Results 261 to 270 of about 837,711 (307)

Rapid DNA Sequence Analysi

CRC Critical Reviews in Biochemistry, 1979
(1979). Rapid DNA Sequence Analysi. CRC Critical Reviews in Biochemistry: Vol. 6, No. 1, pp. 1-33.
Gillian M. Air, Heinz Schaller
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Rapid filter method for the microfluorometric analysis of DNA

Analytical Biochemistry, 1975
Abstract A rapid filter method for the microfluorometric analysis of DNA is reported in this communication. Cells collected on cellulose filters are subject to an assay sequence developed from a fluorometric method initially described by J. M. Kissane and E. Robins ((1958) J. Biol. Chem. 233 , 184–188) for DNA quantitation. The assay is one of high
R A, Cattolico, S P, Gibbs
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A rapid computer analysis for multicomponent DNA reassociation kinetics

Analytical Biochemistry, 1977
Abstract An iterative program, Gaushaus, has been adapted to analyze complex DNA reassociation kinetics. With valid experimental data, rapid convergence to a solution is always achieved by this program even when the starting parameters are grossly in error. The output of the program also includes useful statistical information.
D I, Kells, N A, Straus
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Rapid analysis of DNA strand breaks in soft tissues

Environmental Mutagenesis, 1985
AbstractA technique was developed to measure rapidly DNA strand breaks in soft tissues. This method measured the rate of alkaline unwinding of DNA, which was proportional to strand breakage. Alkaline unwinding of DNA was done by treating tissue homogenates with NaOH. Single‐stranded DNA was removed by extraction with aqueous phenol.
S R, Morris, H G, Shertzer
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Rapid Preparation of DNA for RFLP Analysis and DNA Fingerprinting

1990
The standard procedure for preparation of eucaryotic DNA includes several time consuming steps such as digestion of proteins with proteinase K and subsequent extractions of aminoacids and protein fragments with phenol (Maniatis 1982). The method described here is based on protein disintegration with SDS-urea (Meinke 1974) instead of proteinase K.
G. Holmlund, P. Hyltén, K. Friberg, B. Lindblom   +3 more
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Thermal Performance Analysis of a Silicon Microreactor for Rapid DNA Analysis

Thermal and Thermomechanical Proceedings 10th Intersociety Conference on Phenomena in Electronics Systems, 2006. ITHERM 2006., 2006
This paper describes the use of thermal modelling tools in the design and characterisation of a multi-function silicon microreactor polymerase chain reaction (PCR) thermocycler system for rapid DNA diagnostic assays. MEMS technologies have been successfully applied to this application and the miniaturization of devices offers several advantages ...
A.K. Gnanappa, null Cathy Ke, O. Slattery, M. Sheehan   +3 more
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Method for rapid isolation and analysis of algal virus DNA

Journal of Virological Methods, 1988
A quick method has been developed for isolating viral DNA from small cultures of eukaryotic algae infected with PBCV-1 or similar viruses. The DNA preparations are virtually free of contaminating host DNA and are suitable substrates for restriction enzymes. 5 ml cultures yield 8-25 micrograms of viral DNA.
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Minisatellite DNA Profiles: Rapid Sample Identification in Linkage Analysis

Human Heredity, 1990
Locus-specific minisatellite probes detect multiple alleles with heterozygosities of greater than 90% when hybridised to HinfI and AluI restriction digests of human DNA. We have hybridised 4 of these probes to a panel of DNAs digested with 6 of the restriction enzymes which commonly reveal di-allelic polymorphisms in the human genome.
H, Telenius, J, Clark, E, Marcus, N, Royle, A J, Jeffreys, B A, Ponder, C G, Mathew   +6 more
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A procedure for rapid isolation and analysis of cauliflower mosaic virus DNA

Virology, 1980
A simple and rapid procedure has been developed for the small-scale isolation of crude preparations of cauliflower mosaic virus DNA. The preparations are relatively free of contaminating cellular DNA and are sufficiently pure to be digested with restriction enzymes. The method enables plants infected with single-lesion isolates of mutagenized viral DNA
R C, Gardner, R J, Shepherd
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Rapid DNA purification for restriction fragment length polymorphism analysis

Gene Analysis Techniques, 1988
This paper describes a method for isolation of DNA from blood samples involving a rapid chemical disintegration of proteins with 8 M urea and with a minimum of exposure to phenol. The DNA is further desalted and purified on Sephadex G-25 prepacked disposable columns.
B, Lindblom, G, Holmlund
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