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Optimizing preparation of renal biopsy specimens for TEM: a time-efficient approach to rapid diagnosis of kidney diseases [PDF]

open access: yesRenal Failure
Electron microscopic examination of renal biopsies is crucial for the diagnosis of kidney disease. In this study, we present an optimized protocol that significantly reduces processing time to 19.5 h, compared to 120 h with conventional methods, while ...
Gen Wang   +5 more
doaj   +2 more sources

A Review of Biofilm Formation of Staphylococcus aureus and Its Regulation Mechanism

open access: yesAntibiotics, 2022
Bacteria can form biofilms in natural and clinical environments on both biotic and abiotic surfaces. The bacterial aggregates embedded in biofilms are formed by their own produced extracellular matrix. Staphylococcus aureus (S. aureus) is one of the most
Qi Peng   +4 more
doaj   +1 more source

The molybdate-binding protein ModA is required for Proteus mirabilis-induced UTI

open access: yesFrontiers in Microbiology, 2023
BackgroundProteus mirabilis is one of the pathogens commonly causing urinary tract infections (UTIs). The molybdate-binding protein ModA encoded by modA binds molybdate with high affinity and transports it.
Yi Huang   +12 more
doaj   +1 more source

Schistosome egg-derived extracellular vesicles deliver Sja-miR-71a inhibits host macrophage and neutrophil extracellular traps via targeting Sema4D

open access: yesCell Communication and Signaling, 2023
Background Macrophages and neutrophils are rapidly recruited around Schistosome eggs to form granulomas. Extracellular traps (ETs) of macrophages and neutrophils are part of the pathogen clearance armamentarium of leukocytes. Schistosome eggs possess the
Yao Liao   +8 more
doaj   +1 more source

The gene regulatory role of non-coding RNAs in non-obstructive azoospermia

open access: yesFrontiers in Endocrinology, 2022
Non-coding RNAs are classified as small non-coding RNAs, long non-coding RNAs and circular RNAs, which are involved in a variety of biological processes, including cell differentiation, proliferation, apoptosis and pathological conditions of various ...
Guanqing Zhou   +15 more
doaj   +1 more source

Highly sensitive quantitative phase microscopy and deep learning aided with whole genome sequencing for rapid detection of infection and antimicrobial resistance

open access: yesFrontiers in Microbiology, 2023
Current state-of-the-art infection and antimicrobial resistance (AMR) diagnostics are based on culture-based methods with a detection time of 48–96 h. Therefore, it is essential to develop novel methods that can do real-time diagnoses.
Azeem Ahmad   +8 more
doaj   +1 more source

Loop-Mediated Isothermal Amplification Coupled With Nanoparticle-Based Lateral Biosensor for Rapid, Sensitive, and Specific Detection of Bordetella pertussis

open access: yesFrontiers in Bioengineering and Biotechnology, 2022
Bordetella pertussis is the most frequent causative agent for pertussis, which is a highly contagious disease. Here, we developed a method based on loop-mediated isothermal amplification (LAMP) and nanoparticle-based lateral flow biosensor (LFB) for the ...
Chunrong Sun   +7 more
doaj   +1 more source

RETRACTED: Chemotherapy-induced broadly reactive autoantibodies in the treatment of malignancies

open access: yesВестник медицинского института «Реавиз»: Реабилитация, врач и здоровье, 2023
RETRACTED ARTICLEThe aim of the study was to evaluate the relationship between chemotherapy and autoimmune reactions in patients with metastatic colorectal cancer. Cancer and autoimmunity are known to be interrelated, but until now it has been unclear to
E. V. Shanina   +5 more
doaj   +1 more source

Rapid diagnosis of sepsis [PDF]

open access: yesVirulence, 2013
Fast and appropriate therapy is the cornerstone in the therapy of sepsis. However, the discrimination of sepsis from non-infectious causes of inflammation may be difficult. Biomarkers have been suggested to aid physicians in this decision. There is currently no biochemical technique available which alone allows a rapid and reliable discrimination ...
Bloos, Frank, Reinhart, Konrad
openaire   +2 more sources

Rapid Onsite Visual Detection of Orf Virus Using a Recombinase-Aided Amplification Assay

open access: yesLife, 2023
Orf is an important zoonotic disease caused by the Orf virus (ORFV) which can cause contagious pustular dermatitis in goats and sheep. Orf is widespread in most sheep-raising countries in the world, causing huge economic losses.
Huan Cui   +8 more
doaj   +1 more source

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