Results 61 to 70 of about 18,190,094 (297)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Comprehensive analysis on investigating water-saving potentials of irrigated cotton in semi-arid area in China

Agricultural Water Management
Deficit irrigation is a common strategy to reduce water use and improve the sustainability of cotton production. However, the effects of water deficits on crop productivity and quality are subject to genotype by management by environmental interactions ...
Xiaoyu Zhi, Barbara George-Jaeggli, Yingchun Han, Qiaomin Chen, Shijie Zhang, Lu Feng, Yaping Lei, Xiaofei Li, Guoping Wang, Zhanbiao Wang, Shiwu Xiong, Minghua Xin, Yahui Jiao, Yunzhen Ma, Yabing Li, Beifang Yang   +15 more
doaj   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Effect of terahertz irradiation on DNA damage repair in living cells

FEBS Open Bio, EarlyView.
We investigated the effect of terahertz (THz) wave irradiation on DNA double‐strand break (DSB) repair in living cells. We found that THz irradiation enhanced DSB repair at specific frequencies, whereas heat treatment inhibited it, indicating that this effect is nonthermal and frequency‐specific.
Yuya Ueno, Shota Yamazaki, Hiromichi Hoshina, Masahiko Harata   +3 more
wiley   +1 more source

Combination of Nitrogen and Potassium Improve Radiation Use Efficiency, Growth, Yield, and Agronomic Efficiency in Maize Cultivation

Plantropica: Journal of Agricultural Science
Proper management of nitrogen (N) and potassium (K) is needed to increase maize productivity in the area having low soil fertility due to their crucial role for plant growth and production.
Nur Azizah, Ellis Nihayati, Eko Widaryanto, Yogi Sugito   +3 more
doaj   +1 more source

Sorghum dwarfing genes can affect radiation capture and radiation use efficiency [PDF]

Field Crops Research, 2013
Presence of the dw3 sorghum dwarfing gene had negative effects on grain yield in some genetic backgrounds and environments. In a previous study we showed that this was due to a significant reduction in shoot biomass (mainly via reduced stem mass), which in turn negatively affected grain size. The current study examines whether shoot biomass was reduced
George-Jaeggli, B., Jordan, D. R., van Oosterom, E. J., Broad, I. J., Hammer, G. L.   +4 more
openaire   +4 more sources

Optimizing photoexcitation conditions for time‐resolved X‐ray solution scattering experiments

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering (TR‐XSS) is a powerful technique to visualize how proteins change their structure in real time after light activation. Selecting the right laser photoexcitation conditions—fluence, excitation geometry, and sample refresh rate—is critical to maximize the experimental signal while avoiding unwanted side effects ...
Matteo Levantino
wiley   +1 more source

Type I interferons modulate autophagy to shape gemcitabine response in pancreatic cancer cells

FEBS Open Bio, EarlyView.
Type I interferons differentially modulate autophagy and the response of pancreatic cancer cells to gemcitabine. IFNα2b stimulates autophagic flux and protects cells from gemcitabine‐induced cell death, contributing to chemoresistance. In contrast, IFNβ1a inhibits autophagosome formation and enhances gemcitabine‐induced cell death, resulting in ...
Lucy E. Bonilla, Martín M. Ledesma, Santiago A. Behr, Matías A. Pibuel, María L. Palanek, Daniel H. Grasso, Maria N. Garcia, Juan Garona, Silvina L. Lompardía, Elida Álvarez, Daniela L. Papademetrio   +10 more
wiley   +1 more source

Seasonal variation of radiation interception and radiation use efficiency in sugar beet (Beta vulgaris L.)

پژوهشهای زراعی ایران, 2009
A high correlation existes between crop growth and the rate of radiation intercepted. The efficiency of radiation interception and absorption is dependent on leaf area index, light extinction coefficient and radiation use efficiency.
sohail parsa, alireza kochaki, mahdi nasiri mahalati, alireza ghaemi   +3 more
doaj   +1 more source