Results 61 to 70 of about 3,905,031 (329)
Multicolor combinatorial probe coding for real-time PCR. [PDF]
PLoS ONE, 2011 The target volume of multiplex real-time PCR assays is limited by the number of fluorescent dyes available and the number of fluorescence acquisition channels present in the PCR instrument.
Qiuying Huang +8 more
doaj +1 more source
Strangles: The Molecular Identification and Epidemiology of Streptococcus equi subsp. equi [PDF]
, 2012 A conventional PCR diagnostic test was established to confirm the microbiological isolation of Streptococcus equi subsp. equi (S. equi), the causative agent of strangles.
Patty, Olivia Anne
core +1 more source
A cost effective real-time PCR for the detection of adenovirus from viral swabs [PDF]
, 2013 Compared to traditional testing strategies, nucleic acid amplification tests such as real-time PCR offer many advantages for the detection of human adenoviruses. However, commercial assays are expensive and cost prohibitive for many clinical laboratories.
Jason J LeBlanc +7 more
core +1 more source
FEBS Letters, EarlyView.This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva +5 more
wiley +1 more source
A simple and cost-effective real-time PCR method using diluted and heat-treated whole blood lysate
Scientific ReportsAlthough DNA extraction is a crucial step before polymerase chain reaction (PCR), attempts to perform PCR without DNA isolation have been ongoing since the 1990s.
Gökçe Güllü Amuran +3 more
doaj +1 more source
Development and verification of PCR based assay to dectect and quantify garden pea lec gene
Czech Journal of Food Sciences, 2012 Genetically modified organisms (GMOs) entering the food chain have became its part, which is necessary to monitor. GMO analyses are used as a control mechanism according to valid acquis communautaire for traceability and labeling of GMOs.
Aleš VRÁBLÍK +4 more
doaj +1 more source
Journal of Agriculture and Food Research, 2023 Panax notoginseng is a traditional Chinese medicinal herb. With its wide applications in medicine, food products, health care, cosmetics and other industries, identification and detection of P. notoginseng have become critical.
Chao Ji +9 more
doaj +1 more source
A real-time PCR-based assay for detection of Wuchereria bancrofti DNA in blood and mosquitoes [PDF]
, 2006 We developed and evaluated real-time polymerase chain reaction (PCR) assays for detecting Wuchereria bancrofti DNA in human blood and in mosquitoes. An assay based on detection of the W.
Atkinson, Laura J +9 more
core +2 more sources
Inhibition mechanisms of hemoglobin, immunoglobulin G, and whole blood in digital and real-time PCR
Analytical and Bioanalytical Chemistry, 2018 Blood samples are widely used for PCR-based DNA analysis in fields such as diagnosis of infectious diseases, cancer diagnostics, and forensic genetics. In this study, the mechanisms behind blood-induced PCR inhibition were evaluated by use of whole blood
Maja Sidstedt +7 more
semanticscholar +1 more source
Sequence determinants of RNA G‐quadruplex unfolding by Arg‐rich regions
FEBS Letters, EarlyView.We show that Arg‐rich peptides selectively unfold RNA G‐quadruplexes, but not RNA stem‐loops or DNA/RNA duplexes. This length‐dependent activity is inhibited by acidic residues and is conserved among SR and SR‐related proteins (SRSF1, SRSF3, SRSF9, U1‐70K, and U2AF1).
Naiduwadura Ivon Upekala De Silva +10 more
wiley +1 more source