Results 201 to 210 of about 3,207,063 (305)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Host-anellovirus interactions in an island ecosystem: non-human primates and rodents in Madagascar harbour diverse, rich anellovirus populations. [PDF]

Microb Genom
Paietta EN, Johnston RA, Randrianarisoa SF, DeSisto CMM, Kraberger S, Martin D, Razanamahenina TT, Ramboninarimalala A, Velontsara JB, Raherinirina TG, Rasendrinirina V, Raveloson L, Luo Y, Finley NL, Baitchman E, Yoder AD, Varsani A.   +16 more
europepmc   +1 more source

Exon 7 splicing of ERα predicts poor prognosis and increases phenotypic heterogeneity in luminal a subtype breast cancer

FEBS Open Bio, EarlyView.
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui, Taobo Hu, Thomson Christian Husein, Wai Hei Shek, Bingrong Chen, Depeng Wang, Shu Wang, Weichuan Yu, Xiaodan Fan, Mengping Long, Toyotaka Ishibashi   +10 more
wiley   +1 more source

A bioabsorbable adhesive wrap for sutureless arterial repair: Initial development and preclinical testing in a rat model. [PDF]

J Trauma Acute Care Surg
Nithianandam P, Hesek A, Steinmetz M, Mills J, Liu R, Corao-Uribe D, Shaffer T, Blauvelt DG, Wilmington, Delaware.   +9 more
europepmc   +1 more source

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

Enable preclinical, high-precision image-guided radiotherapy research with a stand-alone small animal IGRT system. [PDF]

Med Phys
Deng W, Han D, Byrne KE, Bhandary B, Jiang K, Poirier YP, Mossahebi S, Xu J, Shukla H, Sawant A.   +9 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Robust CD8⁺ T cell responses induced by an mRNA-LNP vaccine encoding rat HER2 extracellular domain confer prophylactic tumor protection. [PDF]

Front Immunol
Li K, Li X, Zhang C, Dai S, Li L, Wang J, Zhang H, Zhao L.   +7 more
europepmc   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Real-Time Feedback Strategically Regulates Optoelectronics for Customized Optogenetic Spinal Cord Regeneration. [PDF]

Exploration (Beijing)
Xia K, Hu R, Zhou X, Chen J, You X, Wang J, Li H, Tao Y, Yu C, Xu H, Zhang Y, Shi K, Li Y, Wang C, Li F, Liang C, Chen Y, Chen Q.   +17 more
europepmc   +1 more source