Results 221 to 230 of about 73,981 (239)
Some of the next articles are maybe not open access.
Journal of Endocrinology, 1978
The evaluation of a radioimmunoassay of oxytocin is described. The method involved careful collection and transportation of blood at 4 °C, acidification of the plasma, extraction with Fuller's earth and radioimmunoassay using antisera raised in rabbits immunized against oxytocin conjugated to bovine serum albumin and 125I-labelled oxytocin.
M. Y. Dawood, K. S. Raghavan, C. Pociask
openaire +3 more sources
The evaluation of a radioimmunoassay of oxytocin is described. The method involved careful collection and transportation of blood at 4 °C, acidification of the plasma, extraction with Fuller's earth and radioimmunoassay using antisera raised in rabbits immunized against oxytocin conjugated to bovine serum albumin and 125I-labelled oxytocin.
M. Y. Dawood, K. S. Raghavan, C. Pociask
openaire +3 more sources
A radioimmunoassay of androstenedione
Steroids, 1973Abstract A dextran-coated charcoal radioimmunoassay for androstenedione (4-androsten-3, 17-dione) is reported which uses an anti-testosterone antiserum raised in sheep, against a testosterone-17-hemisuccinate-Bovine Serum Albumin conjugate. It is more sensitive and rapid than previously published double dilution, gas chromatographic and competitive ...
Stephen A. Tillson +3 more
openaire +3 more sources
A radioimmunoassay for progesterone
Steroids, 1972Abstract The need for a simple and fast quantitative method for progesterone with routine laboratory and clinical application motivated the development of a radioimmunoassay. Progesterone antibodies were produced in rabbits by immunization with a bovine serum albumin conjugate of 11α-hydroxyprogesterone hemisuccinate.
R. J. Saldarini +3 more
openaire +3 more sources
Biochimica et Biophysica Acta (BBA) - Protein Structure, 1977
Abstract A sensitive and specific radioimmunoassay has been developed for rat ligandin, capable of measuring this protein in the manogram range. The assay employs [125I] ligandin prepared by the chloramine-T method and rabbit antiserum to rat ligandin, with a double antibody method of separating antibody-bound from free ligandin.
Stuart J. Saunders +3 more
openaire +3 more sources
Abstract A sensitive and specific radioimmunoassay has been developed for rat ligandin, capable of measuring this protein in the manogram range. The assay employs [125I] ligandin prepared by the chloramine-T method and rabbit antiserum to rat ligandin, with a double antibody method of separating antibody-bound from free ligandin.
Stuart J. Saunders +3 more
openaire +3 more sources
Scandinavian Journal of Gastroenterology, 1991
Histamine is formed by decarboxylation of the amino acid histidine and is found both in plants and in animals, including man. In man it has important biologic functions. To assess the physiologic role of histamine, however, it is necessary to have a reliable and convenient method to determine its concentration in biologic fluids and tissue.
B. Schulze Søgnen +3 more
openaire +3 more sources
Histamine is formed by decarboxylation of the amino acid histidine and is found both in plants and in animals, including man. In man it has important biologic functions. To assess the physiologic role of histamine, however, it is necessary to have a reliable and convenient method to determine its concentration in biologic fluids and tissue.
B. Schulze Søgnen +3 more
openaire +3 more sources
Radioimmunoassay for rhodopsin
Experimental Eye Research, 1980Abstract A radioimmunoassay for rhodopsin was devised using [125I]rhodopsin and antibody to purified bovine rhodopsin in a double antibody procedure that can quantify the concentration of this visual pigment when present in picomolar amounts. The method demonstrated a high degree of sensitivity, reproducibility, and specificity, and is applicable to ...
James J. Plantner +2 more
openaire +3 more sources
A radioimmunoassay for metoclopramide
Journal of Immunological Methods, 1987A radioimmunoassay for the anti-emetic drug, metoclopramide, in the pmol range was developed. The immunogen was prepared by photolytic coupling of metoclopramide to bovine serum albumin. A crosslinking reagent, N-hydroxy-succinimidyl-4-azidobenzoate, was first reacted with serum albumin through nucleophilic substitution. Ultraviolet irradiation (lambda
B. J. Van Der Walt +3 more
openaire +2 more sources
Radioimmunoassay for sulprostone
Prostaglandins, Leukotrienes and Medicine, 1982The development of a radioimmunoassay for measuring subnanogram amounts of the prostaglandin uterine stimulant, sulprostone (N-methanesulfonyl 16-phenoxy-omega-tetranor PGE2 carboxamide), is described. The 9-carboxymethoxime derivative of sulprostone was coupled to keyhole limpet hemocyanin to prepare the immunogen, and to tyramine to yield a precursor
Thomas J. Carty +2 more
openaire +3 more sources
Radioimmunoassay of Proteoglycans
Journal of Immunoassay, 1984Human articular cartilage proteoglycan monomers (PG) were purified and used for developing a radioimmunoassay. Its analytical sensitivity is 0.6 ng/tube and its clinical sensitivity is 20 microliter/tube for serum and 0.12 microliter/tube for synovial fluid.
P. Gysen, Paul Franchimont
openaire +3 more sources
Seminars in Nuclear Medicine, 1975
The powerful nuclear medicine tool of radioimmunoassay was very rapidly developed because of the favorable properties of insulin in such a procedure. The current method of insulin immunoassay in our laboratory is described, with emphasis on the details of the separation of bound and free using talc tablets; emphasis is also given to the correction for ...
openaire +3 more sources
The powerful nuclear medicine tool of radioimmunoassay was very rapidly developed because of the favorable properties of insulin in such a procedure. The current method of insulin immunoassay in our laboratory is described, with emphasis on the details of the separation of bound and free using talc tablets; emphasis is also given to the correction for ...
openaire +3 more sources