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Ribosomal RNA and rDNA sequence analyses
Gene, 1992The potential of ribosomal (r) RNA and the encoding genes (rDNA) to elucidate natural relationships has been dramatically extended by improved sequencing approaches and the application of polymerase chain reaction. Sequence information on 16S and 23S rRNA/DNA from 69 strains of 53 Streptomyces species allows determination of regions that can be used as
Stackebrandt, Erko +2 more
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Amplification of rDNA and type I sequences in Drosophila males deficient in rDNA
Cell, 1983rDNA magnification is a heritable change in rDNA content that occurs in D. melanogaster males when chromosomes deficient in rDNA are placed together for several generations. We have examined the restriction endonuclease cleavage pattern of the rDNA from an X chromosome undergoing magnification, and find no evidence for the selective amplification of ...
D V, de Cicco, D M, Glover
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rDNA magnification in D. melanogaster: State of rDNA copies following the first step
Molecular and General Genetics MGG, 1979D. melanogaster males of XYbb/O genetic constitution undergoing rDNA magnification were mated singly to XXbb+/O females, yielding XYbb/O male progeny, and to XNO- w sn bb+ females, yielding XYbb/XNO- females. The male and female offspring were scored for the bb+ phenotype.
Graziani F, LA MANTIA, GIROLAMA
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SSU rDNA phylogeny of cladoniiform lichens
American Journal of Botany, 1998To examine phylogenetic relationships among the “cladoniiform” lichenized fungi, i.e., the families Cladoniaceae, Baeomycetaceae, Icmadophilaceae, Stereocaulaceae, and Siphulaceae, and to provide evidence for the anticipated independent origins of podetia and pseudopodetia, we conducted phylogenetic analyses of SSU (small subunit) rDNA sequences from ...
S K, Stenroos, P T, Depriest
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16S rDNA methods in soil microbiology
Current Opinion in Biotechnology, 1999With the introduction of molecular methods, the past decade has seen renewed interest in soil microbiology. New and exciting molecular technologies and the promise of finally opening the microbial black box in soil drive much of this interest. Although these pioneering studies have added much to our knowledge of microbial diversity in soils, it is ...
, O'Donnell, , Gorres
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rDNA Transcription during Xenopus laevis Oogenesis
Biochemical and Biophysical Research Communications, 2002It is generally believed that, during Xenopus laevis oogenesis, polymerase I transcription is high in the early vitellogenic oocytes (stages III and IV) and very low in later stages. We used a combination of RNA labeling, nuclease S1 protection assays, Northern blot, and half-life measurement of preribosomal RNA to reinvestigate the pattern of ...
Roger, B. +3 more
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Regulation of rDNA stability by sumoylation
DNA Repair, 2009Repair of DNA lesions by homologous recombination relies on the copying of genetic information from an intact homologous sequence. However, many eukaryotic genomes contain repetitive sequences such as the ribosomal gene locus (rDNA), which poses a risk for illegitimate recombination.
Eckert-Boulet, Nadine, Lisby, Michael
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rDNA Amplification: Application of 16S rDNA-Based Methods for Bacterial Identification
2000Continuing improvements in PCR and automated sequencing technologies, resulting in rapid acquisition of sequence data, have allowed these methods to be applied to the task of bacterial identification. The sequence of the 16S rDNA/RNA of bacterial strains has been demonstrated to be useful for phylogenetic analysis of different taxa.
Frederick A. Rainey, Erko Stackebrandt
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การจำแนกสปีชีส์ของเชื้อมัยโคแบคทีเรียมโดยหาลำดับเบสของชิ้นส่วน 16S rDNA ที่เพิ่มจำนวน
ทำการศึกษาเพื่อแยกสปีชีส์ของเชื้อมัยโคแบคทีเรีย โดยการหาลำดับเบสของชิ้นส่วน 16S rDNA ที่เพิ่มจำนวนด้วยเทคนิค polymerase chain reaction เปรียบเทียบกับวิธีที่ใช้อยู่ประจำ (conventional method) โดยอาศัยลักษณะของโคโลนี การสร้างสี อัตราการเจริญเติบโตปฏิกิริยาชีวเคมี และ Accuprobe ในการศึกษาใช้เชื้อมัยโคแบคทีเรียมาตรฐาน 16 สปีชีส์ และเชื้อมัยโคแบคทีเรียท ...openaire +1 more source

