Results 161 to 170 of about 32,034 (209)
Dynamics of antiretroviral restriction factor expression in sheep naturally infected with Small ruminant lentivirus (SRLV). [PDF]
Arch VirolRodríguez-Murillo C +4 more
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mRNA profiling for body fluid identification by reverse transcription endpoint PCR and realtime PCR
Forensic Science International: Genetics, 2009mRNA profiling is a promising new method for the identification of body fluids from biological stains. Major advantages of mRNA profiling are the possibility of detecting several body fluids in one multiplex reaction and of simultaneously isolating DNA without loss of material. A reverse transcription endpoint polymerase chain reaction (PCR) method and
Haas, C +4 more
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New realtime PCR method development for detection and quantification of Bacillus subtilis
Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam, 2022Bacillus subtilis is a widely used bacterial strain in probiotic production. The strains belong to this species are endosporogenic, tolerant to acidic pH conditions in the stomach, and many have been shown to produce enzymes that aid in food digestion and inhibit pathogenic microorganisms.
Xuan Huong Nguyen Thi +7 more
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SOP EMrtCO1 realtime PCR for the detection of Echinococcus multilocularis eggs
2021OHEJP MEmE This method describes the molecular identification of Echinococcus multilocularis DNA using a realtime PCR method with a CO1 target. Probe used is FAM as a reporter dye in assay, IBFQ as quencher, and a ZEN internal quenching modification to the probe in order to lower background and increase signal detection.
RK, Davidson, Øines Ø, Henriksen K
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Xây dựng quy trình realtime RT-PCR phát hiện Zika và Chikungunya
Journal of 108 - Clinical Medicine and Phamarcy, 2021Mục tiêu: Xây dựng quy trình realtime RT-PCR phát hiện Zika (ZIKV) và Chikungunya (CHIKV). Đối tượng và phương pháp: Mẫu chứng dương ZIKV do NIHE, CHIKV do Tổ chức Y tế Thế giới (WHO) cung cấp và các virus khác được sử dụng trong nghiên cứu này. Để đánh giá ngưỡng phát hiện, độ nhạy kỹ thuật realtime RT-PCR, nghiên cứu sử dụng mẫu RNA chứng dương pha ...
Đào Thanh Quyên +11 more
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Convection-based realtime polymerase chain reaction (PCR) utilizing transparent graphene heaters
IEEE SENSORS 2014 Proceedings, 2014We present a polymerase chain reaction (PCR) chip incorporating graphene heaters and thermal convection. We adopted graphene heaters in order to replace the bulky metal heating blocks or opaque metal line heaters utilized in the previous PCR chips. Transparency of the CVD based graphene heaters allowed the realtime fluorescence detection of PCR product
Kwang Hyo Chung +7 more
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APPLICATION OF REALTIME PCR FOR DETECTING M.TUBERCULOSIS RESISTANT TO RIFAMPICIN AND ISONIAZID
Journal of Medicine and Pharmacy, 2015Introduction: Conventional culture methods for antibiotic susceptibility testing for M. tuberculosis may take days to several weeks. Use of realtime PCR for rapid detection of drug and multidrug resistant tuberculosis could facilitate early initiation of appropriate anti-tubercular treatment regimen thereby interrupting transmission.
Viet Quynh Tram Ngo +3 more
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Entwicklung von Realtime-PCR-Methoden zur Analyse von DNA-Methylierung
2007DNA-Methylation plays a role in a number of biological processes, such as imprinting, X chromosomal inactivation and gene expression. DNA hypermethylation is closely associated with transcriptional silencing, while DNA hypomethylation is associated with transcriptional activation. The tumor genesis is associated with changes in DNA methylation patterns
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2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems & Eurosensors XXXIII (TRANSDUCERS & EUROSENSORS XXXIII), 2019
This paper reports a handheld and battery-powered polymerase chain reaction (PCR) system using a polyimide film-based microfabricated heating modules and polymer film microfluidic chambers, with a very low power consumption, compared to state of the art. The device will be, to the best of our knowledge, the smallest and very fast real-time microfluidic
Dae-Sik Lee, Ok Ran Choi, YuJin Seo
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This paper reports a handheld and battery-powered polymerase chain reaction (PCR) system using a polyimide film-based microfabricated heating modules and polymer film microfluidic chambers, with a very low power consumption, compared to state of the art. The device will be, to the best of our knowledge, the smallest and very fast real-time microfluidic
Dae-Sik Lee, Ok Ran Choi, YuJin Seo
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DETECTION OF RSV IN PEDIATRIC PATIENTS UNDER 5 YEARS OLD BY REALTIME RT-PCR AND CONVENTIONAL RT-PCR
Journal of Medicine and Pharmacy, 2015Objective: Application of realtime RT-PCR and conventional RT-PCR for detection of RSV in viral respiratory infections. Methods: Realtime RT-PCR and conventional nested RT-PCR were used to detect RSV on 110 respiratory samples collected from children with viral lower respiratory infections.
Van An Le +3 more
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