Application of Comparative Genomics for the Development of PCR Primers for the Detection of Harmful or Beneficial Microorganisms in Food: Mini-Review. [PDF]
FoodsKim SS.
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NMDAR inhibitor preconditioned mesenchymal stromal cell-derived extracellular vesicles enhance post-stroke recovery by targeting excitotoxicity and neuronal regeneration. [PDF]
Front Cell NeurosciZhang X, Tian H, Bo H, Zhong L.
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Nitric oxide-dependent stabilization of vimentin confers chemoresistance in ovarian cancer. [PDF]
Mol Ther Nucleic AcidsMelone G +21 more
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New realtime PCR method development for detection and quantification of Bacillus subtilis
Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam, 2022Bacillus subtilis is a widely used bacterial strain in probiotic production. The strains belong to this species are endosporogenic, tolerant to acidic pH conditions in the stomach, and many have been shown to produce enzymes that aid in food digestion and inhibit pathogenic microorganisms.
Xuan Huong Nguyen Thi +7 more
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SOP EMrtCO1 realtime PCR for the detection of Echinococcus multilocularis eggs
2021OHEJP MEmE This method describes the molecular identification of Echinococcus multilocularis DNA using a realtime PCR method with a CO1 target. Probe used is FAM as a reporter dye in assay, IBFQ as quencher, and a ZEN internal quenching modification to the probe in order to lower background and increase signal detection.
RK, Davidson, Øines Ø, Henriksen K
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mRNA profiling for body fluid identification by reverse transcription endpoint PCR and realtime PCR
Forensic Science International: Genetics, 2009mRNA profiling is a promising new method for the identification of body fluids from biological stains. Major advantages of mRNA profiling are the possibility of detecting several body fluids in one multiplex reaction and of simultaneously isolating DNA without loss of material. A reverse transcription endpoint polymerase chain reaction (PCR) method and
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APPLICATION OF REALTIME PCR FOR DETECTING M.TUBERCULOSIS RESISTANT TO RIFAMPICIN AND ISONIAZID
Journal of Medicine and Pharmacy, 2015Introduction: Conventional culture methods for antibiotic susceptibility testing for M. tuberculosis may take days to several weeks. Use of realtime PCR for rapid detection of drug and multidrug resistant tuberculosis could facilitate early initiation of appropriate anti-tubercular treatment regimen thereby interrupting transmission.
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The article presents data on the persistence of type 2 circovirus in one of the farms of the Republic of Belarus. The presence of the pathogen genome in pigs of different ages was established, and comparative data on the results of PCR and ELISA were presented.
E. L. Krasnikova, A. L. Sadovsky
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DETECTION OF RSV IN PEDIATRIC PATIENTS UNDER 5 YEARS OLD BY REALTIME RT-PCR AND CONVENTIONAL RT-PCR
Journal of Medicine and Pharmacy, 2015Objective: Application of realtime RT-PCR and conventional RT-PCR for detection of RSV in viral respiratory infections. Methods: Realtime RT-PCR and conventional nested RT-PCR were used to detect RSV on 110 respiratory samples collected from children with viral lower respiratory infections.
Van An Le +3 more
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Congenital cytomegalovirus (CMV) infection is the leading cause of sensoneurinal disability due to infectious congenital disease. The diagnosis of congenital CMV infection is based on the search of CMV in the urine within the first two weeks of life. Viral culture of urine is the gold standard. However, the PCR is highly sensitive and faster.
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