinstein, Christopher E. Dandoy, Ruby Khoury, Angela Faulhaber, Christine L. Phillips, Christa Krupski   +6 more
wiley   +1 more source

Mechanics in the service of electricity generation

Mechanics & Industry
Electricity generation relies on a set of complex facilities where mechanical engineering plays an essential role in ensuring the performance, reliability, safety, and sustainability of systems.
Leclercq Sylvain, Abbas Mickaël, Alves Fernandes Vinicius, Benhamadouche Sofiane, Chapuliot Stéphane, Hendili Sofiane, Lorentz Eric, Michel Ponnelle Sylvie, Moussou Pierre, Robin Vincent, Violeau Damien, Fichet Vincent, Goreaud Nicolas, Pacull Julien, Moussallam Nadim, Martinez Philippe, Perrin Gilles   +16 more
doaj   +1 more source

The Role of Hematopoietic Cell Transplantation in Ataxia‐Telangiectasia

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Background Ataxia‐telangiectasia (A‐T) is a DNA repair disorder characterized by neurodegeneration, immunodeficiency, and cancer predisposition. Hematopoietic cell transplantation (HCT) is an established therapy in related disorders such as Fanconi anemia (FA) and Nijmegen breakage syndrome (NBS), but its role in A‐T is unclear.
Laila Alkhouli, Seth Rotz, Masatoshi Takagi, Richa Sharma   +3 more
wiley   +1 more source

MIMIC-BP: A curated dataset for blood pressure estimation

Scientific Data
Blood pressure (BP) is one of the most prominent indicators of potential cardiovascular disorders. Traditionally, BP measurement relies on inflatable cuffs, which is inconvenient and limit the acquisition of such important health-related information in ...
Ivandro Sanches, Victor V. Gomes, Carlos Caetano, Lizeth S. B. Cabrera, Vinicius H. Cene, Thomas Beltrame, Wonkyu Lee, Sanghyun Baek, Otávio A. B. Penatti   +8 more
doaj   +1 more source

Application of replication-defective West Nile virus vector to non-flavivirus vaccine targets

Human Vaccines & Immunotherapeutics, 2017
The RepliVax vaccine platform(RV) is based on flavivirus genomes that are rationally attenuated by deletion. The self-limiting infection provided by RV has been demonstrated to be safe, highly immunogenic and efficacious for several vaccine candidates ...
Maryann Giel-Moloney, Michael Vaine, Linong Zhang, Mark Parrington, Beata Gajewska, Thorsten U. Vogel, Svetlana O. Pougatcheva, Xiaochu Duan, Timothy Farrell, Irina Ustyugova, Sanjay Phogat, Harry Kleanthous, Konstantin V. Pugachev   +12 more
doaj   +1 more source