Results 251 to 260 of about 20,354,300 (293)

Identification and validation of reference genes for quantitative real-time PCR in Corydalis saxicola under various abiotic stresses. [PDF]

Sci Rep
Kang L, Wen Z, Liu H, Lu Y, Qin M, Huang L, Lei M, Li C, Zhu D, Li L, Zhang Z.   +10 more
europepmc   +1 more source

Sex determination and genetic screening of equine embryos with whole genome amplification and real-time PCR. [PDF]

J Reprod Dev
McFarlane GR, Backhouse E, Ruiz A, O'Rourke BA, Cortez JV, Grupen CG.   +5 more
europepmc   +1 more source

Development of a qualitative detection method for the pSIM1278 construct in genetically modified potato using real-time PCR. [PDF]

GM Crops Food
Sugino M, Soga K, Narushima J, Matsumoto M, Takabatake R, Yoshiba S, Shibata N.   +6 more
europepmc   +1 more source

A Multiplex One-Tube Nested Real-Time PCR Assay for the Point-of-Care Testing of Infectious Meningitis. [PDF]

Pathogens
Zhang D, Wang J, Zhao Z, Tie Y, Wu J, Jiao S, Liu X, Wang Y, Gao S, Zhao M, Zhao P, Han Z, Lyu X, Shen X, Ma X, Feng Z.   +15 more
europepmc   +1 more source

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Real-Time PCR

Enfermedades infecciosas y microbiologia clinica, 2004
The development of instruments that allow real-time monitoring of fluorescence within PCR reaction vessels is a significant advance in clinical bacteriology. The technology is very flexible, and many alternative instruments and fluorescent probe systems are currently available.
Slade, Neda, Kapitanović, Sanja
  +8 more sources

Real-time PCR

Forensic Science, Medicine, and Pathology, 2007
Research into the field of DNA has increased dramatically in the years since DNA profiling was first identified. Since then, techniques have been developed to identify individuals from DNA samples equivalent to a single cell. Development of these techniques meant that the ability to accurately quantitate the DNA in such samples was required to evolve ...
  +6 more sources

The evolution of real-time PCR machines to real-time PCR chips

Biosensors and Bioelectronics, 2010
Development of Micro-Electro-Mechanical Systems (MEMS) technology has recently allowed the migration of real-time polymerase chain reaction (PCR) machines to lab-on-a-chip systems. The miniaturization of biological instruments has been studied extensively, with several prototypes constructed and tested.
Dasheng, Lee, Pei-Jer, Chen, Gwo-Bin, Lee   +2 more
openaire   +2 more sources

Real-time PCR detection chemistry

Clinica Chimica Acta, 2015
Real-time PCR is the method of choice in many laboratories for diagnostic and food applications. This technology merges the polymerase chain reaction chemistry with the use of fluorescent reporter molecules in order to monitor the production of amplification products during each cycle of the PCR reaction.
E Navarro
exaly   +3 more sources

Real-Time PCR

2015
Wie bereits unter Kap. 13 erwahnt, ist die Quantifizierung der amplifizierten PCR-Produkte nach 25–30 Zyklen nicht mehr sehr einfach. Damit eine Quantifizierung der zu untersuchenden Amplicons (ca. 50–150 bp Lange) ermoglicht werden kann, haben sich verschiedene optische PCR-Systeme zur „online“ Beobachtung des Amplifikationsstatus etabliert (Tab. 14.1)
Hans-Joachim Müller, Daniel Ruben Prange   +1 more
openaire   +2 more sources

Real-Time PCR

2009
Over the past few years there has been a considerable development of DNA amplification by polymerase chain reaction (PCR), and real-time PCR has now superseded conventional PCR techniques in many areas, e.g., the quantification of nucleic acids and genotyping.
A. Evrard, N. Boulle, G.s Lutfalla
openaire   +1 more source