Results 1 to 10 of about 1,797,163 (336)

Real-Time PCR

BioTechniques, 2008
AbstractReal‐time PCR is a recent modification to the polymerase chain reaction that allows precise quantification of specific nucleic acids in a complex mixture by fluorescent detection of labeled PCR products. Detection can be accomplished using specific, as well as nonspecific fluorescent probes.
Lynne Lederman
doaj   +4 more sources

Simulation of between repeat variability in real time PCR reactions. [PDF]

PLoS ONE, 2012
While many decisions rely on real time quantitative PCR (qPCR) analysis few attempts have hitherto been made to quantify bounds of precision accounting for the various sources of variation involved in the measurement process.
Antoon Lievens, Stefan Van Aelst, Marc Van den Bulcke, Els Goetghebeur   +3 more
doaj   +6 more sources

Real-time PCR for mRNA quantitation

BioTechniques, 2005
Real-time PCR has become one of the most widely used methods of gene quantitation because it has a large dynamic range, boasts tremendous sensitivity, can be highly sequence-specific, has little to no post-amplification processing, and is amenable to ...
Marisa L. Wong, Juan F. Medrano
doaj   +3 more sources

Real-Time PCR Assay for Quantitative Mismatch Detection [PDF]

BioTechniques, 2003
We describe here a quantitative realtime PCR assay for the detection of single-base-pair differences that does not require fluorescently labeled gene-specific probes or complicated primer combinations.
L. Shively, L. Chang, J.M. LeBon, Q. Liu, A.D. Riggs, J. Singer-Sam   +5 more
doaj   +2 more sources

Evaluation of a sensitive real-time PCR assay for Group B Streptococcus detection in vaginal-rectal swab. [PDF]

Sci Rep
Wang X, Wang N, Wang J, Zhang S, Zhao K, Yuan J.   +5 more
europepmc   +3 more sources

A MIQE-Compliant Real-Time PCR Assay for Aspergillus Detection [PDF]

, 2012
PMCID: PMC3393739This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are ...
AH Groll, AS Pham, B De Pauw, C Costa, C Girmenia, C Halliday, C Mengoli, Carol A. Munro, DA Tsitsikas, David F. Bibby, DS Perlin, DW Denning, Gemma L. Johnson, HA Torres, J Faber, J Loeffler, J Loeffler, JB Reitsma, JE Galagan, JR Perfect, M Kami, M Sanguinetti, M Subira, M Zuker, MJ Espy, ML Herrera, PD Khot, PD Khot, PL White, PL White, PL White, R Bu, S Ascioglu, S Challier, SA Bustin, SA Bustin, SA Bustin, SA Bustin, SA Bustin, Samir G. Agrawal, SM Duval, Stephen A. Bustin, Stephenie Wong, T Nolan, TF Patterson, Y Zhao, Y Zhao   +46 more
core   +17 more sources

Detection of Cephalosporin and Fluoroquinolone Resistance Genes via Novel Multiplex qPCR in Fecal Salmonella Isolates From Northern Californian Dairy Cattle, 2002–2016

Frontiers in Microbiology, 2021
The objectives of this study were to evaluate the prevalence of extended spectrum β-lactamase (ESBL) genes, AmpC-type β-lactamase (ACBL) genes, and plasmid mediated quinolone resistance (PMQR) genes in Salmonella isolated at a Veterinary Medical Teaching
Carl Basbas, Barbara A. Byrne, Munashe Chigerwe, Edlin D. Escobar, Emir Hodzic, Alda F. A. Pires, Richard V. Pereira   +6 more
doaj   +1 more source

PRIMA-1 inhibits Y220C p53 amyloid aggregation and synergizes with cisplatin in hepatocellular carcinoma

Frontiers in Molecular Biosciences, 2023
Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related deaths worldwide. Although many therapeutic options are available, several factors, including the presence of p53 mutations, impact tumor development and therapeutic resistance ...
Mariana M. Paz, Giulia D. S. Ferretti, Giulia D. S. Ferretti, Mafalda M. C. Martins-Dinis, Beatriz I. S. Ferreira, Amanda Faier-Pereira, Thibaut Barnoud, Otacilio C. Moreira, Jerson L. Silva, Yraima Cordeiro, Luciana P. Rangel   +10 more
doaj   +1 more source

Modulation of miR-145-5p and miR-146b-5p levels is linked to reduced parasite load in H9C2 Trypanosoma cruzi infected cardiomyoblasts

Scientific Reports, 2022
In the heart tissue of acutely Trypanosoma cruzi-infected mice miR-145-5p and miR-146b-5p are, respectively, downregulated and upregulated. Here, we used the H9C2 rat cardiomyoblast cell line infected with the Colombian T. cruzi strain to investigate the
Priscila Silva Grijó Farani, Beatriz Iandra Silva Ferreira, Daniel Gibaldi, Joseli Lannes-Vieira, Otacilio Cruz Moreira   +4 more
doaj   +1 more source

Monitoring for freshwater mussel presence in rivers using environmental DNA

Environmental DNA, 2021
In 2013, the U.S. Environmental Protection Agency (USEPA) updated its national recommended water quality criteria for the protection of aquatic life from the toxic effects of ammonia in freshwaters.
Ellen P. Preece, Michael Bryan, Samantha M. Mapes, Cara Wademan, Robert Dorazio   +4 more
doaj   +1 more source