Results 181 to 190 of about 4,554,614 (308)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Developing and evaluating a novel rapid test for recent HIV-1 infection: comparison with commercial LAg-EIA assays. [PDF]

J Clin Microbiol
Liu J, Liu P, Ye R, Yao S, Zhu Q, Jiang Y, Jin C.   +6 more
europepmc   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Quantum-enhanced nanodiamond rapid test advances early SARS-CoV-2 antigen detection in clinical diagnostics. [PDF]

Nat Commun
Thomas DeCruz A, Miller BS, Huang D, McRobbie M, Donaldson F, McCoy LE, O'Sullivan CK, Botha JC, Nastouli E, McKendry RA.   +9 more
europepmc   +2 more sources

Dual Treponemal and Non-Treponemal Rapid Test for Syphilis in People Living with HIV: A Diagnostic Accuracy Study. [PDF]

Sex Transm Dis
Fuertes-Bucheli JF, Silva-Peña SA, Hurtado-Rossi L, Ospina-Cortes C, Ramírez LG, Martinez-Valencia AJ, Hawley KL, Seña AC, Garcia-Luna JA.   +8 more
europepmc   +1 more source

Evaluation of the Panbio™ COVID-19 IgG rapid test device performance. [PDF]

Heliyon, 2023
Moy JN, Amin AM, Chalmers-Watson C, Chowdhury R, Forssten C, Fu J, Ghosh S, Harris JD, Kordowich S, Li Y, Lin W, Mackay-Thomas S, Mickiewicz M, Patel N, Resino S, Sevenoaks T, Tugetman MA, Valencia J, Vijesurier R, White N, Woods CW, Kennedy PT, Ryan P.   +22 more
europepmc   +1 more source

Quantitative proteomic analysis reveals different characteristics of bladder cancer cells after exposure to bisphenol A

FEBS Open Bio, EarlyView.
Bisphenol A (BPA), a common chemical in plastics, exerts dual effects on bladder cancer cells: low doses promote growth and migration, while high doses suppress growth and migration. Multi‐omics and bioinformatics reveal BPA acts via MAPK and inflammatory pathways.
Shaomin Niu, Lanlan Li, Wenjuan Jia, Hanzhong Feng, Xiaoquan Yu, Youli Zhao, Fengzhi Yuan, Hui Ding, Junqiang Tian, Yong‐Xing He, Zhiping Wang   +10 more
wiley   +1 more source

Diagnostic accuracy of the sickle SCAN^® rapid test for neonatal screening of sickle cell disease in Lubumbashi, Democratic Republic of the Congo. [PDF]

Ann Hematol
Katamea T, Mukuku O, Mutombo AK, Luboya ON, Wembonyama SO.   +4 more
europepmc   +1 more source

Iron homeostasis disruption and lipid peroxidation in skeletal muscle during short‐term immobilization

FEBS Open Bio, EarlyView.
14‐day casting‐induced immobilization reduced gastrocnemius muscle mass and increased non‐heme iron and ferritin heavy chain levels. Despite iron accumulation, transferrin receptor 1 and iron regulatory protein 2 were paradoxically upregulated. Lipid peroxidation was elevated without compensatory antioxidant responses.
Haruka Yokogawa, Kazuhiko Higashida, Naoya Nakai   +2 more
wiley   +1 more source

Development of a Fluorescent Rapid Test Sensing System for Influenza Virus. [PDF]

Micromachines (Basel)
Weng WC, Wu YL, Lin ZJ, Pan WF, Lin YC.
europepmc   +1 more source