The Professional Medical Journal, 2012 In recent years, real-time PCR has come forward as a robust and widely used molecular technique in clinical and biologicalsettings. Although it can detect very minute quantities of target nucleic acid, but quantification of specific nucleic acids is not an easy task.Accurate and precise quantification is hampered by a number of factors that may include
ABIDA RAZA, NAUREEN A KHATTAK
openaire +3 more sources
Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions [PDF]
, 2003 BACKGROUND: Real-time PCR is increasingly being adopted for RNA quantification and genetic analysis. At present the most popular real-time PCR assay is based on the hybridisation of a dual-labelled probe to the PCR product, and the development of a ...
Bell, S.M. +13 more
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Novel real-time PCR assays for the specific detection of human infective Cryptosporidium species [PDF]
, 2016 Cryptosporidium is a protozoan parasite causing gastrointestinal illness. Drinking waterborne outbreaks have been caused by C. hominis, C. parvum and C. cuniculus.
Bouzid, Maha +5 more
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Comparison of subgingival bacterial sampling with oral lavage for detection and quantification of periodontal pathogens by real-time polymerase chain reaction [PDF]
, 2007 Background: Saliva has been studied for the presence of subgingival pathogens in periodontitis patients. With the anaerobic culture technique, the discrepancy between salivary recovery and subgingival presence has been significant, which makes this ...
Boutaga, Khalil +3 more
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Serology versus Real Time PCR in the Diagnosis of Human Brucellosis
Journal of Pure and Applied Microbiology, 2017 With the unwelcome re-emergence of brucellosis in different regions of the world, an accurate and timely diagnosis of this zoonosis has become a daunting challenge.
Sara El-Hossain Aly Reda +3 more
doaj +1 more source
Seroprevalence and Molecular Detection of Bovine Anaplasmosis in Egypt [PDF]
, 2020 Bovine anaplasmosis is a tick-borne disease with zoonotic potential, caused by the obligate intracellular bacterium Anaplasma marginale. The disease is distributed worldwide in tropical and subtropical regions.
El-Adawy, Hosny +5 more
core +1 more source
PCR REAL TIME PER L’IDENTIFICAZIONE DI MYCOBACTERIUM TUBERCULOSIS MDR
Microbiologia Medica, 2003 -
F. Meacci +5 more
doaj +1 more source
Recent sequence variation in probe binding site affected detection of respiratory syncytial virus group B by real-time RT-PCR [PDF]
, 2017 Background Direct immuno-fluorescence test (IFAT) and multiplex real-time RT-PCR have been central to RSV diagnosis in Kilifi, Kenya. Recently, these two methods showed discrepancies with an increasing number of PCR undetectable RSV-B viruses ...
Agoti, Charles N. +8 more
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مجلة كلية الطب, 2014 Background:-M. pneumoniae is an important human pathogen that produces community-acquired respiratory tract infection. Diagnosis of M. pneumoniae infection is challenging and crucial for the timely initiation of the effective antibiotic therapy ...
Muntaha M. Hassan +3 more
doaj +1 more source
Implementation of polymerase chain reaction (PCR) and Real-Time PCR in quick identification of bovine herpesvirus 1 [PDF]
Veterinarski Glasnik, 2010 Examinations were performed on 65 samples of nasal smeas taken from calves and young cows with clinical symptoms of respiratory infection to determine the presence of the bovine herpes virus 1 using parallel implementation of molecular and standard ...
Milić Nenad +6 more
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