Results 21 to 30 of about 35,689 (162)

Evaluation of a commercial ELISA kit for the detection of anti-dengue IgM in serum of patients from a hospital in the north of Peru

Revista Electrónica Dr. Zoilo E. Marinello Vidaurreta, 2021
Background: capture-Igm (immunoglobulin) ELISA (enzyme-linked immunosorbent assay) is the main immunoenzymatic assay used in the diagnosis of dengue, for this purpose there are several commercial tests with different output.Objective: to evaluate the ...
Christian Junnior Campos-Monteza, Heber Silva-Díaz, Danny Omar Suclupe-Campos, Franklin Rómulo Aguilar–Gamboa   +3 more
doaj  

GroEL Chaperonin-Based Assay for Early Diagnosis of Scrub Typhus

Diagnostics, 2022
A point-of-care diagnostic for early and rapid diagnosis of scrub typhus caused by Orientia tsutsugamushi is required for prompt and proper treatment of patients presenting with undifferentiated febrile illnesses.
Nitaya Indrawattana, Pisinee Aiumurai, Nawannaporn Sae-lim, Watee Seesuay, Onrapak Reamtong, Manas Chongsa-nguan, Wanpen Chaicumpa, Nitat Sookrung   +7 more
doaj   +1 more source

Evaluation of miniaturized Illumina DNA preparation protocols for SARS-CoV-2 whole genome sequencing.

PLoS ONE, 2023
The global pandemic caused by SARS-CoV-2 has increased the demand for scalable sequencing and diagnostic methods, especially for genomic surveillance.
Sureshnee Pillay, James Emmanuel San, Derek Tshiabuila, Yeshnee Naidoo, Yusasha Pillay, Akhil Maharaj, Ugochukwu J Anyaneji, Eduan Wilkinson, Houriiyah Tegally, Richard J Lessells, Cheryl Baxter, Tulio de Oliveira, Jennifer Giandhari   +12 more
doaj   +2 more sources

Diagnostic performance evaluation of urine HIV-1 antibody rapid test kits in a real-life routine care setting in China

BMJ Open
Objectives To evaluate the diagnostic performance of urine HIV antibody rapid test kits in screening diverse populations and to analyse subjects’ willingness regarding reagent types, purchase channels, acceptable prices, and self-testing.Designs ...
Huanhuan Chen, Qiuying Zhu, Guanghua Lan, Yuhua Ruan, Mei Lin, Shujia Liang, Jianjun Li, Huaxiang Lu, Guangjie Tan, Xianwu Pang, Xianmin Ge, Yunxian Huang, Zhenqiang Chen, Shizhen Zhang, Wenlong Cai   +14 more
doaj   +1 more source

Synthesis and herbicidal evaluation of N-cinnamoyl-N-substituted hydroxylamine and its derivatives

Revista Brasileira de Engenharia de Biossistemas
The group of cinnamon and hydroxylamine produces significant results in the field of agriculture. So, the combination of both groups in the compound performs effective results in numerous fields of the medicinal and chemical industry.
Ajay Kumar, Sushila Arya, Sonakshi Chandra, Shreya Kotnala, Archana Goswami   +4 more
doaj   +1 more source

DEVELOPMENT OF HEPATITIS E 3 GENOTYPE RECOMBINANT PROTEIN CAPSID OF: CLONING, EXPRESSION, PURIFICATION, EVALUATION OF THE ANTIGENIC PROPERTIES

Журнал микробиологии, эпидемиологии и иммунобиологии, 2019
Aim. The development of the hepatitis E virus (HEV) genotype 3 recombinant capsid protein. Materials and methods. E.coli strains, plasmid vectors, serological and clinical samples, ELISA reagent kits, molecular biological, bioinformatic, biotechnological,
G. I. Alatortseva, A. V. Sidorov, L. N. Nesterenko, L. N. Luhverchik, V. V. Dotsenko, I. I. Amiantova, M. V. Zhukina, V. Yu. Kabargina, A. V. Milovanova, D. S. Vorobev, Yu. I. Ammur, M. I. Mikhailov, K. K. Kyuregyan, E. Yu. Malinnikova, S. V. Zhavoronok, V. M. Blinov, V. V. Zverev   +16 more
doaj   +1 more source

Phenotypic microbial response to antimicrobial exposure conditions with a molecular analysis quantification of species-specific 16S rRNA content

MethodsX, 2021
The emergence and rapid spread of resistant bacteria has become a serious public health concern worldwide. Delayed antimicrobial therapy significantly increases mortality in high-risk infections with a particularly strong association with septic shock ...
Jade Chen, Michael Tomasek, Eliseo Nuñez, Vincent Gau   +3 more
doaj   +1 more source

Advanced Laboratory Methods for Detecting Yellow Fever Pathogen

Проблемы особо опасных инфекций, 2021
Yellow fever is an acute infectious disease of viral nature, the causative agent of which is vector-borne –is transmitted through the bites of infected mosquitoes. Massive epidemics caused by the yellow fever virus are observed in the countries of Africa,
E. I. Krivosheina, M. Yu. Kartashov, E. V. Naidenova   +2 more
doaj   +1 more source

DESIGN OF HEPATITIS E VIRUS GENOTYPE 1 RECOMBINANT ORF3 PROTEIN BY CODON OPTIMIZATION METHOD

Журнал микробиологии, эпидемиологии и иммунобиологии, 2017
Aim. The development of the hepatitis E virus (HEV) genotype 1 full-size ORF3 recombinant polypeptide. Materials and methods. Escherichia coli strains, plasmid vectors, serological and clinical samples, ELISA reagent kits, molecular biological ...
G. I. Alatortseva, A. V. Sidorov, L. N. Nesterenko, L. N. Luhverchik, M. V. Zhukina, I. I. Amiantova, A. V. Milovanova, D. S. Vorobev, Yu. I. Ammur, M. I. Mikhailov, K. K. Kyuregyan, V. S. Kichatova, I. A. Potemkin, O. V. Isaeva, E. Yu. Malinnikova, A. A. Karlsen, V. M. Blinov, Z. Sh. Nurmatov, A. Z. Nurmatov, O. T. Kasymov, S. V. Zhavoronok, V. V. Zverev   +21 more
doaj   +1 more source

Screening of specific diagnostic peptides of swine hepatitis E virus

Virology Journal, 2009
Background Swine hepatitis E virus (swHEV) is a zoonotic disease that is considered a major problem in pig production and presents a threat to human health.
Zhu Hong, Li Jianyue, Li Zhen, Yu Ruisong, Liu Qiwen, Zhao Kai, Wu Xiao, Tan Furong, Wang Jinbin, Tang Xueming   +9 more
doaj   +1 more source