Results 21 to 30 of about 20,354,300 (293)
Comparison of different real-time PCR chemistries and their suitability for detection and quantification of genetically modified organisms [PDF]
, 2008 Background The real-time polymerase chain reaction is currently the method of choice for quantifying nucleic acids in different DNA based quantification applications. It is widely used also for detecting and quantifying genetically modified components in
Cankar, K. +11 more
core +1 more source
Evaluating an MRNA based body fluid identification test using sybr green fluorescent dye and real-time PCR [PDF]
, 2011 The requirement to have more definitive and wider ranging body fluid identification (BFI) tests has resulted in a range of mRNA based real-time PCR BFI assays utilising Taqman fluorescent dye.
Connolly, Jo-Ann, Williams, Graham
core +2 more sources
The Beginnings of Real-Time PCR [PDF]
Clinical Chemistry, 2009 Featured Article: Heid CA, Stevens J, Livak KJ, Williams PM. Real time quantitative PCR. Genome Res 1996;6:986–94.1 During the mid-1990s the PCR was becoming a mature technology, impacting such areas such as cloning and automated sequencing. Many scientists were making efforts to tame the quantitative power of PCR.
openaire +2 more sources
Simulation of between repeat variability in real time PCR reactions. [PDF]
PLoS ONE, 2012 While many decisions rely on real time quantitative PCR (qPCR) analysis few attempts have hitherto been made to quantify bounds of precision accounting for the various sources of variation involved in the measurement process.
Antoon Lievens +3 more
doaj +1 more source
Real-time PCR Primer sequences.
, 2022 Real-time PCR Primer sequences.
Richard Connor (143488) +7 more
core +1 more source
The Professional Medical Journal, 2012 Abstract Realtime PCR (or qPCR) is a widely used gold of standard for quantitative analysis of gene expression. It is also a very sensitive method for viral copy number detection, and genotyping. This Protocol outlines qPCR theory, data analysis and detailed procedures.
ABIDA RAZA, NAUREEN A KHATTAK
openaire +3 more sources
Development of real-time PCR for detection of Mycoplasma hominis [PDF]
, 2004 Background: Mycoplasma hominis is associated with pelvic inflammatory disease, bacterial vaginosis, post partum fever, sepsis and infections of the central nervous system often leading to serious conditions.
Christiansen, G +19 more
core +1 more source
Lab on a Chip, 2016 World's smallest, fully autonomous, handheld real-time PCR was shown in this contribution. The device can quickly process up to four samples at a time with detection capability of a single DNA copy. The fully integrated system includes all required electronics for fluorescence measurement, data viewing (LCD display) and processing, and is ideal for use
Ahrberg, Christian D. +3 more
openaire +4 more sources
PCR REAL TIME PER L’IDENTIFICAZIONE DI MYCOBACTERIUM TUBERCULOSIS MDR
Microbiologia Medica, 2003 -
F. Meacci +5 more
doaj +1 more source
Detection of Tumor Cell-Specific mRNA in the Peripheral Blood of Patients with Breast Cancer-Evaluation of Several Markers with Real-Time Reverse Transcription-PCR [PDF]
, 2013 It is widely known that cells from epithelial tumors, e. g., breast cancer, detach from their primary tissue and enter blood circulation. We show that the presence of circulating tumor cells (CTCs) in samples of patients with primary and metastatic ...
Simone Hofmann +20 more
core +1 more source