Results 61 to 70 of about 2,217,168 (245)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

Mechanical properties of sandwich composites with 3d-printed auxetic and non-auxetic lattice cores under low velocity impact

Materials & Design, 2018
This work presents a comparative study on the reliability of auxetic (re-entrant honeycomb) and non-auxetic (diamond lattice and conventional honeycomb) lattice composites.
Shaoyu Hou, Tiantian Li, Zian Jia, Lifeng Wang   +3 more
doaj   +1 more source

Structure‐forward targeting of claudins with synthetic binders

FEBS Letters, EarlyView.
Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley   +1 more source

DYNAMIC CRUSHING PERFORMANCE ANALYSIS FOR AUXETIC HONEYCOMB STRUCTURE

Jixie qiangdu, 2016
The in-plane dynamic crushing behavior of a new honeycomb structure-the multi-re-entrant honeycomb and reentrant honeycomb topology possessing negative Poisson’s ratio was analyzed here.
HOU XiuHui, YIN GuanSheng
doaj  

Enhanced mechanical performance of re-entrant structures using carbon fiber/polyamide composites by dual-material 3D printing

Journal of Materials Research and Technology
Multi-component 3D printing has become a reliable and feasible strategy with complex shape and colorful appearance. However, the overall mechanical performance is greatly dependent on the material components and printing pattern design.
Hao Zhang, Lihan Wang, Hongyu Zhang, Pan He, Lin Sang   +4 more
doaj   +1 more source

Discerning protein pools by selective staining with self‐labeling tags

FEBS Letters, EarlyView.
Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley   +1 more source

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source

APPROXIMATE ANALYSIS OF RE-ENTRANT LINES WITH BERNOULLI RELIABILITY MODELS [PDF]

, 2007
Re-entrant lines are widely used in many manufacturing systems, such as semiconductor, electronics, etc. However, the performance analysis of re-entrant lines is largely unexplored due to its complexity. In this thesis, we present iterative procedures to
Wang, Chong
core   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source