Results 21 to 30 of about 1,466,444 (250)

Advanced and conventional molecular techniques in the diagnosis of Mycoplasma pneumoniae in patients with respiratory tract infection

مجلة كلية الطب, 2014
Background:-M. pneumoniae is an important human pathogen that produces community-acquired respiratory tract infection. Diagnosis of M. pneumoniae infection is challenging and crucial for the timely initiation of the effective antibiotic therapy ...
Muntaha M. Hassan, Mushtak T.S. Al-Ouqaili, Nidhal A. Mohammed, Omar A. Mohammed   +3 more
doaj   +1 more source

Real-Time PCR

The Professional Medical Journal, 2012
Abstract Realtime PCR (or qPCR) is a widely used gold of standard for quantitative analysis of gene expression. It is also a very sensitive method for viral copy number detection, and genotyping. This Protocol outlines qPCR theory, data analysis and detailed procedures.
ABIDA RAZA, NAUREEN A KHATTAK
openaire   +3 more sources

Protein and mRNA expression of estradiol receptors during estrus in yaks (Bos grunniens)

Revista Brasileira de Zootecnia, 2015
The objective of this study was to investigate mRNA by real-time PCR and protein expression by immunofluorescence of the estradiol receptors (ER) in the pineal gland, hypothalamus, pituitary gland, and gonads of yaks (Bos grunniens).
Shengdong Huo, Qijian Yuan, Taojie Zhang, Junlin Liu, Ayimuguli Abudureyimu, Jutian Yang   +5 more
doaj   +1 more source

Implementation of polymerase chain reaction (PCR) and Real-Time PCR in quick identification of bovine herpesvirus 1 [PDF]

Veterinarski Glasnik, 2010
Examinations were performed on 65 samples of nasal smeas taken from calves and young cows with clinical symptoms of respiratory infection to determine the presence of the bovine herpes virus 1 using parallel implementation of molecular and standard ...
Milić Nenad, Nišavić Jakov, Ašanin Ružica, Knežević Aleksandra, Ašanin Jelena, Vidanović D., Šekler Milanko   +6 more
doaj   +1 more source

Rapid and Sensitive Qualitative Duoplex Real-Time PCR Method for Discriminatory and Confirmatory Diagnosis of HTLV-1 and HTLV-2 Infections: Brazilian Multicentric Study

Frontiers in Medicine, 2022
Human T cell lymphotropic virus (HTLV) is the caustive agent of two main conditions i. e., the HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) and the adult T-cell leukemia/lymphoma (ATLL).
Mauricio Cristiano Rocha-Junior, Mauricio Cristiano Rocha-Junior, Evandra Strazza Rodrigues, Evandra Strazza Rodrigues, Svetoslav Nanev Slavov, Svetoslav Nanev Slavov, Tatiane Assone, Maíra Pedreschi, Debora Glenda Lima de La Roque, Debora Glenda Lima de La Roque, Maisa Sousa, Viviana Olavarria, Bernardo Galvão-Castro, Benedito Antonio Lopes da Fonseca, Augusto César Penalva de Oliveira, Jerusa Smid, Oswaldo Massaiti Takayanagui, Jorge Casseb, Dimas Tadeu Covas, Dimas Tadeu Covas, Dimas Tadeu Covas, Simone Kashima, Simone Kashima, Simone Kashima   +23 more
doaj   +1 more source

Statistical analysis of real-time PCR data

BMC Bioinformatics, 2006
Background Even though real-time PCR has been broadly applied in biomedical sciences, data processing procedures for the analysis of quantitative real-time PCR are still lacking; specifically in the realm of appropriate statistical treatment.
Reed Ann, Yuan Joshua S, Chen Feng, Stewart C Neal   +3 more
doaj   +1 more source

Development of New Modified Simple Polymerase Chain Reaction and Real-time Polymerase Chain Reaction for the Identification of Iranian Brucella abortus Strains [PDF]

Archives of Razi Institute, 2019
Brucellosis is primarily a worldwide zoonotic disease caused by Brucella species. The genus Brucella contains highly infectious species that are classified as biological threat agents.
S. Alamian, T. Zahraei Salehi, K. Aghaiypour Kolyani, M. Esmaelizad, A. Etemadi   +4 more
doaj   +1 more source

Septin 9 PB domains coordinate centrosome positioning and microtubule acetylation to control epithelial polarity

FEBS Letters, EarlyView.
Septin 9 polybasic domains couple phosphoinositide‐rich membrane binding to centrosome positioning, Golgi organization, and microtubule acetylation to control epithelial polarity. Their loss disrupts this axis, causing centrosome mispositioning, Golgi fragmentation, reduced microtubule acetylation, and polarity inversion via upregulation of the ...
Ting ting Cai, Mohyeddine Omrane, Nassima Benzoubir, Didier Samuel, Ama Gassama‐Diagne   +4 more
wiley   +1 more source

Effect of adjunctive amoxicillin/metronidazole treatment on subgingival Tannerella forsythia, Prevotella intermedia and Fusobacterium nucleatum recolonization in periodontitis patients

Selcuk Dental Journal, 2019
Background: The use of antibiotics adjunctive to non-surgical periodontal treatment is an accepted approach in the treatment of periodontitis. However, bacterial recolonization is a major drawback which complicates the maintenance of therapeutic ...
Emre Yaprak, Uğur Arslan, Tamer Ataoğlu   +2 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source