Results 121 to 130 of about 18,071 (160)
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SOP EMrtCO1 realtime PCR for the detection of Echinococcus multilocularis eggs

2021
OHEJP MEmE This method describes the molecular identification of Echinococcus multilocularis DNA using a realtime PCR method with a CO1 target. Probe used is FAM as a reporter dye in assay, IBFQ as quencher, and a ZEN internal quenching modification to the probe in order to lower background and increase signal detection.
RK, Davidson, Øines Ø, Henriksen K
openaire   +2 more sources

New realtime PCR method development for detection and quantification of Bacillus subtilis

Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam, 2022
Bacillus subtilis is a widely used bacterial strain in probiotic production. The strains belong to this species are endosporogenic, tolerant to acidic pH conditions in the stomach, and many have been shown to produce enzymes that aid in food digestion and inhibit pathogenic microorganisms.
Xuan Huong Nguyen Thi   +7 more
openaire   +1 more source

mRNA profiling for body fluid identification by reverse transcription endpoint PCR and realtime PCR

Forensic Science International: Genetics, 2009
mRNA profiling is a promising new method for the identification of body fluids from biological stains. Major advantages of mRNA profiling are the possibility of detecting several body fluids in one multiplex reaction and of simultaneously isolating DNA without loss of material. A reverse transcription endpoint polymerase chain reaction (PCR) method and
Haas, C   +4 more
openaire   +4 more sources

APPLICATION OF REALTIME PCR FOR DETECTING M.TUBERCULOSIS RESISTANT TO RIFAMPICIN AND ISONIAZID

Journal of Medicine and Pharmacy, 2015
Introduction: Conventional culture methods for antibiotic susceptibility testing for M. tuberculosis may take days to several weeks. Use of realtime PCR for rapid detection of drug and multidrug resistant tuberculosis could facilitate early initiation of appropriate anti-tubercular treatment regimen thereby interrupting transmission.
Viet Quynh Tram Ngo   +3 more
openaire   +1 more source

THE USE OF REALTIME PCR IN THE MONITORING OF PATHOLOGY OF PIGS CAUSED BY THE CIRCOVIRUS OF THE SECOND TYPE

Epizootology Immunobiology Pharmacology Sanitation, 2022
The article presents data on the persistence of type 2 circovirus in one of the farms of the Republic of Belarus. The presence of the pathogen genome in pigs of different ages was established, and comparative data on the results of PCR and ELISA were presented.
E. L. Krasnikova, A. L. Sadovsky
openaire   +1 more source

DETECTION OF RSV IN PEDIATRIC PATIENTS UNDER 5 YEARS OLD BY REALTIME RT-PCR AND CONVENTIONAL RT-PCR

Journal of Medicine and Pharmacy, 2015
Objective: Application of realtime RT-PCR and conventional RT-PCR for detection of RSV in viral respiratory infections. Methods: Realtime RT-PCR and conventional nested RT-PCR were used to detect RSV on 110 respiratory samples collected from children with viral lower respiratory infections.
Van An Le   +3 more
openaire   +1 more source

Analytical performances of real-time PCR by Abbott RealTime CMV with m2000 for the detection of cytomegalovirus in urine

Annales de biologie clinique, 2016
Congenital cytomegalovirus (CMV) infection is the leading cause of sensoneurinal disability due to infectious congenital disease. The diagnosis of congenital CMV infection is based on the search of CMV in the urine within the first two weeks of life. Viral culture of urine is the gold standard. However, the PCR is highly sensitive and faster.
Anne, De Monte   +4 more
openaire   +2 more sources

Detection of tomato mosaic virus infecting tomato using realtime RT-PCR

The Journal of Agriculture and Development
Tomato mosaic virus (ToMV) is known as one of the most common and devastating tomato viruses worldwide. It causes mosaic disease, which significantly impacts the productivity and quality of tomatoes in Vietnam. Early and accurate detection of ToMV in tomatoes is essential for effective disease control.
Quyen N. D. Cao   +2 more
openaire   +1 more source

Entwicklung von Realtime-PCR-Methoden zur Analyse von DNA-Methylierung

2007
DNA-Methylation plays a role in a number of biological processes, such as imprinting, X chromosomal inactivation and gene expression. DNA hypermethylation is closely associated with transcriptional silencing, while DNA hypomethylation is associated with transcriptional activation. The tumor genesis is associated with changes in DNA methylation patterns
openaire   +1 more source

Realtime PCR on urine sediments in the diagnosis of trichomoniasis in male subjects

International Journal of STD & AIDS, 2006
Versluis, Y   +3 more
openaire   +2 more sources

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