Characterization of propionate CoA-transferase from Ralstonia eutropha H16
Applied Microbiology and Biotechnology, 2013In this study, a propionate CoA-transferase (H16_A2718; EC 2.8.3.1) from Ralstonia eutropha H16 (Pct(Re)) was characterized in detail. Glu342 was identified as catalytically active amino acid residue via site-directed mutagenesis. Activity of Pct(Re) was irreversibly lost after the treatment with NaBH₄ in the presence of acetyl-CoA as it is shown for ...
Elena, Volodina +3 more
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Repression of Phenol Catabolism by Organic Acids in Ralstonia eutropha
Applied and Environmental Microbiology, 1998ABSTRACT During batch growth of Ralstonia eutropha (previously named Alcaligenes eutrophus ) on phenol in the presence of acetate, acetate was found to be the preferred substrate; this organic acid was rapidly metabolized, and the specific rate of phenol consumption was considerably
F, Ampe, D, Léonard, N D, Lindley
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Formaldehyde degradation byRalstonia eutrophain an immobilized cell bioreactor
Journal of Environmental Science and Health, Part A, 2013The formaldehyde (FA) degradation ability of the loofa-immobilized Ralstonia eutropha cells in a packed bed reactor was modeled using a statistically based design of the experiment (DOE) considering application of response surface methodology (RSM).
Alireza, Habibi, Farzaneh, Vahabzadeh
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Genetics and control of CO 2 assimilation in the chemoautotroph Ralstonia eutropha
Archives of Microbiology, 2002The nutritional versatility of facultative autotrophs requires efficient overall control of their metabolism. Most of these organisms are Proteobacteria that assimilate CO(2) via the highly energy-demanding Calvin-Benson-Bassham reductive pentose-phosphate cycle.
Botho, Bowien, Bernhard, Kusian
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State observation for fedbatch control of phenol degradation by Ralstonia eutropha
1999 European Control Conference (ECC), 1999This paper concerns the design of a control system for fedbatch phenol degradation by Ralstonia eutropha. The control objective was to maintain the specific growth rate at a constant high value, or equivalently to regulate the phenol concentration at a constant low residual value. This objective was achieved thanks to a PI controller.
Isabelle Queinnec +2 more
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Production of two phase polyhydroxyalkanoic acid granules in Ralstonia eutropha
International Journal of Biological Macromolecules, 1999To synthesize layered granules consisting of selected phases of polyhydroxybutyrate (PHB) homopolymer and PH(B-co-V) copolymer, Ralstonia eutropha was grown on fructose and limited quantities (1 g/l) of valeric acid. Exhaustion of the valerate resulted in a carbon source shift and a shift in the composition of polyhydroxyalkanoate (PHA) being ...
A S, Kelley, F, Srienc
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Solvent production by engineered Ralstonia eutropha: channeling carbon to biofuel
Applied Microbiology and Biotechnology, 2018Microbial production of solvents like acetone and butanol was a couple of the first industrial fermentation processes to gain global importance. These solvents are important feedstocks for the chemical and biofuel industry. Ralstonia eutropha is a facultatively chemolithoautotrophic bacterium able to grow with organic substrates or H2 and CO2 under ...
Jayashree, Chakravarty +1 more
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The hydrogen-sensing apparatus in Ralstonia eutropha.
Journal of molecular microbiology and biotechnology, 2002Molecular hydrogen is widely used by microorganisms as a source of energy. One of the best studied aerobic hydrogen oxidizers, the beta-proteobacterium Ralstonia eutropha (formerly Alcaligenes eutrophus), harbors two distinct [NiFe]-hydrogenases which catalyze the heterolytic cleavage of H2 into 2H+ and 2e-.
Oliver, Lenz +4 more
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Metabolic engineering of Ralstonia eutropha for the production of polyhydroxyalkanoates from sucrose
Biotechnology and Bioengineering, 2014ABSTRACTA sucrose utilization pathway was established in Ralstonia eutropha NCIMB11599 and R. eutropha 437‐540 by introducing the Mannheimia succiniciproducens MBEL55E sacC gene that encodes β‐fructofuranosidase. These engineered strains were examined for the production of poly(3‐hydroxybutyrate) [P(3HB)] and poly(3‐hydroxybutyrate‐co‐lactate) [P(3HB ...
Park, SJ Park, Si Jae +11 more
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Production of polyhydroxybutyrate by Ralstonia eutropha from protein hydrolysates
Applied Microbiology and Biotechnology, 1998Polyhydroxybutyrate (PHB) was produced by Ralstonia eutropha DSM 11348 (formerly Alicaligenes eutrophus) in media containing 20–30 g l−1 casein peptone or casamino acids as sole sources of nitrogen. In fermentations using media based on casein peptone, permanent growth up to a cell dry mass of 65 g l−1 was observed.
E. J. Bormann +4 more
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