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Storage of Recalcitrant Seeds

2020
The Indo-Malaysian rainforest is renowned for its great diversity of tropical flora. It has over 25,000 species of flowering plants and is one of the World’s major centers of origin of many species of fruit trees, timber, spices, and medicinal plants.
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The Basis of Recalcitrant Seed Behaviour

1989
The term ‘recalcitrance’, defined as obstinate disobedience, refers to seeds that undergo no maturation drying as the final phase of development, tolerate very little post-shedding desiccation and are often chilling-sensitive. Such seeds are unstorable by any of the methods used for air-dry orthodox seeds.
Patricia Berjak   +2 more
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The ascorbate system in recalcitrant and orthodox seeds

Physiologia Plantarum, 1999
Recalcitrant seeds of Ginkgo biloba L., Quercus cerris L., Aesculus hippocastanum L. and Cycasrevoluta Thunb. are shed by the plant at a high moisture content, contain a large amount of ascorbic acid (ASA) and maintain high ascorbate (ASC) peroxidase (EC 1.11.1.11) activity. Three proteins showing ASC peroxidase activity are present in G. biloba seeds.
TOMMASI, Franca   +2 more
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Cryopreservation of Recalcitrant Seeds

1995
Long-term ex situ storage of plant germplasm is of increasing importance, both for maintaining the genetic diversity of species with current human use, and for the preservation of species threatened with extinction in the wild. Seed banks have long been used for this purpose, taking advantage of a natural plant preservation mechanism: the dry seed ...
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Evolutionary and ecological aspects of recalcitrant seed biology

Seed Science Research, 2000
AbstractThere is a substantial literature on the basic physiology and response to desiccation of recalcitrant seeds, but little is known about their ecology and even less of their evolutionary status. It is difficult to assess the response of early land plants to dehydration, but it is likely that desiccation tolerance in vegetative tissue arose ...
N.W. Pammenter, Patricia Berjak
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ECOLOGICAL SIGNIFICANCE OF RECALCITRANT SEEDS

Recalcitrant seeds, characterized by their sensitivity to d esiccation and inability to withstandfreezing temperatures, play a critical role in maintaining biodiversity and ecosystemfunctionality. Unlike orthodox seeds, recalcitrant seeds germinate rapidly after dispersal,allowing them to adapt to specific envir onmental conditions, particularly in ...
Chamoli, Vinay   +4 more
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The Ecology and Physiology of Viviparous and Recalcitrant Seeds

Annual Review of Ecology and Systematics, 2000
▪ Abstract  Understanding seed physiology is central to reconstructing how angiosperms have evolved, to characterizing dormancy and germination regimes shared by suites of species, and to devising sound strategies for seed bank conservation, agriculture, and forestry.
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Recalcitrant seeds of horse chestnut lack protein bodies

Russian Journal of Plant Physiology, 2016
In recalcitrant seeds of horse chestnut (Aesculus hippocastanum L.), the bulk of protein in axial organs and cotyledons is accounted for by water-soluble proteins (albumins). In the cells of embryo, proteins are predominantly located in the cytosol, whereas the fraction of cell structures precipitate in the range from 1000 to 20000 g, accounting for ...
M. I. Azarkovich, Yu. P. Bolyakina
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Identification and characterization of dehydrins in horse chestnut recalcitrant seeds

Russian Journal of Plant Physiology, 2010
The fraction of heat-stable dehydrins cytosolic proteins from mature recalcitrant seeds of horse chestnut (Aesculus hippocastanum L.) was studied in the period of their dormancy and germination in order to identify and characterize stress-induced dehydrin-like polypeptides.
N. A. Gumilevskaya, M. I. Azarkovich
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Cryopreservation of tropical recalcitrant-seeded species – perspectives

Acta Horticulturae, 2019
Cryopreservation is an alternative strategy for the long-term conservation of tropical recalcitrant-seeded species. Embryonic axes/embryos or shoot tips are possible explants for this purpose as the seeds are sensitive to cold temperature and they are not tolerant to desiccation to a low moisture content required for seed banking.
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