Results 141 to 150 of about 1,620,370 (218)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Dual-Sabatier Optima: How Reaction Mechanism Determines Activity Volcano Map of Dual-Atom Catalysts for Oxygen Reduction Reaction. [PDF]

Angew Chem Int Ed Engl
Liu J, Li H, Xu H, Cheng D.
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Crystallographic snapshots of Trypanosoma cruzi aspartate transcarbamoylase including catalytic intermediates suggest an ordered Bi-Bi reaction mechanism. [PDF]

Sci Rep
Matoba K, Nara T, Aoki T, Honma T, Tanaka A, Inoue M, Matsuoka S, Harada S, Kita K, Inaoka DK, Balogun EO, Shiba T.   +11 more
europepmc   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Efficient Catalytic Ozonation of Dimethyl Carbonate on M‑Mn (M = Cr, Cu, Co)/ZSM‑5 Zeolites: Further Analysis of Reaction Mechanism. [PDF]

ACS Omega
Sun Y, Zhang Y, He Y, Weng W, Zhu Y, Liu P, Wang Z.   +6 more
europepmc   +1 more source

One size does not fit all: An in vitro evaluation of the effects of bezafibrate and medroxyprogesterone acetate on human SH‐SY5Y and U‐87 MG cancer cells

FEBS Open Bio, EarlyView.
Drugs previously repurposed to target blood cancers reduced neuroblastoma and glioblastoma cell growth and viability. However, their levels of anticancer activity were different and their clinical application may be problematic due to side effects at effective doses.
Abhishek Kharawatkar, Francesco Michelangeli, Farhat Latif Khanim, Christopher Martin Bunce, William Eustace Basil Johnson   +4 more
wiley   +1 more source

Isolation, Antioxidant Activity, and Reaction Mechanism of Chamazulene from <i>Artemisia selengensis</i> Turcz. ex Besser Essential Oil. [PDF]

ACS Omega
Wang Z, Zhang S, He Y, Yamazaki S, Ma S, Li Z, Shang M, Miao X, Ma Y.   +8 more
europepmc   +1 more source

Lipopolysaccharide uptake is augmented in lipopolysaccharide‐tolerant mouse macrophage‐like cells via increased CD14 expression

FEBS Open Bio, EarlyView.
In normal (nontolerant) cells, CD14 is crucial for both LPS uptake and LPS signaling. In LPS‐tolerant cells, in which LPS‐induced TNF‐α and IFN‐β production is suppressed, there is a dramatic increase in surface CD14 expression. The overexpressed CD14 in LPS‐tolerant cells is responsible for the enhanced LPS uptake without inducing pro‐inflammatory ...
Saeka Nishihara, Takayuki Manabe, Mika Jouta, Kiyoshi Kawasaki   +3 more
wiley   +1 more source

Quasi-1D Chain-Based Zirconium Trisulfide as a Low-Potential High-Rate Anode: Structural and Reaction Mechanism Insights. [PDF]

ACS Appl Mater Interfaces
Wei S, Liu M, Yu R, Jiang H, Zhou H, Li H, Li M, Chauhan P, Wu B, Matsuo T, Mosina K, Dekanovsky L, Oliveira FM, Luxa J, Jankovsky O, Su J, Sofer Z.   +16 more
europepmc   +1 more source