Results 191 to 200 of about 20,355,262 (262)

Development of a multiplex quantitative real-time PCR (qPCR) assay for simultaneous detection of Schistosoma haematobium and high-risk human papillomaviruses. [PDF]

PLoS Negl Trop Dis
Donkoh ET, Williams I, Akologo RN, Asiedu AA, Ofori-Atta RJ, Boadu IW, Ryabinina O, Forson AO, Boampong K, Jain N, Dassah ET, Gyasi SF, Arroyo Mühr LS, Akanwariwaik WG.   +13 more
europepmc   +1 more source

Spatially Controlled Embedded Bioprinting of Prevascularized Spheroids‐Containing Biopatch Enables Functional Skin Regeneration

Advanced Healthcare Materials, EarlyView.
A spatially controlled embedded bioprinting strategy enables precise engineering of prevascularized multicellular spheroids within skin‐derived dECM bioinks. Optimized spheroid size, composition, and spacing promote vascular maturation, paracrine signaling, and tissue integration, resulting in enhanced angiogenesis, blood perfusion, and functional skin
Minjun Ahn, Gyu Tae Park, Dongjun Lee, Sik Yoon, Sae‐Ock Oh, Jae Ho Kim, Byoung Soo Kim   +6 more
wiley   +1 more source

Development and Validation of Real-Time PCR for Detecting Anaplasma bovis-Like Agent in Dermacentor spp. Ticks. [PDF]

Emerg Infect Dis
Smith RC, Barrantes Murillo DF, Carr A, Duncan KT, Starkey LA.   +4 more
europepmc   +1 more source

Self‐Healing Hydrogel‐Enabled Modular Assembly of Bilayered Skin Construct for Hair Follicle Regeneration

Advanced Healthcare Materials, EarlyView.
Self‐healing assembly of two cell‐laden hydrogel layers creates a biomimetic skin construct that restores both dermal and epidermal functions, resulting in accelerated wound healing and enhanced hair follicle regeneration in a full‐thickness skin wound model. ABSTRACT Alopecia remains a pervasive clinical challenge, largely owing to the limited ability
JaeWook Park, Su Yong Kim, MinSu Jeong, Beom Joon Kim, Shin Hyuk Kang, Won‐Gun Koh   +5 more
wiley   +1 more source

Consensus framework for developing a target product profile of real-time PCR in Chagas disease therapeutic monitoring. [PDF]

PLoS Negl Trop Dis
Schijman AG, Forsyth C, Abras A, Almeida IC, Alonso-Padilla J, Alonso-Vega C, Ballart-Ferrer C, Bisio M, Britto C, Dumonteil E, Flores-Chavez M, Gallego M, Herrera-Bernal CP, Lopez-Albizu C, Moreira OC, Muñoz-Calderon A, Pinto L, Ramirez JC, Scandale I, Shikanai-Yasuda MA, Sosa-Estani S, Pinazo MJ.   +21 more
europepmc   +1 more source

Rational Control of Antibody Orientation for Enhancing Sensitivity and Specificity in Lateral Flow Immunoassays With Clinical Validation

Advanced Healthcare Materials, EarlyView.
Systematic investigation of antibody orientation strategies in lateral flow immunoassays reveals that a para‐substituted (aminomethyl)phenylboronic acid mediated probe (P‐6) combined with a biotin‐streptavidin test‐line modification achieves a synergistic 14‐ to 19‐fold sensitivity gain over conventional conjugation. Furthermore, multiplexed detection,
Pin‐Hao Wang, Shunmuga Nathan Shunmuga Nainar, Bing‐Han Zhuang, Shun‐Mao Yang, Hong‐Cheu Lin, Yang‐Hsiang Chan   +5 more
wiley   +1 more source

Evaluation of a portable multiplex real-time pcr assay for the detection of monkeypox virus and non-variola orthopoxviruses. [PDF]

Virol J
Li S, Das N, Miller N, Caudle A, Wilkins K, Khan T, Pfotenhauer S, Saunders A, Hutson CL, Venkateswaran KS, Nelson WM, Venkateswaran N, Satheshkumar PS, Townsend MB.   +13 more
europepmc   +1 more source

Macrophage–Fibroblast Crosstalk Governs Tumor Mechanics, as Revealed by Spatiotemporal Force Mapping in 3D Spheroids

Advanced Healthcare Materials, EarlyView.
This study shows crosstalk among tumor cells, fibroblasts, and macrophages alters the physical forces inside 3D tumor spheroids. Incorporating deformable microparticles in 3D tumor spheroids, the researchers mapped mechanical stresses spatially and with respect to tumor growth (temporally).
Kunal P. Pednekar, Daan Vorselen, Jai Prakash   +2 more
wiley   +1 more source

Optimisation and analytical assessment of a TaqMan™ probe-based real-time PCR assay designed to diagnose infection with Schistosoma japonicum. [PDF]

Parasit Vectors
Archer J, Hingel S, Abarsosa C, Zabala K, Nailes JM, Webster BL.   +5 more
europepmc   +1 more source

Hybrid Biofabrication of a Hierarchically Organized Periosteum‐Inspired In Vitro Platform

Advanced Healthcare Materials, EarlyView.
A hybrid biofabrication strategy integrates melt electrowriting with extrusion‐based 3D bioprinting to create a hierarchically organized periosteum‐inspired in vitro platform. Spatially defined osteogenic, fibrous, and endothelial cell‐containing compartments support long‐term culture, osteogenic maturation, and CD31+ endothelial cell organization ...
Yakui Liu, Xinggui Tian, Anne Bernhardt, Anja Lode, Max von Witzleben, Ege Kirbas, Stefan Zwingenberger, Michael Gelinsky   +7 more
wiley   +1 more source