Results 1 to 10 of about 118,083 (181)

Cellular-level versus receptor-level response threshold hierarchies in T-cell activation [PDF]

Frontiers in Immunology, 2013
Peptide-MHC (pMHC) ligand engagement by T-cell receptors (TCRs) elicits a variety of cellular responses, some of which require substantially more TCR-mediated stimulation than others.
Hugo Antonius van den Berg, Kristin eLadell, Kelly eMiners, Bruno eLaugel, Sian eLlewellyn-Lacey, Mathew eClement, David K Cole, Emma eGostick, Linda eWooldridge, Andrew Kelvin Sewell, John Stephen Bridgeman, David A Price, David A Price   +12 more
doaj   +9 more sources

Opto-APC: Engineering of cells that display phytochrome B on their surface for optogenetic studies of cell-cell interactions

Frontiers in Molecular Biosciences, 2023
The kinetics of a ligand-receptor interaction determine the responses of the receptor-expressing cell. One approach to experimentally and reversibly change this kinetics on demand is optogenetics.
Marissa Russ, Marissa Russ, Anna K. Ehret, Anna K. Ehret, Anna K. Ehret, Maximilian Hörner, Daniel Peschkov, Rebecca Bohnert, Vincent Idstein, Vincent Idstein, Vincent Idstein, Susana Minguet, Susana Minguet, Wilfried Weber, Björn F. Lillemeier, O. Sascha Yousefi, Wolfgang W. Schamel, Wolfgang W. Schamel   +17 more
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Live-cell microscopy or fluorescence anisotropy with budded baculoviruses—which way to go with measuring ligand binding to M4 muscarinic receptors?

Open Biology, 2022
M4 muscarinic acetylcholine receptor is a G protein-coupled receptor (GPCR) that has been associated with alcohol and cocaine abuse, Alzheimer's disease, and schizophrenia which makes it an interesting drug target.
Maris-Johanna Tahk, Jane Torp, Mohammed A. S. Ali, Dmytro Fishman, Leopold Parts, Lukas Grätz, Christoph Müller, Max Keller, Santa Veiksina, Tõnis Laasfeld, Ago Rinken   +10 more
doaj   +1 more source

A tunable coarse-grained model for ligand-receptor interaction. [PDF]

PLoS Computational Biology, 2013
Cell-surface receptors are the most common target for therapeutic drugs. The design and optimization of next generation synthetic drugs require a detailed understanding of the interaction with their corresponding receptors. Mathematical approximations to
Teresa Ruiz-Herrero, Javier Estrada, Javier Estrada, Raúl Guantes, David G Miguez   +4 more
doaj   +1 more source

Fluorescence based HTS-compatible ligand binding assays for dopamine D3 receptors in baculovirus preparations and live cells

Frontiers in Molecular Biosciences, 2023
Dopamine receptors are G-protein-coupled receptors that are connected to severe neurological disorders. The development of new ligands targeting these receptors enables gaining a deeper insight into the receptor functioning, including binding mechanisms,
Maris-Johanna Tahk, Tõnis Laasfeld, Tõnis Laasfeld, Elo Meriste, Jose Brea, Maria Isabel Loza, Maria Majellaro, Maria Majellaro, Marialessandra Contino, Eddy Sotelo, Ago Rinken   +10 more
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Two-dimensional measurements of receptor-ligand interactions

Frontiers in Molecular Biosciences, 2023
Gaining insight into the two-dimensional receptor-ligand interactions, which play a significant role in various pivotal biological processes such as immune response and cancer metastasis, will deepen our understanding of numerous physiological and ...
Songjie Zheng, Songjie Zheng, Min Zou, Min Zou, Yingfeng Shao, Huaping Wu, Helong Wu, Xiaohuan Wang   +7 more
doaj   +1 more source

Single-molecule Force Spectroscopy on Biomembrane Force Probe to Characterize Force-dependent Bond Lifetimes of Receptor–ligand Interactions on Living Cells

Bio-Protocol, 2022
The transmembrane receptor–ligand interactions play a vital role in the physiological and pathological processes of living cells, such as immune cell activation, neural synapse formation, or viral invasion into host cells. Mounting evidence suggests that
Tongtong Zhang, Chenyi An, Wei Hu, Wei Chen   +3 more
doaj   +1 more source

NOTCH3-targeted antibody drug conjugates regress tumors by inducing apoptosis in receptor cells and through transendocytosis into ligand cells

Cell Reports Medicine, 2021
Summary: Aberrant NOTCH3 signaling and overexpression is oncogenic, associated with cancer stem cells and drug resistance, yet therapeutic targeting remains elusive.
Kenneth G. Geles, Yijie Gao, Andreas Giannakou, Latha Sridharan, Ting-Ting Yamin, Jing Zhang, Riyez Karim, Joel Bard, Nicole Piche-Nicholas, Manoj Charati, Andreas Maderna, Judy Lucas, Jonathon Golas, Magali Guffroy, Steven Pirie-Shepherd, Marc Roy, Jessie Qian, Tania Franks, Wenyan Zhong, Christopher J. O’Donnell, Lioudmila Tchistiakova, Hans-Peter Gerber, Puja Sapra   +22 more
doaj   +1 more source

Bell-Evans model and steered molecular dynamics in uncovering the dissociation kinetics of ligands targeting G-protein-coupled receptors

Scientific Reports, 2022
Recently, academic and industrial scientific communities involved in kinetics-based drug development have become immensely interested in predicting the drug target residence time.
Muhammad Jan Akhunzada, Hyun Jung Yoon, Indrajit Deb, Abdennour Braka, Sangwook Wu   +4 more
doaj   +1 more source

Insights into intercellular receptor-ligand binding kinetics in cell communication

Frontiers in Bioengineering and Biotechnology, 2022
Cell-cell communication is crucial for cells to sense, respond and adapt to environmental cues and stimuli. The intercellular communication process, which involves multiple length scales, is mediated by the specific binding of membrane-anchored receptors
Chenyi An, Chenyi An, Xiaohuan Wang, Fan Song, Fan Song, Jinglei Hu, Long Li   +6 more
doaj   +1 more source