Results 11 to 20 of about 20,354,300 (293)
Real-time PCR for mRNA quantitation
BioTechniques, 2005 Real-time PCR has become one of the most widely used methods of gene quantitation because it has a large dynamic range, boasts tremendous sensitivity, can be highly sequence-specific, has little to no post-amplification processing, and is amenable to ...
Marisa L. Wong, Juan F. Medrano
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BioTechniques, 2008 AbstractReal‐time PCR is a recent modification to the polymerase chain reaction that allows precise quantification of specific nucleic acids in a complex mixture by fluorescent detection of labeled PCR products. Detection can be accomplished using specific, as well as nonspecific fluorescent probes.
Lynne Lederman
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Real-time PCR is based on the conventional principles of PCR and, with the simple shift of emphasis from the end-product to the whole course of the PCR process, has established itself as the most sensitive and specific quantitative PCR method.
Dorak MT
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Real-time PCR in virology [PDF]
Nucleic Acids Research, 2002 The use of the polymerase chain reaction (PCR) in molecular diagnostics has increased to the point where it is now accepted as the gold standard for detecting nucleic acids from a number of origins and it has become an essential tool in the research laboratory.
Mackay, I. M., Arden, K. E., Nitsche, A.
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Development of a real-time multiplex PCR assay for the detection of multiple Salmonella serotypes in chicken samples. [PDF]
, 2008 peer-reviewedBackground: A real-time multiplex PCR assay was developed for the detection of multiple Salmonella serotypes in chicken samples. Poultry-associated serotypes detected in the assay include Enteritidis, Gallinarum, Typhimurium, Kentucky and ...
Sheila McGuinness +15 more
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Quantification of Fusarium graminearum and Fusarium culmorum by real-time PCR system and zearalenone assessment in maize [PDF]
, 2012 Zearalenone (ZEA) is a mycotoxin produced by some species of Fusarium, especially by Fusarium graminearum and F. culmorum. ZEA induces hyperoestrogenic responses in mammals and can result in reproductive disorders in farm animals. In the present study, a
Atoui, Ali +3 more
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A Parylene Real Time PCR Microdevice [PDF]
, 2010 The polymerase chain reaction (PCR) is a powerful biochemical assay that is used in virtually all biochemical labs. By specifically amplifying a small sample of DNA, this technique is useful in the fields of paternity testing, forensics, and virus ...
Quach, Quoc Chan (Brandon)
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A real-time PCR assay to estimate Leishmania chagasi load in its natural sand fly vector Lutzomyia longipalpis. [PDF]
, 2008 Leishmania chagasi, transmitted mainly by Lutzomyia longipalpis sand flies, causes visceral leishmaniasis and atypical cutaneous leishmaniasis in Latin America.
Ranasinghe, Shalindra +9 more
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Democratizing the Real-Time PCR [PDF]
Clinical Chemistry, 2017 Featured Article: Wittwer CT, Herrmann MG, Moss AA, Rasmussen RP. Continuous fluorescence monitoring of rapid cycle DNA amplification. Biotechniques 1997;22:130–1, 134–8.3 After the development of the PCR in the second half of the1980s and demonstration of real-time analysis in 1992 (1), it was not clear how the technology would unfold.
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Advances in Physiology Education, 2005 In recent years, real-time polymerase chain reaction (PCR) has emerged as a robust and widely used methodology for biological investigation because it can detect and quantify very small amounts of specific nucleic acid sequences. As a research tool, a major application of this technology is the rapid and accurate assessment of changes in gene ...
Mark A, Valasek, Joyce J, Repa
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