Results 71 to 80 of about 3,905,031 (329)

Evaluation of VERSANT® CT/GC DNA 1.0 Assay in conjunction with VERSANT® kPCR Molecular system

Microbiologia Medica, 2011
A total of 463 first void urine specimens were obtained from patients attending the Sexually Transmitted Diseases (STD) Outpatients Clinic of the St. Orsola University Hospital in Bologna, Italy. Samples were tested by the new test VERSANT® CT/GC DNA 1.0
Antonella Marangoni, Alessandra Moroni, Antonietta D’Antuono, Nicoletta Banzola, Enzo Della Bella, Claudio Foschi, Paola Nardini, Paola Monari, Manuela Donati, Roberto Cevenini   +9 more
doaj   +1 more source

Effects of Flight on Gene Expression and Aging in the Honey Bee Brain and Flight Muscle [PDF]

, 2012
Honey bees move through a series of in-hive tasks (e.g., “nursing”) to outside tasks (e.g., “foraging”) that are coincident with physiological changes and higher levels of metabolic activity.
Ammons, Andrew, Benito, Azucena A., Elekonich, Michelle M., Mancinelli, Georgina E., Margotta, Joseph, Roberts, Stephen P.   +5 more
core   +5 more sources

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

Detection of Leishmania infantum in Lutzomyia longipalpis captured in Campo Grande, MS

Revista Brasileira de Parasitologia Veterinária
Leishmaniasis is a zoonotic disease caused by protozoa of the genus Leishmania (Ross, 1903) and is the focus of considerable attention in human and veterinary medicine.
Rodrigo Casquero Cunha, Renato Andreotti, Marlon Cezar Cominetti, Elaine Araújo Silva   +3 more
doaj   +1 more source

Real-time PCR: A rapid and sensitive method for diagnosis of dermatophyte induced onychomycosis, a comparative study

Alexandria Journal of Medicine, 2016
Onychomycosis is the most common nail disorder, accounting for up to 50% of all nail problems and about 30% of cutaneous fungal infections. Treatment of onychomycosis is expensive.
Sherin M. Emam, Osama H. Abd El-salam
doaj   +1 more source

A prospective observational study of mycophenolate mofetil treatment in progressive diffuse cutaneous systemic sclerosis of recent onset. [PDF]

, 2012
OBJECTIVE: A prospective observational study of mycophenolate mofetil (MMF) treatment in patients with diffuse progressive cutaneous systemic sclerosis (SSc) of recent onset.
Jimenez, Sergio A., Lee, MD, Jason B., Mendoza, MD, Fabian A., Nagle, Sarah J.   +3 more
core   +3 more sources

Reference genes in real-time PCR

Journal of Applied Genetics, 2013
This paper aims to discuss various aspects of the use of reference genes in qPCR technique used in the thousands of present studies. Most frequently, these are housekeeping genes and they must meet several criteria so that they can lay claim to the name.
Bartłomiej Kozera, M. Rapacz
semanticscholar   +1 more source

β‐TrCP overexpression enhances cisplatin sensitivity by depleting BRCA1

Molecular Oncology, EarlyView.
Low levels of β‐TrCP (Panel A) allow the accumulation of BRCA1 and CtIP, which facilitate the repair of cisplatin‐induced DNA damage via homologous recombination (HR) and promote tumor cell survival. In contrast, high β‐TrCP expression (Panel B) leads to BRCA1 and CtIP degradation, impairing HR repair, resulting in persistent DNA damage and apoptosis ...
Rocío Jiménez‐Guerrero, Alejandro Belmonte‐Fernández, Mónica González‐Moreno, Laura Rodríguez‐Cordero, Begoña Pérez‐Valderrama, Joaquín Herrero‐Ruíz, Francisco Romero, Carmen Sáez, Miguel Á. Japón   +8 more
wiley   +1 more source

Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions [PDF]

, 2003
BACKGROUND: Real-time PCR is increasingly being adopted for RNA quantification and genetic analysis. At present the most popular real-time PCR assay is based on the hybridisation of a dual-labelled probe to the PCR product, and the development of a ...
Bell, S.M., Bransfield, K., Combaret, V., Douglas, S.H., Field, S.L., Inglehearn, C.F., Isaacs, J.D., Leong, F.T., Markham, A.F., Mighell, A.J., Ponchel, F., Puisieux, A., Robinson, P.A., Toomes, C.   +13 more
core   +3 more sources

Nicotinamide N‐methyltransferase promotes drug resistance in lung cancer, as revealed by nascent proteomic profiling

Molecular Oncology, EarlyView.
AZD9291 has shown promise in targeted cancer therapy but is limited by resistance. In this study, we employed metabolic labeling and LC–MS/MS to profile time‐resolved nascent protein perturbations, allowing dynamic tracking of drug‐responsive proteins. We demonstrated that increased NNMT expression is associated with drug resistance, highlighting NNMT ...
Zhanwu Hou, Zhen Wang, Fei Yang, Xiao Han, Lei Li, Huadong Liu   +5 more
wiley   +1 more source