Results 21 to 30 of about 837,711 (307)

Complementary DNA Significantly Enhancing Signal Response and Sensitivity of a Molecular Beacon Probe to Aflatoxin B1

open access: yesBiosensors, 2023
This paper reported an improved molecular beacon method for the rapid detection of aflatoxin B1 (AFB1), a natural mycotoxin with severe carcinogenicity. With the assistance of a complementary DNA (cDNA) chain, the molecular beacon which consists of a DNA
Chao Wang   +3 more
doaj   +1 more source

Novel approach for deriving genome wide SNP analysis data from archived blood spots [PDF]

open access: yes, 2012
BACKGROUND: The ability to transport and store DNA at room temperature in low volumes has the advantage of optimising cost, time and storage space.
Walling, Grant A   +12 more
core   +1 more source

Fluorescence in situ hybridization of YAC clones after Alu-PCR amplification [PDF]

open access: yes, 1992
Alu-PCR protocols were optimized for the generation of human DNA probes from yeast strains containing yeast artificial chromosomes (YACs) with human inserts between 100 and 800 kb in size.
Cremer, Thomas   +2 more
core   +1 more source

Rapid Competitive PCR Using Melting Curve Analysis for DNA Quantification

open access: yesBioTechniques, 2001
A rapid competitive PCR method was developed to quantify DNA on the LightCycler®. It rests on the quantitative information contained in the melting curves obtained after amplification in the presence of SYBR® Green I.
Samiya Al-Robaiy   +2 more
doaj   +1 more source

Stories in ancient dna

open access: yesBioTechniques, 2017
Technology developments are leading to rapid advances in ancient DNA analysis. Nathan Blow talks to researchers peering into the past through ancient DNA.
Nathan Blow
doaj   +1 more source

Collecting, archiving and processing DNA from wildlife samples using FTA® databasing paper [PDF]

open access: yes, 2004
Background Methods involving the analysis of nucleic acids have become widespread in the fields of traditional biology and ecology, however the storage and transport of samples collected in the field to the laboratory in such a manner to allow ...
LA Burgoyne   +3 more
core   +1 more source

Rapid Profiling Cell Cycle by Flow Cytometry Using Concurrent Staining of DNA and Mitotic Markers

open access: yesBio-Protocol, 2017
The flow cytometric quantitation of DNA content by DNA-binding fluorochrome, propidium iodide (PI) is the most widely used method for cell cycle analysis. However, the commonly used methods are time-consuming and labor-intensive and are incompatible with
Yuqing Shen   +2 more
doaj   +1 more source

The effects of freezing on faecal microbiota as determined using MiSeq sequencing and culture-based investigations. [PDF]

open access: yesPLoS ONE, 2015
High-throughput sequencing has enabled detailed insights into complex microbial environments, including the human gut microbiota. The accuracy of the sequencing data however, is reliant upon appropriate storage of the samples prior to DNA extraction. The
Fiona Fouhy   +7 more
doaj   +1 more source

Rapid procedure for isolation of plasmid DNA and application to epidemiological analysis [PDF]

open access: yesJournal of Clinical Microbiology, 1984
A rapid and simple plasmid isolation procedure was developed for the epidemiological analysis of plasmid-mediated antimicrobial resistance. By this method, plasmid DNAs ranging in molecular weight between 2.0 and 122 X 10(6) could be detected. Various bacteria, such as strains of the family Enterobacteriaceae, Pseudomonas aeruginosa, Haemophilus ...
S, Takahashi, Y, Nagano
openaire   +2 more sources

Test and Evaluation of the Made-in-China Quick TargSeq Fully Integrated Automated On-site Rapid DNA Detection Instrument

open access: yesFayixue Zazhi
ObjectiveTo verify the stability and accuracy of the made-in-China Quick TargSeq Fully Integrated Automated On-site Rapid DNA Detection Instrument (hereinafter referred to as Quick TargSeq DNA detection instrument) and evaluate its forensic application ...
HAN Jun-ping   +9 more
doaj   +1 more source

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