Results 241 to 250 of about 6,418,597 (300)

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

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Rated Tags: Adding Rating Capability to Collaborative Tagging

2013 International Conference on Cloud and Green Computing, 2013
Collaborative tagging is a popular way to organize content. But sometimes it is also used as a way to express opinions, which is normally done through rating- or text review systems. This paper will demonstrate that there is a gap between rating- and text review systems, that limits the ability of users to find the desired item.
Daniel Kailer, Peter Mandl 0001, Alexander Schill   +2 more
openaire   +1 more source

Evaluating the interrater agreement of process capability ratings

Computer Standards & Interfaces, 1997
The reliability of process assessments has received some study in the recent past, much of it being conducted within the context of the SPICE (Software Process Improvement and Capability dEtermination) trials. In this paper, we build upon this work by evaluating the reliability of ratings on each of the practices that make up the SPICE capability ...
Pierfrancesco Fusaro, Khaled El Emam, Bob Smith   +2 more
openaire   +1 more source

Rate Capability Testing of Graphite Electrode Material

ECS Transactions, 2021
The paper deals with the investigation of natural graphite electrode materials for lithium-ion batteries. These negative electrode materials operate on the intercalation principle where graphite plays a host role for lithium ions. There is a solid electrolyte interphase (SEI) layer which origins from electrode-electrolyte interphase. The SEI layer is a
Jiri Libich, Josef Maca, Marie Sedlarikova, Antonín Šimek, Pavel Cudek, Andrey Chekannikov, Guenter Fafilek   +6 more
openaire   +1 more source

Rate capability of spinel LiCr0.1Ni0.4Mn1.5O4

Journal of Alloys and Compounds, 2010
Abstract The spinel compound LiCr 0.1 Ni 0.4 Mn 1.5 O 4 is synthesized by a sol–gel method. In this synthesizing process, Li(CH 3 COO)·2H 2 O, Ni(CH 3 COO) 2 ·4H 2 O, Mn(CH 3 COO) 2 ·4H 2 O and Cr(NO 3 ) 3 ·9H 2 O are used as reactants, and malic acid as chelating agent. The reaction takes place at 900 °C for 8 h. The electrochemical performances of
G.Q. Liu, L. Wen, G.Y. Liu, Y.W. Tian
openaire   +1 more source