Results 31 to 40 of about 118,083 (181)

Sensitivity analysis of intracellular signaling pathway kinetics predicts targets for stem cell fate control. [PDF]

PLoS Computational Biology, 2007
Directing stem cell fate requires knowledge of how signaling networks integrate temporally and spatially segregated stimuli. We developed and validated a computational model of signal transducer and activator of transcription-3 (Stat3) pathway kinetics ...
Alborz Mahdavi, Ryan E Davey, Patrick Bhola, Ting Yin, Peter W Zandstra   +4 more
doaj   +1 more source

Functional selectivity of GPCR-directed drug action through location bias. [PDF]

, 2017
G-protein-coupled receptors (GPCRs) are increasingly recognized to operate from intracellular membranes as well as the plasma membrane. The β 2 -adrenergic GPCR can activate G s -linked cyclic AMP (G s -cAMP) signaling from endosomes.
Conti, Marco, Huang, Bo, Irannejad, Roshanak, Mika, Delphine, Pessino, Veronica, von Zastrow, Mark, Wedegaertner, Philip B.   +6 more
core   +3 more sources

Receptor oligomerization and beyond: a case study in bone morphogenetic proteins

BMC Biology, 2009
Background Transforming growth factor (TGF)β superfamily members transduce signals by oligomerizing two classes of serine/threonine kinase receptors, termed type I and type II. In contrast to the large number of ligands only seven type I and five type II
Mueller Thomas D, Schmitz Werner, Seher Axel, Heinecke Kai, Sebald Walter, Nickel Joachim   +5 more
doaj   +1 more source

The Roles of Membrane Rafts in CD32A-Mediated Phagocytosis [PDF]

, 2007
Membrane rafts are highly dynamic heterogeneous sterol- and sphingolipid-rich micro-domains on cell surfaces. They are generally believed to provide residency for cell surface molecules (e.g., adhesion and signaling molecules) and scaffolding to ...
Cheng Zhu, Periasamy Selvaraj, Timothy P. Tolentino   +2 more
core   +2 more sources

The role of kinetic context in apparent biased agonism at GPCRs

Nature Communications, 2016
Biased agonists act at a receptor to preferentially induce distinct intracellular signalling responses over others. Here the authors show how kinetics of ligand binding and signaling responses greatly influence observed bias profiles, and hence must be ...
Carmen Klein Herenbrink, David A. Sykes, Prashant Donthamsetti, Meritxell Canals, Thomas Coudrat, Jeremy Shonberg, Peter J. Scammells, Ben Capuano, Patrick M. Sexton, Steven J. Charlton, Jonathan A. Javitch, Arthur Christopoulos, J. Robert Lane   +12 more
doaj   +1 more source

Micro-pharmacokinetics: Quantifying local drug concentration at live cell membranes

Scientific Reports, 2018
Fundamental equations for determining pharmacological parameters, such as the binding affinity of a ligand for its target receptor, assume a homogeneous distribution of ligand, with concentrations in the immediate vicinity of the receptor being the same ...
Karolina Gherbi, Stephen J. Briddon, Steven J. Charlton   +2 more
doaj   +1 more source

L-selectin mediated leukocyte tethering in shear flow is controlled by multiple contacts and cytoskeletal anchorage facilitating fast rebinding events

, 2004
L-selectin mediated tethers result in leukocyte rolling only above a threshold in shear. Here we present biophysical modeling based on recently published data from flow chamber experiments (Dwir et al., J. Cell Biol.
Alon, Alon, Bell, Chan, Chang, Chen, de Ch  teau, Doggett, Erdmann, Evans, Finger, Greenberg, Hynes, Kansas, Kucik, Marshall, Nicholson, Pavalko, R. Alon, Shao, Shoup, Springer, U. S. Schwarz   +22 more
core   +2 more sources

Function‐driven design of a surrogate interleukin‐2 receptor ligand

FEBS Letters, EarlyView.
Interleukin (IL)‐2 signaling can be achieved and precisely fine‐tuned through the affinity, distance, and orientation of the heterodimeric receptors with their ligands. We designed a biased IL‐2 surrogate ligand that selectively promotes effector T and natural killer cell activation and differentiation. Interleukin (IL) receptors play a pivotal role in
Ziwei Tang, Zelin Cheng, Teng Li, Fulian Wang, Liangminghui Zhang, Xiuxiu He, Lili Liu, Wei Wang, Aibin Liang, Guang Yang   +9 more
wiley   +1 more source

Progressive enhancement of kinetic proofreading in T cell antigen discrimination from receptor activation to DAG generation

eLife, 2022
T cells use kinetic proofreading to discriminate antigens by converting small changes in antigen-binding lifetime into large differences in cell activation, but where in the signaling cascade this computation is performed is unknown.
Derek M Britain, Jason P Town, Orion David Weiner   +2 more
doaj   +1 more source

Chloroplast and Cell Imaging at Submicron Resolution by Two-Photon Excitation [PDF]

, 2012
Novel, two-photon NIR excitation fluorescence correlation microspectroscopy tests and preliminary results were presented in this article with submicron resolution for concentrated suspensions of functional cells and chloroplast membranes.
I C. Baianu
core   +1 more source