Results 161 to 170 of about 1,195,962 (266)

Characterization of Carcinogenesis Resistance in Anatolian Blind Mole-rat Mesenchymal Stem Cells and Assessment of Transcriptomic Signatures. [PDF]

Stem Cells
Unver N, Tavukcuoglu E, Budak B, Sezginer O, Ozturk SC, Ozturk SE, Taskiran EZ, Kankilic T, Arga KY, Esendagli G.   +9 more
europepmc   +1 more source

Engineering IL‐4 resistant proinflammatory human myeloid cells for cancer immunotherapy

Molecular Oncology, EarlyView.
We developed a scalable workflow to generate proinflammatory human myeloid cells. CRISPR/Cas9‐edited CD34+ hematopoietic stem and progenitor cells were expanded and differentiated with M‐CSF. Deletion of STAT6 or STAT6/NFKB1 enhanced macrophage proinflammatory gene expression and cytokine secretion in the presence of IL‐4 while maintaining antibody ...
Theresa Barberi, Alan D. Friedman
wiley   +1 more source

Comprehensive metabolomic profiling of Angiostrongylus cantonensis across its life cycle reveals developmental and host-associated metabolic signatures. [PDF]

Infect Dis Poverty
Hu Y, Yu B, Zhan R, Zhang T, Zhang Y, Zhou Y, Wei H, Lv Z.   +7 more
europepmc   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Morphological and molecular characterization of an undescribed vestibuliferid ciliate from the captive-bred naked mole-rat, Heterocephalus glaber (Rodentia: Bathyergidae). [PDF]

Int J Parasitol Parasites Wildl
Takase M, Tokiwa T.
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Limited detection of Gulf War illness-like behavioral phenotypes in Charles River male Sprague-Dawley rats across multiple chemical and stress exposure paradigms. [PDF]

PLoS One
Lashley R, Foster JB, Wang X, Muhleman AJ, Weaver S, Lin E, Lai L, Lin CG.   +7 more
europepmc   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Statement of Retraction: Dexmedetomidine protects H9C2 rat cardiomyocytes against hypoxia/reoxygenation injury by regulating the long non-coding RNA colon cancer-associated transcript 1/microRNA-8063/Wnt/β-catenin axis. [PDF]

Bioengineered
europepmc   +1 more source