RanGTP regulates the augmin complex
2022SUMMARY Spindles are composed of microtubules that must nucleate at the right place and time during mitosis. Spindle microtubule nucleation is regulated by the GTPase Ran, which, through importin-αβ, releases a gradient of spindle assembly factors (SAFs) centered at chromosomes.
Jodi Kraus +3 more
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Nuclear RanGTP is not required for targeting small nucleolar RNAs to the nucleolus [PDF]
Journal of Cell Science, 2003 The small GTPase Ran is the central regulator of macromolecular transport between the cytoplasm and the nucleus. Recent work has suggested that RanGTP also plays an important role in regulating some intra-nuclear processes. In this study, we have investigated whether RanGTP is required for the intra-nuclear transport of RNAs.
Ian G Macara +2 more
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The Role of RanGTP Gradient in Vertebrate Oocyte Maturation
2011The maturation of vertebrate oocyte into haploid gamete, the egg, consists of two specialized asymmetric cell divisions with no intervening S-phase. Ran GTPase has an essential role in relaying the active role of chromosomes in their own segregation by the meiotic process. In addition to its conserved role as a key regulator of macromolecular transport
Petr, Kaláb, Petr, Solc, Jan, Motlík
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Hyperosmotic Stress Signaling to the Nucleus Disrupts the Ran Gradient and the Production of RanGTP [PDF]
Molecular Biology of the Cell, 2007 The RanGTP gradient depends on nucleocytoplasmic shuttling of Ran and its nucleotide exchange in the nucleus. Here we show that hyperosmotic stress signaling induced by sorbitol disrupts the Ran protein gradient and reduces the production of RanGTP. Ran gradient disruption is rapid and is followed by early (10–20 min) and late (30–60 min) phases of ...
Joshua B Kelley, Bryce M Paschal
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Supraphysiological nuclear export signals bind CRM1 independently of RanGTP and arrest at Nup358 [PDF]
EMBO Journal, 2004 Leucine-rich nuclear export signals (NESs) mediate rapid nuclear export of proteins via interaction with CRM1. This interaction is stimulated by RanGTP but remains of a relatively low affinity. In order to identify strong signals, we screened a 15-mer random peptide library for CRM1 binding, both in the presence and absence of RanGTP.
Jero Calafat, Maarten Fornerod
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A Mitotic Lamin B Matrix Induced by RanGTP Required for Spindle Assembly
Science, 2006Mitotic spindle morphogenesis is a series of highly coordinated movements that lead to chromosome segregation and cytokinesis. We report that the intermediate filament protein lamin B, a component of the interphase nuclear lamina, functions in spindle assembly.
Ming-Ying, Tsai +6 more
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Human RanBP3, a group of nuclear RanGTP binding proteins.
FEBS letters, 1998A group of novel human Ran-binding proteins, RanBP3, was identified using the yeast two-hybrid system via Ran-mediated interaction with the nucleotide exchange factor RCC1. Several open reading frames, representing putative alternatively spliced products, were established by cDNA cloning.
L, Mueller +3 more
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Characterization of the Effects of RanGTP on the Microtubule Cytoskeleton
2003Sofia B, Lizarraga +2 more
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Mechanism of RanGTP priming H2A-H2B release from Kap114 in an atypical RanGTP•Kap114•H2A-H2B complex
Proceedings of the National Academy of Sciences of the United States of America, 2023Jenny Jiou +2 more
exaly
Molecular basis of RanGTP-activated release of Histones H2A-H2B from Importin-9
Structure, 2023Joy M Shaffer +2 more
exaly